Genome-wide Identification of Glutathione S-Transferases （GSTs） Gene Family and Its Responses to Cadmium Stress in Ramie （Boehmeria nivea）

doi:10.11733/j.issn.1007-0435.2026.01.002

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 10-22.DOI: 10.11733/j.issn.1007-0435.2026.01.002

Genome-wide Identification of Glutathione S-Transferases （GSTs） Gene Family and Its Responses to Cadmium Stress in Ramie （Boehmeria nivea）

YAN Shi-wei¹, GU Yi-zhou¹, ZHAO Hao-han¹, LIANG Shu-yue¹, GAO Gang¹, CHEN Ping¹, ZHU Ai-guo^1,2,3, CHEN Ji-kang^1,2,3

1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan Province 410221, China;
2. Key laboratory of Genetic Breeding and Microbial Processing for Bast Fiber Crops, MARA, Changsha, Hunan Province 410221, China;
3. Yuelushan Laboratory, Changsha, Hunan Province 410128, China

Received:2025-03-17 Revised:2025-06-12 Published:2025-12-24

苎麻谷胱甘肽S-转移酶家族全基因组鉴定及其对镉胁迫的响应

颜世伟¹, 顾一舟¹, 赵浩含¹, 梁舒月¹, 高钢¹, 陈平¹, 朱爱国^1,2,3, 陈继康^1,2,3

1. 中国农业科学院麻类研究所, 湖南长沙 410221;
2. 农业农村部麻类生物学与生物加工重点实验室, 湖南长沙 410221;
3. 岳麓山实验室, 湖南长沙 410128

通讯作者: 陈继康,E-mail:chenjikang@caas.cn
作者简介:颜世伟（1997-），男，汉族，湖南衡阳人，硕士研究生，主要从事苎麻抗逆遗传育种与利用研究，E-mail:15273496700@163.com；
基金资助:
湖南省自然科学基金（2024JJ7622）；国家麻类产业技术体系（CARS-16）；湖南省湖湘青年英才计划（2022RC1013）资助

Abstract

Abstract: Glutathione S-transferases （GSTs） play an important role in the regulation of plants against stressful environments， but the systematic studies on Ramie were lack. In this paper， a total of 43 BnGSTs gene families were identified by bioinformatic analyses， most of which were predicted to be located in the cytoplasm and belonged to the plant-specific Tau and Phi class. Inter-species covariance analysis revealed that BnGSTs genes represented more orthologous genes in dicots than monocots； intra-species covariance analysis revealed that there were five pairs of paralogous genes among BnGSTs， all of which were derived from the whole genome or fragmentary gene duplication， and all of them were found that Ka/Ks ratios were less than 1， indicating that they were subjected to purifying selection. Cis-acting element prediction revealed that the upstream of these BnGSTs genes was rich in light-responsive， phytohormone-regulated and stress-responsive elements combined with transcriptome expression data， nine tissue-specific candidate genes were screened with significant （P<0.05） changes on expression patterns in roots， stems and leaves under cadmium stress. Two genes， BnGST22 and BnGST12， were identified as having significant up-regulation and high relative expression， which could be used as target genes for further functional verification. This study further enriched the functional gene pool of ramie and provided a theoretical basis for the use of anti-stress resources and variety selection.

Key words: Ramie, Glutathione S-transferases, Cadmium stress, Bioinformatic analysis

摘要： 谷胱甘肽S-转移酶（Glutathione S-Transferase，GSTs）在调控作物抵御胁迫环境中发挥着重要作用，但当前缺乏针对苎麻（Boehmeria nivea）的系统研究。本文通过生物信息分析，鉴定出43个苎麻GSTs家族成员，预测其大部分定位于细胞质中，归属于植物特有的Tau和Phi类；物种间共线性发现BnGSTs基因同双子叶植物间对比单子叶植物存在更多直系同源基因，物种内共线性发现BnGSTs成员中有5对旁系同源基因，来源于全基因组或片段复制，且Ka/Ks值均小于1，表明其受到纯化选择；顺式元件预测发现这些BnGSTs成员基因上游富含光反应、植物激素调控和胁迫反应元件，结合转录组数据筛选出的9个组织特异性表达候选基因，其在镉胁迫条件下出现显著变化（P<0.05），且在根茎叶中呈现不同的表达模式，鉴定到BnGST22，BnGST12等2个基因上调变化显著且相对表达量较高，可作为目的基因进行进一步功能验证。本研究进一步丰富了苎麻抗逆功能基因库，为抗逆资源利用和品种选育提供了理论依据。

关键词: 苎麻, 谷胱甘肽S-转移酶, 镉胁迫, 生物信息分析

CLC Number:

S813.3

YAN Shi-wei, GU Yi-zhou, ZHAO Hao-han, LIANG Shu-yue, GAO Gang, CHEN Ping, ZHU Ai-guo, CHEN Ji-kang. Genome-wide Identification of Glutathione S-Transferases （GSTs） Gene Family and Its Responses to Cadmium Stress in Ramie （Boehmeria nivea）[J]. Acta Agrestia Sinica, 2026, 34(1): 10-22.

颜世伟, 顾一舟, 赵浩含, 梁舒月, 高钢, 陈平, 朱爱国, 陈继康. 苎麻谷胱甘肽S-转移酶家族全基因组鉴定及其对镉胁迫的响应[J]. 草地学报, 2026, 34(1): 10-22.

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Genome-wide Identification of Glutathione S-Transferases （GSTs） Gene Family and Its Responses to Cadmium Stress in Ramie （Boehmeria nivea）

苎麻谷胱甘肽S-转移酶家族全基因组鉴定及其对镉胁迫的响应

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