Effects of Rhizobial Inoculation on Growth and Physiology of Forage Soybean at Different Nitrogen Levels

doi:10.11733/j.issn.1007-0435.2025.11.035

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3847-3858.DOI: 10.11733/j.issn.1007-0435.2025.11.035

Effects of Rhizobial Inoculation on Growth and Physiology of Forage Soybean at Different Nitrogen Levels

TIAN Yu, WANG Ming-jiu, WANG Zheng-qing, LI Shu-ning, ZHAO Wei-xuan, JIA Yun-peng

1. College of Grassland Science/Inner Mongolia Agricultural University/Key Laboratory of Grassland Resources, Ministryof Education, Hohhot, Inner Mongolia 010018, China

Received:2024-12-17 Revised:2025-02-23 Published:2025-11-13

不同氮素水平下接种根瘤菌对饲用大豆生长和生理的影响

田雨, 王明玖, 王正卿, 李舒宁, 赵伟璇, 贾云鹏

1. 内蒙古农业大学草业学院/草地资源教育部重点实验室, 内蒙古呼和浩特 010018

通讯作者: 王明玖,E-mail:wangmj_0540@163.com
作者简介:田雨（1999-），女，汉族，内蒙古乌海人，硕士研究生，主要从事草种质资源与育种研究，E-mail：1580284783@qq.com
基金资助:
内蒙古草业技术创新中心有限公司重大创新平台建设专项“豆科牧草种子内生菌资源挖掘与利用（CCPTZX2023K01）”；内蒙古典型矿区土壤和植被修复重建及保护关键技术研究（2020ZD0020-3）资助

Abstract

Abstract: In order to investigate the effects of inoculation soybean rhizobia at different nitrogen levels on the growth and physiology of hybrid soybeans （Glycine soja Sieb.et Zucc）， in this study ‘Mengnong S006 hybrid forage soybean’ （Glycine soja Sieb.et Zucc cv. Hybrid F-S006）， referred to as S006， was planted with vermiculite， and four nitrogen levels CK（0 g·kg^-1）， N₁（0.714 g·kg^-1）， N₂（1.428 g·kg^-1） and N₃ （2.142 g·kg^-1） were set up. Three strains of rhizobia isolated from the root nodules of S006 in the experimental planting plot were respectively inoculated and named （Z₁， Z₂， Z₃）. The effects of rhizobia inoculation at different nitrogen levels on plant height， nodules， leaf length， leaf width， biomass， photosynthetic indexes， soluble sugar content， catalase content and other indexes of S006 were studied. Thirty days after inoculation with rhizobia， the growth indexes and physiological indexes of S006 were measured. The results showed that the application of nitrogen at N₂ level could significantly increase the photosynthesis and biomass of S006， and the physiological effect was more significant under the treatment of Rhizobia Z₁.

Key words: Hybrid forage soybean, Rhizobia, Nitrogen application, Biomass

摘要： 为研究在不同氮素水平下接种大豆根瘤菌对杂交饲用大豆（Glycine soja Sieb.et Zucc）生长和生理的影响，以‘蒙农S006饲用大豆’（Glycine soja Sieb.et Zucc cv. Hybrid F-S006），简称S006，为试验材料，采用蛭石种植，设置4个氮素水平CK（0 g·kg^-1），N₁（0.714 g·kg^-1），N₂（1.428 g·kg^-1），N₃（2.142 g·kg^-1），分别接种3株分离自试验地种植小区中S006根部根瘤的根瘤菌命名为（Z₁，Z₂，Z₃），研究在不同氮素水平下接种根瘤菌对S006株高、根瘤、叶长、叶宽、生物量、光合指标、可溶性糖含量、过氧化氢酶含量等指标的影响。接种根瘤菌后30 d后，对S006生长指标和生理指标进行测定。结果显示，在施用氮素为N₂水平下，能显著增加S006的光合作用和生物量，且在根瘤菌Z₁的处理下，对生理的影响较为显著。

关键词: 杂交饲用大豆, 根瘤菌, 施氮, 生物量

CLC Number:

S542

TIAN Yu, WANG Ming-jiu, WANG Zheng-qing, LI Shu-ning, ZHAO Wei-xuan, JIA Yun-peng. Effects of Rhizobial Inoculation on Growth and Physiology of Forage Soybean at Different Nitrogen Levels[J]. Acta Agrestia Sinica, 2025, 33(11): 3847-3858.

