Abstract: CCCH zinc finger proteins,regulatory factors in various species,are crucial for plant growth,development,stress response,and other bio-processes. This study identified 59 CCCH gene families from the Ramie genome using bioinformatics methods. These genes were then analyzed for physicochemical properties,phylogeny,gene structure,colinearity analysis,and expression patterns. The analyses showed that these CCCH genes were unevenly distributed across the 14 chromosomes of Ramie. The cis-acting element prediction analyses found a large number of abiotic stress and hormone-responsive elements in the promoter region. Expression profiling showed that the differential genes BniC3H48,BniC3H53, and BniC3H57 were upregulated when the plant was sprayed with a mixture of ethylene glycol and potassium dihydrogen phosphate. These three genes may play an important role in the regulation of Cd enrichment in ramie through the interaction of ethylene glycol and potassium dihydrogen phosphate. The present study provides new clues and a theoretical basis for further analysis of the molecular mechanism of ramie in response to heavy metal cadmium stress.

Key words: Ramie, CCCH, Gene family, Expression pattern

摘要： CCCH锌指蛋白作为各种生物体中广泛存在的调节因子,在植物生长发育,胁迫应答等多种生物过程中发挥重要的调控作用。本研究采用生物信息学方法,从苎麻基因组中鉴定出59个CCCH基因家族成员,并进行了理化性质、系统进化、基因结构、共线性及表达模式的分析。结果表明CCCH基因不均匀分布在苎麻的14条染色体上;顺式作用元件预测分析发现启动子区有大量非生物胁迫和激素响应元件;表达谱分析表明,差异基因BniC3H48、BniC3H53和BniC3H57在乙烯利和磷酸二氢钾两者混合喷施后表达呈上调趋势,这三个基因可能在乙烯利和磷酸二氢钾互作调控苎麻富集镉中发挥重要作用。本研究为进一步解析苎麻响应重金属镉胁迫的分子机制提供新的线索和理论依据。

关键词: 苎麻, CCCH, 基因家族, 表达模式