Effects of Sucrose and Lactic Acid Bacteria Inoculantion on Dominant Clostridia and Lactic Acid Bacteria Communities in High-moisture Alfalfa Silage

doi:10.11733/j.issn.1007-0435.2026.01.034

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 356-365.DOI: 10.11733/j.issn.1007-0435.2026.01.034

Effects of Sucrose and Lactic Acid Bacteria Inoculantion on Dominant Clostridia and Lactic Acid Bacteria Communities in High-moisture Alfalfa Silage

LI Qin^1,2, LIN Bing¹, MAO Pei-chun², TIAN Xiao-xia², MA Shuai², MENG Lin², ZHENG Ming-li², GUO Yu-xia¹

1. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan Province 450046, China;
2. Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2025-03-18 Revised:2025-05-23 Published:2025-12-24

添加蔗糖和乳酸菌剂对高水分苜蓿青贮中优势梭菌和乳酸菌菌群的影响

李钦^1,2, 林冰¹, 毛培春², 田小霞², 马帅², 孟林², 郑明利², 郭玉霞¹

1. 河南农业大学动物科技学院, 河南郑州 450046;
2. 北京市农林科学院草业花卉与景观生态研究所, 北京 100097

通讯作者: 郑明利,E-mail:bjzhengml@163.com;郭玉霞,E-mail:yuxiaguo@163.com
作者简介:李钦（2000-），男，汉族，河南新乡人，硕士研究生，主要从事动物营养与饲料科学研究，E-mail:19503820416@163.com；
基金资助:
国家自然科学基金项目（32201461）；北京市农林科学院青年科研基金项目（QNJJ202310）资助

Abstract

Abstract: The aim of this study was to investigate the diversity of dominant Clostridia community and screen targeted bacteriostatic lactic acid bacteria in high-moisture alfalfa silage through high-throughput sequencing combined with correlation network analyses. Sucrose group （sucrose added at 20 g·kg^-1 fresh matter， FM）， LAB group （LAB inoculant was composed of Lactiplantibacillus plantarum and Lentilactobacillus buchneri and applied at 10⁶ cfu·g^-1 FM）， and control group were established. All groups were subjected to 56 days of anaerobic ensiling at ambient temperature. The results showed that severe butyric acid fermentation occurred in both the control and LAB groups. Compared to the control and LAB groups， the sucrose group significantly increased the lactic acid content （P<0.05）， while significantly decreased butyric acid， ammonia nitrogen， and Clostridia counts （P<0.05）. Clostridium sp. BTY5， Garciella sp. GK3， and Clostridiales bacterium mt11 were significantly positively correlated with butyric acid， ammonia nitrogen， and Clostridia counts （P<0.05）. L. buchneri and Lactobacillus melliventris were significantly negatively correlated with these three key Clostridia （P<0.05） and were significantly negatively correlated with pH， butyric acid， ammonia nitrogen， and Clostridia counts （P<0.05）. In conclusion， Clostridium sp. BTY5， Garciella sp. GK3， and Clostridiales bacterium mt11 were identified as the key Clostridia responsible for butyric acid fermentation in high-moisture alfalfa silage. The addition of sucrose can effectively improve silage fermentation quality. L. buchneri and L. melliventris have the potential to inhibit targeted key Clostridia.

Key words: Alfalfa silage, Clostridia, Lactic acid bacteria, Fermentation quality

摘要： 试验旨在通过高通量测序技术并结合相关性网络分析探讨高水分苜蓿青贮中优势梭菌菌群多样性并筛选靶向抑菌乳酸菌。设置蔗糖组（添加量为20 g·kg^-1 FM）、乳酸菌组（Lactiplantibacillus plantarum和Lentilactobacillus buchneri组成，接种量为10⁶ cfu·g^-1 FM）和对照组，室温厌氧发酵56 d。结果表明：对照组和乳酸菌组均发生严重的丁酸发酵；与对照组和乳酸菌组相比，蔗糖组显著提高了苜蓿青贮的乳酸含量（P<0.05），显著降低了丁酸含量、氨态氮含量和梭菌数量（P<0.05）。Clostridium sp. BTY5、Garciella sp.GK3和Clostridiales bacterium mt11与丁酸、氨态氮以及梭菌数量呈显著正相关（P<0.05）。L. buchneri和Lactobacillus melliventris与这3种关键梭菌呈显著负相关（P<0.05），并且与pH值、丁酸、氨态氮及梭菌数量呈显著负相关（P<0.05）。综上所述，Clostridium sp. BTY5、Garciella sp. GK3和Clostridiales bacterium mt11是引起高水分苜蓿青贮丁酸发酵的关键梭菌，添加蔗糖可有效改善青贮发酵品质，L. buchneri和L. melliventris具有靶向抑制关键梭菌的潜力。