田雨, 王明玖, 王正卿, 李舒宁, 赵伟璇, 贾云鹏. 不同氮素水平下接种根瘤菌对饲用大豆生长和生理的影响[J]. 草地学报, 2025, 33(11): 3847-3858.

References

[1] WANG W W，HU Z H. Characteristics related to symbiotic nitrogen fixation of legumes in northwest arid zone of China[J]. Acta Botanica Boreali-occidentalia Sinica，2003，23（7）：107-112 王卫卫，胡正海. 几种生态因素对西北干旱地区豆科植物结瘤固氮的影响[J]. 西北植物学报，2003，23（7）：107-112
[2] LI Q，LUO Y H，YU D H，et al. Effects of low nitrogen stress on photosynthetic characteristics and chlorophyll fluorescence parameters of maize cultivars tolerant to low nitrogen stress at the seedling stage[J]. Journal of Plant Nutrition and Fertilizer，2015，21（5）：1132-1141 李强，罗延宏，余东海，等. 低氮胁迫对耐低氮玉米品种苗期光合及叶绿素荧光特性的影响[J]. 植物营养与肥料学报，2015，21（5）：1132-1141
[3] ZHOU X J，LIANG Y，CHEN H，et al. Effects of rhizobia inoculation and nitrogen fertilization on photosynthetic physiology of soybean[J]. Photosynthetica，2006，44（4）：530-535
[4] JIAO W J，MIN Q W，LIN K，et al. Progress and perspective on nutrition diagnosis of plant nitrogen[J]. Chinese Agricultural Science Bulletin，2006，22（12）：351-355 焦雯珺，闵庆文，林焜，等. 植物氮素营养诊断的进展与展望[J]. 中国农学通报，2006，22（12）：351-355
[5] 李俊，沈德龙，林先贵. 农业微生物研究与产业化进展[M]. 北京：科学出版社，2011
[6] XU Y X，XU Y，LI L，et al. Effects of rhizobia inoculation on nodulation and growth of spring soybean in southern Xinjiang[J]. Soybean Science，2021，40（1）：98-105 胥雅馨，徐玥，李玲，等. 接种根瘤菌对南疆春大豆结瘤和生长的影响[J]. 大豆科学，2021，40（1）：98-105
[7] HE C M，GAO H，WANG H J，et al. GSK3-mediated stress signaling inhibits legume-Rhizobium symbiosis by phosphorylating GmNSP1 in soybean[J]. Molecular Plant，2021，14（3）：488-502
[8] WEI Q S，GUO D S，WANG L，et al. Impacts of rhizobia inoculating on root nodule and yield of vegetable soybean under different fertilizer treatments[J]. Jiangsu Agricultural Sciences，2021，49（23）：77-82 魏启舜，郭东森，王琳，等. 不同施肥条件下接种根瘤菌对鲜食大豆结瘤和产量的影响[J]. 江苏农业科学，2021，49（23）：77-82
[9] YU S S. Effects of nitrogen application on flag leaf senescence and yield and quality formation of high-yield wheat[D]. Yantai：Yantai University，2024：1-3 余双双. 施氮水平对高产小麦旗叶衰老及产量、品质形成的影响[D]. 烟台：烟台大学，2024：1-3
[10] NIELSEN D C. Forage soybean yield and quality response to water use[J]. Field Crops Research，2011，124（3）：400-407
[11] GANDRA J R，TAKIYA C S，DEL VALLE T A，et al. Soybean whole-plant ensiled with chitosan and lactic acid bacteria： microorganism counts，fermentative profile，and total losses[J]. Journal of Dairy Science，2018，101（9）：7871-7880
[12] WANG N. Study on characteristics of new lines of wild soybean and cultivated soybean[D]. Hohhot：Inner Mongolia Agricultural University，2019. ：1-3 王娜. 野生大豆与栽培大豆杂交新品系特征特性研究[D]. 呼和浩特：内蒙古农业大学，2019. ：1-3
[13] LIU X J. Evaluation and utilization of excellent germplasm of the strains from the cross of Glycine soja and Glycine max[D]. Hohhot：Inner Mongolia Agricultural University，2017：2-8 刘雪骄. 栽培大豆与野生大豆杂交后代优异种质评价与利用[D]. 呼和浩特：内蒙古农业大学，2017：2-8
[14] LI S N，WANG M J，DU Y Q，et al. Biological function and symbiotic matching of rhizobia in two hybrid forage soybean[J]. Soybean Science，2024，43（1）：35-44 李舒宁，王明玖，杜雨芊，等. 两个杂交饲用大豆的根瘤菌生物功能与共生匹配性[J]. 大豆科学，2024，43（1）：35-44
