The Alimiating Effects of Low-Temperature Stress on Egg Diapause Termination and Transcriptome Analysis in Ceracris kiangsu Tsai

doi:10.11733/j.issn.1007-0435.2026.01.008

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 75-86.DOI: 10.11733/j.issn.1007-0435.2026.01.008

The Alimiating Effects of Low-Temperature Stress on Egg Diapause Termination and Transcriptome Analysis in Ceracris kiangsu Tsai

FAN Yu-yang¹, GU Wen-jun¹, CHEN Peng², CHEN Xin-qiang³, ZHANG Rui⁴, GAO Yan¹, ZHAO Han-yang¹, PENG Zhong-gu¹, WEN Yi-fu¹

1. Yunnan Agricultural University, Kunming, Yunnan Province 650500, China;
2. Institute of Forest Protection, Yunnan Academy of Forestry and Grassland Science, Kunming, Yunnan Province 650201, China;
3. Yinlin Branch of Yunnan Forest Inventory and Planning Institute, Institute, Kunming, Yunnan Province 650021, China;
4. Kunming Insect Hunting Agricultural Technology Co., Ltd. Kunming, Yunnan Province 650599, China

Received:2025-06-19 Revised:2025-10-16 Published:2025-12-24

低温胁迫对黄脊竹蝗卵滞育的消除影响及其转录组分析

范宇阳¹, 谷汶骏¹, 陈鹏², 陈鑫强³, 张锐⁴, 高俨¹, 赵含洋¹, 彭仲谷¹, 文亦芾¹

1. 云南农业大学, 云南昆明 650500;
2. 云南省林业和草原科学院森林保护研究所, 云南昆明 650201;
3. 云南省林业调查规划院营林分院, 云南昆明 650021;
4. 昆明猎虫农业科技有限公司, 云南昆明 650500

通讯作者: 文亦芾,E-mail:1301814839@qq.com
作者简介:范宇阳（2001-），女，汉族，本科，山西长治人，硕士研究生，主要从事草原生态与保护研究，E-mail:1006047792@qq.com；
基金资助:
云南省科技厅重大科技专项（202502AE090016）资助

Abstract

Abstract: Ceracris kiangsu Tsai is a major pest seriously threatens forestry and grassland ecosystems， with its egg diapause being a critical factor for overwinter survival and population outbreaks. In this study we investigated the molecular response mechanisms of egg diapause release in C. kiangsu by applying different low temperatures （3℃ and 8℃） to the diapausing eggs and combining with transcriptome sequencing. The results showed that the diapause release effect was closely related to the duration and temperature of low-temperature treatment， with a 60-day period being a critical turning point for shortened hatching and increased hatching rates. Hatching rates were significantly higher at 8℃ compared to 3℃. Transcriptome analysis revealed that compared to the 25℃ control group， 3℃ and 8℃ treatments identified 9730 and 6075 differentially expressed genes （FDR < 0.05， log₂FC > 1）， respectively. GO and KEGG enrichment analyses revealed that these genes were significantly enriched in metabolic processes， including arginine metabolism， proline metabolism， and insect hormone biosynthesis， and stress responses， including FOXO signaling pathway and MAPK signaling pathway. Key genes such as ADAMTSL4， ALDH16A1， and foxo were identified， which regulate metabolic processes and developmental progression under low-temperature stress. These findings demonstrate novel insights into the molecular mechanisms of diapause release and highlight the potential for developing new green control targets for managing this pest.

Key words: Ceracris kiangsu Tsai, Egg diapause, Embryonic development, Transcriptome

摘要： 黄脊竹蝗（Ceracris kiangsu Tsai）是严重威胁林草业的重大害虫，其卵滞育特性对越冬存活及种群爆发至关重要。本研究通过设置不同低温（3℃和8℃）处理滞育卵，结合转录组测序技术，系统探究其滞育解除的分子响应机制。结果表明：滞育解除效果与低温时长和温度密切相关，60天是孵化期缩短和孵化率上升的关键转折点，且8℃处理的孵化率显著高于3℃处理。转录组分析显示，与25℃对照相比，3℃和8℃处理分别鉴定出9730个和6075个差异表达基因（FDR<0.05，log₂FC>1）。GO与KEGG富集分析揭示，差异基因显著富集于代谢过程、应激反应及昆虫激素生物合成等通路。进一步筛选出ADAMTSL4，ALDH16A1，foxo等9个关键基因，其参与低温胁迫下滞育卵的代谢调节与发育进程。本研究筛选到的差异基因和代谢通路，为解析害虫越冬适应性及开发绿色防控新靶点提供了重要理论依据。

关键词: 黄脊竹蝗, 卵滞育, 胚胎发育, 转录组

CLC Number:

Q968.1
Q963

FAN Yu-yang, GU Wen-jun, CHEN Peng, CHEN Xin-qiang, ZHANG Rui, GAO Yan, ZHAO Han-yang, PENG Zhong-gu, WEN Yi-fu. The Alimiating Effects of Low-Temperature Stress on Egg Diapause Termination and Transcriptome Analysis in Ceracris kiangsu Tsai[J]. Acta Agrestia Sinica, 2026, 34(1): 75-86.

范宇阳, 谷汶骏, 陈鹏, 陈鑫强, 张锐, 高俨, 赵含洋, 彭仲谷, 文亦芾. 低温胁迫对黄脊竹蝗卵滞育的消除影响及其转录组分析[J]. 草地学报, 2026, 34(1): 75-86.

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The Alimiating Effects of Low-Temperature Stress on Egg Diapause Termination and Transcriptome Analysis in Ceracris kiangsu Tsai

低温胁迫对黄脊竹蝗卵滞育的消除影响及其转录组分析

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