关键词: 苜蓿青贮, 梭菌, 乳酸菌, 发酵品质

CLC Number:

S153

LI Qin, LIN Bing, MAO Pei-chun, TIAN Xiao-xia, MA Shuai, MENG Lin, ZHENG Ming-li, GUO Yu-xia. Effects of Sucrose and Lactic Acid Bacteria Inoculantion on Dominant Clostridia and Lactic Acid Bacteria Communities in High-moisture Alfalfa Silage[J]. Acta Agrestia Sinica, 2026, 34(1): 356-365.

李钦, 林冰, 毛培春, 田小霞, 马帅, 孟林, 郑明利, 郭玉霞. 添加蔗糖和乳酸菌剂对高水分苜蓿青贮中优势梭菌和乳酸菌菌群的影响[J]. 草地学报, 2026, 34(1): 356-365.

References

[1] YANG F Y. New concept “forage is a part of grains” to utmost spurring the development of forage industry[J]. Agrestia Sinica，2023，31（2）：311-313 杨富裕. 树立“饲草就是粮食”理念，大力发展饲草产业[J]. 草地学报，2023，31（2）：311-313
[2] LU X S. The quality and quantity issues of alfalfa products development[J]. China Dairy，2021（8）：9-12 卢欣石. 苜蓿饲草产业发展的质与量问题[J]. 中国乳业，2021（8）：9-12
[3] YANG Z M，TAO L. How to Produce high-quality silage[J]. China Dairy，2012（6）：26-32 杨茁萌，陶莲. 如何制作高品质青贮饲料[J]. 中国乳业，2012（6）：26-32
[4] ZHENG M L，NIU D Z，ZUO S S，et al. The effect of cultivar， wilting and storage period on fermentation and the clostridial community of alfalfa silage[J]. Italian Journal of Animal Science，2018，17（2）：336-346
[5] SAARISALO E，JALAVA T，SKYTTA E，et al. Effect of lactic acid bacteria inoculants， formic acid， potassium sorbate and sodium benzoate on fermentation quality and aerobic stability of wilted grass silage[J]. Agricultural and Food Science，2006，15（3）：185-199
[6] HEI M Q，CAO Z Y，WANG Z Y，et al. Effects of adding compound lactobacillus and molasses on the quality of alfalfa silage with different moisture contents[J]. Acta Agrestia Sinica，2024，32（5）：1601-1609 黑梦琴，曹正宇，王志远，等. 添加复合乳酸菌和糖蜜对不同水分含量苜蓿青贮品质的影响[J]. 草地学报，2024，32（5）：1601-1609
[7] CAI Y M，Kumai S，LIAO Z，et al. Effect of lactic acid bacteria inoculants on fermentative quality of silage[J]. Scientia Agricultura Sinica，1995（2）：73-82 蔡义民，熊井清雄，廖芷，等. 乳酸菌剂对青贮饲料发酵品质的改善效果[J]. 中国农业科学，1995（2）：73-82
[8] CARVALHO B F，SALES G F C，SCHWAN R F，et al. Criteria for lactic acid bacteria screening to enhance silage quality[J]. Journal of Applied Microbiology，2021，130（2）：341-355
[9] DONG W Z，ZHAO S，ZHANG L Q，et al. Effects of the addition of a novel compound lactic acid bacteria on alfalfa silage quality and bacterial community composition[J]. Chinese Journal of Grassland，2023，45（10）：78-86 董文卓，赵帅，张璐青，等. 一种新型复合乳酸菌剂对苜蓿青贮品质及细菌群落的影响[J]. 中国草地学报，2023，45（10）：78-86
[10] BAI J，XU D X，XIE D M，et al. Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation， aerobic stability， and microbial community of alfalfa silage[J]. Bioresource technology，2020，315：123881
[11] Li F H，Ding Z T，Chen X Z，et al. The effects of Lactobacillus plantarum with feruloyl esterase-producing ability or high antioxidant activity on the fermentation， chemical composition， and antioxidant status of alfalfa silage[J]. Animal Feed Science and Technology，2021，273：114835