[15] WU H. Isolation、Indentification and application of elite soybean rhizobia strains[D]. Wuhan：Huazhong Agricultural University，2017：16-17 伍惠. 优良大豆根瘤菌株的分离、鉴定及应用研究[D]. 武汉：华中农业大学，2017：16-17
[16] LIU J W. Rhizobium diversity and nodulation and nitrogen fixation mechanism in Trifolium ambiguum[D]. Hohhot：Inner Mongolia Agricultural University，2022：17-20 刘嘉伟. 高加索三叶草根瘤菌多样性及结瘤固氮机理研究[D]. 呼和浩特：内蒙古农业大学，2022：17-20
[17] ZHANG X H，MA S Y，MA L，et al. Effects of Rhizobium inoculation on the growth and photosynthetic characteristics of pea plants[J]. Acta Agrestia Sinica，2021，29（6）：1234-1241 张旭辉，马绍英，马蕾，等. 接种根瘤菌对重茬豌豆植株生长及光合特性的影响[J]. 草地学报，2021，29（6）：1234-1241
[18] LIANG F Q，GUAN D W，DANG B L，et al. Effect of Rhizobium and nitrogen on plant characteristics and yield of soybean[J]. Heilongjiang Agricultural Sciences，2017（8）：28-31 梁福琴，关大伟，党蓓蕾，等. 根瘤菌和氮素对大豆植株特性及产量的影响[J]. 黑龙江农业科学，2017（8）：28-31
[19] JI Y M，LUO R P，ZHAO Z G，et al. Effects of different nitrogen application and methods on nodule growth and yield of soybean （Glycine max L.）[J]. Soybean Science，2017，36（6）：887-893 姬月梅，罗瑞萍，赵志刚，等. 不同施氮量及施氮方式对大豆根瘤生长及产量的影响[J]. 大豆科学，2017，36（6）：887-893
[20] MA X，WANG L L，LI W J，et al. Effects of different nitrogen levels on nitrogen fixation and seed production of alfalfa inoculated with rhizobia[J]. Acta Prataculturae Sinica，2013，22（1）：95-102 马霞，王丽丽，李卫军，等. 不同施氮水平下接种根瘤菌对苜蓿固氮效能及种子生产的影响[J]. 草业学报，2013，22（1）：95-102
[21] WEI H，GUO J，ZHOU Q，et al. Studies on biological-organic-inorganic fertilizer and its field application[J]. Journal of Microbiology，1997，17（3）：18-24 魏辉，郭俊，周强，等. 生物有机无机复合肥的研制与效果研究[J]. 微生物学杂志，1997，17（3）：18-24
[22] WANG S Q，HAN X Z，QIAO Y F，et al. Root morphology and nitrogen accumulation in soybean （Glycine max L.） under different nitrogen application levels[J]. Chinese Journal of Eco-Agriculture，2009，17（6）：1069-1073 王树起，韩晓增，乔云发，等. 施氮对大豆根系形态和氮素吸收积累的影响[J]. 中国生态农业学报，2009，17（6）：1069-1073
[23] GENG Y Y，WANG G R，ZHANG Y F，et al. Effects of N and P on root nodules growth and nitrogen fixation of Glycyrrhiza uralensis Fisch.treated with Rhizobium solution[J]. Central South Pharmacy，2022，20（5）：1089-1094 耿业业，王桂荣，张远帆，等. 氮、磷对根瘤菌液处理下甘草根瘤生长及固氮活力的影响[J]. 中南药学，2022，20（5）：1089-1094
[24] ZUFFO A M，RATKE R F，OKLA M K，et al. Understanding the contribution of soybean crop residues inoculated with Bradyrhizobium spp. and not harvested on nitrogen supply in off-season corn cultivars[J]. PLoS One，2022，17（6）：e0269799
[25] NOMURA K，ITO M，KUSABA Y，et al. Estimation of the optimal leaf area index （LAI） of an eggplant canopy based on the relationship between the nighttime respiration and daytime photosynthesis of the lowermost leaves[J]. Scientia Horticulturae，2023，307：111525
[26] ZHANG M C. Effects of starter-N： plus topdressing N on[D]. Harbin：Northeast Agricultural University，2013：77-78 张明聪. 启动氮加追氮对不同密度大豆光合生产能力的影响[D]. 哈尔滨：东北农业大学，2013：77-78
