Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3185-3193.DOI: 10.11733/j.issn.1007-0435.2025.10.006

Previous Articles    

Analysis of Genetic Diversity and Population Structure of Oat Germplasm Resources Based on SLAF-Seq

KANG Jia-hui, ZHENG Min-na, YANG Fu, WANG Li, ZHANG Zhi   

  1. High Latitude Crops Institute, Shanxi Agricultural University, Datong, Shanxi Province 037008, China
  • Received:2025-07-02 Revised:2025-08-15 Published:2025-10-17

基于SLAF-Seq的燕麦种质资源遗传多样性分析和群体结构分析

康佳惠, 郑敏娜, 杨富, 王力, 张知   

  1. 山西农业大学高寒区作物研究所, 山西 大同 037008
  • 通讯作者: 郑敏娜,E-mail:zhengminna@126.com
  • 作者简介:郑敏娜,E-mail:zhengminna@126.com
  • 基金资助:
    国家重点研发计划项目子课题（2023YFD1202703-6）；山西省基础研究计划项目（202303021222076）；大同市应用基础研究计划项目（2024062）；山西农业大学生物育种工程项目（YZGC085）；山西农业大学科技创新提升工程（CXGC2023076）；杂粮、杂豆水肥一体化技术集成示范（TSZX25-17）资助

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Abstract: To understand the genetic diversity among different groups and the relationship of population variation， and to provide a genetic background basis for the innovation of oat （Avena sativa L.） germplasm， this study used 98 oat germplasm resources from different geographical origins as materials and utilized the specific length amplified fragment sequencing （SLAF-Seq） technology to screen single nucleotide polymorphism （SNP） molecular markers in the whole genome. The results showed that based on 1470.85 Mb of high-quality sequencing data， a total of 626 992 SLAF tags （average sequencing depth of 10.81x） were detected， and 516 005 polymorphic loci were developed， with 8 109 634 high-quality population SNPs screened out， and the overall heterozygosity rate was 2.82%. SNP markers were non-randomly distributed on 27 chromosomes， with significant enrichment regions on chromosomes 4 and 5. Population structure analysis showed that the genetic diversity level of the oat population in this study was relatively high， and the degree of genetic differentiation among populations was large. Except for S9 （‘Zhangyou 15’） having a medium to high level of kinship with S8 （‘Baiyan 2’）， S17 （‘Baiyan 20’）， and S23 （‘Guyan 1’）， and S70 （‘Qinghai 444’） having a medium to high level of kinship with S83 （201430-8-1）， S69 （‘Sicao 10’）， and S82 （201406-12）， the genetic relationship among the other germplasm resources was relatively distant.

Key words: Oats, SLAF-seq, SNP, Genetic diversity analysis, Group structure analysis

摘要： 为了解不同群体间的遗传多样性及群体变异关系，为燕麦（Avena sativa L.）种质创新提供遗传背景依据，本研究以不同地理来源的98份燕麦种质资源为材料，利用特异性位点扩增片段测序技术（Specific Length Amplified Fragment Sequencing，SLAF-Seq）在全基因组范围筛选单核苷酸多态性（Single Nucleotide Polymorphism，SNP）分子标记。结果表明：基于1470.85 Mb高质量测序数据，共检测到626 992个SLAF标签（平均测序深度10.81x），开发出516 005个多态性位点，筛选获得8 109 634个高质量群体SNP，整体杂合率达2.82%；SNP标记在27条染色体上非随机分布，其中，第4号和第5号染色体存在显著富集区；群体结构分析显示本研究中燕麦群体遗传多样性水平较高，群体间遗传分化程度较大，除S9（‘张莜15号’）与S8（‘白燕2号’）、S17（‘白燕20号’）、S23（‘固燕1号’）具有中高水平的近亲关系，S70（‘青海444’）与S83（201430-8-1）、S69（‘饲草10号’）、S82（201406-12）具有中高水平的近亲关系，其余各种质资源间的亲缘关系较远。

关键词: 燕麦, SLAF-seq, SNP, 遗传多样性分析, 群体结构分析

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