[27] XU Y X. Effects of nitrogen application and inoculation with rhizobia on soybean nodule formation and growth in southern Xinjiang[D]. Ala’er：Tarim University，2021：70-72 胥雅馨. 施氮及根瘤菌拌种对南疆大豆结瘤和生长的影响[D]. 阿拉尔：塔里木大学，2021：70-72
[28] CRAWFORD N M. Nitrate： nutrient and signal for plant growth[J]. The Plant Cell，1995，7（7）：859-868
[29] LI H H，LIU H，PANG J，et al. Effects of water and nitrogen interaction on growth and nutrient accumulation of potted tomatoes[J]. Transactions of the Chinese Society for Agricultural Machinery，2019，50（9）：272-279 李欢欢，刘浩，庞婕，等. 水氮互作对盆栽番茄生长发育和养分累积的影响[J]. 农业机械学报，2019，50（9）：272-279
[30] XIAO N H，LI Z Y. Effect of nitrogen application at seedling stage on growth and yield of soybean[J]. Chinese Journal of Oil Crop Sciences，1982，4（4）：42-46 肖能遑，李志玉. 苗期施氮对大豆生长发育及产量的影响[J]. 中国油料，1982，4（4）：42-46
[31] DING X G，LIU Y J，ZHANG X S，et al. Effects of different concentrations of exponential fertilization on the growth of Chorisia speciosa and other two tree species seedlings[J]. Ecology and Environmental Sciences，2013，22（4）：619-624 丁晓纲，刘喻娟，张晓珊，等. 不同浓度指数施肥对美丽异木棉等3个树种幼苗生长的影响[J]. 生态环境学报，2013，22（4）：619-624
[32] ZHOU X J. Effects of rhizobia，NH₄～+-fertilizer and light strength on nitrogen fixation and photosynthesis of soybean[D]. Beijing：Institute of Botany，Chinese Academy of Sciences，2006：50-53 周相娟. 根瘤菌、铵态氮、光照强度对大豆固氮和光合作用影响的研究[D]. 北京：中国科学院研究生院（植物研究所），2006：50-53
[33] ZHONG Z M，YANG Q，WENG B Q，et al. Analysis of the relationship between fertilizer application and growth performance of two Chamaecrista spp in hilly area of Southern China[J]. Acta Agrestia Sinica，2023，31（10）：3085-3093 钟珍梅，杨庆，翁伯琦，等. 南方丘陵区施肥量与2种决明生长性能关系分析[J]. 草地学报，2023，31（10）：3085-3093
[34] SHU X，JIN M H，WANG S Y，et al. The effect of nitrogen and potassium interaction on the leaf physiological characteristics，yield，and quality of sweet potato[J]. Agronomy，2024，14（10）：2319
[35] THOMPSON J B，SLOT M，DALLING J W，et al. Species-specific effects of phosphorus addition on tropical tree seedling response to elevated CO₂[J]. Functional Ecology，2019，33（10）：1871-1881
[36] ZONG Y Z，ZHANG H Q，LI P，et al. Leaf nitrogen have a better relationship with photosynthesis performance across wheat species under elevated CO₂ and drought[J]. Plant Physiology and Biochemistry，2021，166：964-973
[37] ZHU T Q，LIU X J，HAO F. Photosynthetic responses of alfalfa leaves to nitrogen[J]. Grassland and Turf，2017，37（6）：31-35，41 朱天琦，刘晓静，郝凤. 2个紫花苜蓿品种叶片对氮的光合响应[J]. 草原与草坪，2017，37（6）：31-35，41
[38] SUN Q S，YUAN C，ZHANG Y X. Effects of reducing nitrogen fertilizer and inoculating Rhizobium on photosynthetic characteristics and yield of black soybean[J]. Crops，2022（4）：132-137 孙庆圣，原程，张玉先. 减施氮肥和接种根瘤菌对黑大豆光合特性及产量的影响[J]. 作物杂志，2022（4）：132-137
[39] LIAO L N. Effects of nitrogen application and inoculation on growth，physiological characteristics and nitrogen utilization in Eucalyptus urophylla×e.grandis-Dalbergia odorifera intercropping systems[D]. Nanning：Guangxi University，2020：48-50 廖良宁. 施氮与接种固氮菌对尾巨桉-降香黄檀混作幼苗生长、生理及氮素利用的影响[D]. 南宁：广西大学，2020：48-50
[40] ZHANG Y Y，HU D D，MA C H，et al. Leaf structure and photosynthetic properties of alfalfa in response to bacteria and phosphorus addition[J]. Acta Prataculturae Sinica，2024，33（8）：133-144 张盈盈，胡丹丹，马春晖，等. 苜蓿叶片结构和光合特性对菌磷添加的响应[J]. 草业学报，2024，33（8）：133-144
[41] ARMENGAUD P，THIERY L，BUHOT N，et al. Transcriptional regulation of proline biosynthesis in Medicago truncatula reveals developmental and environmental specific features[J]. Physiologia Plantarum，2004，120（3）：442-450
[42] ZHAO J T，LI X F，LI H，et al. Research on the role of the soluble sugar in the regulation of physiological metabolism in higher plant[J]. Journal of Anhui Agricultural Sciences，2006，34（24）：6423-6425，6427 赵江涛，李晓峰，李航，等. 可溶性糖在高等植物代谢调节中的生理作用[J]. 安徽农业科学，2006，34（24）：6423-6425，6427
[43] WANG S L，GUO T C，WANG C Y，et al. Soluble sugar contents in leaf and grain in two gluten wheatsand its relationship with grain starch accumulation[J]. Journal of Henan Agricultural Sciences，2005，34（4）：12-15 王书丽，郭天财，王晨阳，等. 两种筋力型小麦叶、粒可溶性糖含量及与籽粒淀粉积累的关系[J]. 河南农业科学，2005，34（4）：12-15
[44] XU Y，LUO S G，LIU Y Y，et al. Effects of N and Mg application on growth and yield of soybean[J]. Journal of Northeast Agricultural University，2009，40（1）：49-53 徐洋，罗盛国，刘元英，等. 氮镁交互对大豆生长及产量的影响[J]. 东北农业大学学报，2009，40（1）：49-53
[45] HU X Q. Effects of optimized fertilization on N and soluble sugar distribution and yield of soybean[D]. Harbin：Northeast Agricultural University，2010：34-35 胡晓青. 优化施肥对大豆体内氮和可溶性糖积累及产量的影响[D]. 哈尔滨：东北农业大学，2010：34-35
[46] LUAN H H. Effects of combined application of probiotic bacteria with nitrogen and phosphorus on the growth and development of adzuki bean[D]. Linfen：Shanxi Normal University，2018：15-15 栾换换. 促生菌与氮和磷配施对红小豆生长发育的影响[D]. 临汾：山西师范大学，2018：15-15
[47] LI X. Effects of high temperature treatment at different stages after anthesis on premature senescence of spring wheat[D]. Yinchuan：Ningxia University，2021：46-47 李鑫. 花后不同时期高温处理对春小麦早衰的影响机制[D]. 银川：宁夏大学，2021：46-47
[48] TIAN W Z，FENG W S，LI J H，et al. Effects of mild drought in different periods on yield characters and antioxidant enzyme activities in flag leaves of wheat[J]. Jiangsu Agricultural Sciences，2021，49（22）：99-104 田文仲，冯伟森，李俊红，等. 不同时期轻度干旱对小麦产量性状及旗叶抗氧化酶活性的影响[J]. 江苏农业科学，2021，49（22）：99-104
[49] ZHAO R H，CHEN H W，OU X Q，et al. Effect of nitrogen levels on oxidase activities in leaves and roots of winter wheat[J]. Chinese Agricultural Science Bulletin，2020，36（11）：1-7 赵若含，陈红卫，欧行奇，等. 不同氮素水平对冬小麦根叶氧化酶活性的影响[J]. 中国农学通报，2020，36（11）：1-7
[50] WANG H Z，ZHANG J，WU J Z，et al. Effect of different levels of nitrogen on physiological characteristics of flag leaves and grain yield of wheat[J]. Acta Prataculturae Sinica，2013，22（4）：69-75 王贺正，张均，吴金芝，等. 不同氮素水平对小麦旗叶生理特性和产量的影响[J]. 草业学报，2013，22（4）：69-75
[51] MENG F F，GUAN H F，YANG H，et al. The effect of nitrogen and magnesium interaction on the growth and physiological characteristics of fir seedlings[J]. Journal of Fujian Agriculture and Forestry University （Natural Science Edition），2025，54（1）：36-46 孟芳芳，关恒锋，杨浩，等. 氮、镁交互作用对杉木幼苗生长和生理特性的影响[J]. 福建农林大学学报（自然科学版），2025，54（1）：36-46

Effects of Rhizobial Inoculation on Growth and Physiology of Forage Soybean at Different Nitrogen Levels

不同氮素水平下接种根瘤菌对饲用大豆生长和生理的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Lei, ZHANG Yu-juan, SUN Shi-xian, XU Xue-bao, XING Yue, LIU Xiao-li, GAO Cui-ping, WANG Cheng-jie. Effects of Different Grazing Intensities on Soil Organic Carbon Content in Desert Steppe [J]. Acta Agrestia Sinica, 2025, 33(8): 2541-2547.
[2]	DUAN Jun-guang, FANG Kai, PEI Lu, CHU Jian-min, ZHANG Jin-xin, LI Xiao-xia, WANG Ying-xin. Changes of Trade-offs Between Aboveground and Belowground Plant Biomass Across Different Grazing Intensities of Alpine Meadow [J]. Acta Agrestia Sinica, 2025, 33(8): 2575-2584.
[3]	LIU Chang, CHEN Ji-shan, ZHU Rui-fen, SUN Wan-bin, YAO Bo, DONG Shui-kui. Meta-analysis of the Impacts of Nitrogen Addition on Biomass and Soil Organic Carbon Content of China Grasslands [J]. Acta Agrestia Sinica, 2025, 33(7): 2078-2089.
[4]	ZHONG Hua, HAO Jie, LIANG Wen-jun, Gao Yang-yang, LI Lang, WANG Ya-nan, WANG Chang-hui, DONG Kuan-hu. Effects of Various Nitrogen Forms Addition on Grassland Biomass in the Agro-Pastoral Ecotone in Northern Shanxi [J]. Acta Agrestia Sinica, 2025, 33(7): 2198-2205.
[5]	WANG Sheng-ju, LIU Wen-hao, JIN Gui-li, CUI Guo-ying, ZHANG Yong-juan, WEI Xiu-hong, LI Chao, CHEN Meng-tian, LI Wen-xiong, DU Wen-lin. Temporal and Spatial Changes of Grassland Aboveground Biomass and Its Response to Climate Factors in Xinjiang [J]. Acta Agrestia Sinica, 2025, 33(7): 2320-2332.
[6]	YAO Jun-fei, WEI Guo-liang, WU Fa-liang, LI Xu-dong, Adihaze, SHI Zheng-chen, ZHANG Zhong-hua, MA Li, LI Shan, LI Hong-lin, LI Qiang-feng, WANG Zhen, ZHOU Hua-kun, ZHANG Qiang. Effects of Different Grazing Management Practices on the Contents of Soils and Microbiomass and Their Stoichiometric Characteristics in Alpine Grassland [J]. Acta Agrestia Sinica, 2025, 33(6): 1749-1755.
[7]	LI Yan, XU Jing-hang, WU Jian-ping. Effects of Biochar Addition on Plant Biomass in A Degraded Cultivated Grassland in Zhaotong， Yunnan Province [J]. Acta Agrestia Sinica, 2025, 33(6): 1991-2001.
[8]	XU Ai-yun, QIN Yi-tong, ZHANG Yuan-hong, LI Tian, CAO Bing. Effects of Elevated CO₂ Concentration on the Growth of Agropyron mongolicum Under Short-Term Nitrogen Addition Scenario [J]. Acta Agrestia Sinica, 2025, 33(4): 1211-1217.
[9]	LI Wen-xiong, JIN Gui-li, LIU Wen-hao, LI Jia-xin, WANG Sheng-ju, CHEN Meng-tian, LI Chao, DU Wen-lin, Davuletti Khaled Samugal, Serik Eli Yeridana. Research on Aboveground Biomass Inversion of Desert Grassland Based on UAV Remote Sensing [J]. Acta Agrestia Sinica, 2025, 33(4): 1258-1266.
[10]	HAO Jie, NIU Hui-min, WANG Chang-hui, DIAO Hua-jie, WU Shuai-kai, SU Yuan, GAO Yang-yang, LIANG Wen-jun, YANG Xiu-yun, DONG Kuan-hu. Effects of Nutrient Addition and Shallow Plowing on the Biomass of Grasslands in the Agro-pastoral Ecotone of Northern Shanxi [J]. Acta Agrestia Sinica, 2025, 33(4): 1299-1307.
[11]	ALIMIRI-Alimujiang, TANG Bang-jie, KONG Fan-xi, CUI Wen-kai, CHEN Jun. Study on Convenient Estimation Technique of Aboveground Biomass of Natural Grassland in Xinjiang [J]. Acta Agrestia Sinica, 2025, 33(4): 1308-1315.
[12]	AN Hai-tao, SUN Cai-cai, DONG Quan-min, YANG Xiao-xia, LIU Wen-ting, WANG Xin-xin, WANG Xiao-li, ZHAO Xin-quan. Responses of Rhizosphere Soil and Bulk Soils Microbial Activity to Livestock Assembly [J]. Acta Agrestia Sinica, 2025, 33(3): 813-822.
[13]	CAI Hai, SHI Qian-qian, WANG Ting, DU Yan-gong. Impact of Fencing on the Characteristics of the Temperate Desert Vegetation Community in the Qinghai Lake Basin [J]. Acta Agrestia Sinica, 2025, 33(3): 929-935.
[14]	CHEN Wei-hao, ZHANG Guo-rui, XU Yu-dan, YANG Wei-ke, LI Jia-cheng, LI Shuai, YAN Shi-jie, ZHAO Xiang. Effects of Disturbance on Plants, Soils, and Microorganisms in Subalpine Meadows and Frost Mounds [J]. Acta Agrestia Sinica, 2025, 33(1): 107-118.
[15]	LYU Wei-dong, DONG Quan-min, SUN Cai-cai, LIU Wen-ting, FENG Bin, LIU Yu-zhen, ZHANG Zhen-xiang, YANG Xiao-xia. Effects of Warm-Season Grazing by Yak and Tibetan Sheep on Different Fractions of Carbon and Nitrogen in Alpine Grassland of the Qinghai-Xizang Plateau [J]. Acta Agrestia Sinica, 2025, 33(1): 125-135.