[12] GAO R，HOU J J，WU Z，et al. Effects of different water and lactic acid bacteria on the quality of wrapped alfalfa silage[J]. Journal of Grassland and Forage Science，2018（S1）：74-76 高润，侯建建，吴哲，等. 水分和乳酸菌添加剂对裹包苜蓿青贮品质的影响[J]. 草学，2018（S1）：74-76
[13] LI R R，ZHENG M H，CUI X Y，et al. Screening of lactic acid bacteria and its effect on fermentation characteristics of alfalfa silage[J]. Chinese Journal of Grassland，2021，43（11）：69-75，104 李荣荣，郑猛虎，崔欣雨，等. 优良乳酸菌的筛选及对苜蓿青贮发酵品质的影响[J]. 中国草地学报，2021，43（11）：69-75，104
[14] XU Q F，YU Z，HAN J G，et al. Determining organic acid in alfalfa silage by hplc[J]. Grassland and Turf，2007（2）：63-65，67 许庆方，玉柱，韩建国，等. 高效液相色谱法测定紫花苜蓿青贮中的有机酸[J]. 草原与草坪，2007（2）：63-65，67
[15] BRODERICK G A，KANG J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science，1980，63（1）：64-75
[16] SHI H W. Determination of protein content in foods using the kjeldahl method and associated error analysis[J]. China Food Safety Magazine， 2016（11X）：1 石红伟.凯氏定氮法检测食物中蛋白质含量与误差分析[J].食品安全导刊， 2016（11X）：1
[17] ZHANG S W，ZONG Y J，FANG C Y，et al. Optimization of anthrone colorimetric method for rapid determination of soluble sugar in barley leaves[J]. Food Research and Development，2020，41（7）：196-200 张述伟，宗营杰，方春燕，等. 蒽酮比色法快速测定大麦叶片中可溶性糖含量的优化[J]. 食品研究与开发，2020，41（7）：196-200
[18] VAN SOEST P J，ROBERTSON J B，LEWIS B A. Methods for dietary fiber， neutral detergent fiber， and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science，1991，74（10）：3583-3597
[19] LI R R，HU M，WU Y Q，et al.Metagenomic structure and function analysis of clostridial community in alfalfa silage[J]. Chinese Journal of Grassland，2023，45（4）：115-120 李荣荣，胡明，吴逸群，等. 基于宏基因组的苜蓿青贮中梭菌菌群结构和功能分析[J]. 中国草地学报，2023，45（4）：115-120
[20] ZHENG M L，MAO P C，TIAN X X，et al. Effects of exogenous lactic acid bacteria and maize meal on fermentation quality and microbial community of Orychophragmus violaceus silage[J].Frontiers in Microbiology， 2023，14：1276493
[21] Guo X S，Xu D M，Li F H，et al. Current approaches on the roles of lactic acid bacteria in crop silage[J]. Microbial biotechnology，2023，16（1）：67-87
[22] AGARUSSI M C N，PEREIRA O G，SILVA V P D，et al. Fermentative profile and lactic acid bacterial dynamics in non-wilted and wilted alfalfa silage in tropical conditions[J].Molecular biology reports， 2019，46（1）：451-460
[23] ZHANG L Y. Effects of lactic acid bacteria and molasses on nutrient composition，fermentation quality and bacterial flora of alfalfa silage[D]. Tong liao：Inner Mongolia University for Nationalities，2022：14-22 张丽媛. 添加乳酸菌和糖蜜对苜蓿青贮的营养成分、发酵品质和细菌菌群的影响[D]. 通辽：内蒙古民族大学，2022：14-22
[24] LI R R，HU M，WEI R，et al. Effects of sucrose addition on fungal community diversity in high-moisture alfalfa silage[J]. Chinese Journal of Grassland，2024，46（1）：143-148 李荣荣，胡明，魏睿，等. 添加蔗糖对高水分苜蓿青贮中真菌菌群多样性的影响[J]. 中国草地学报，2024，46（1）：143-148
[25] ZHENG M L，NIU D Z，JIANG D，et al. Metagenome analyses reveal the role of Clostridium perfringens in alfalfa silage anaerobic deterioration[J]. FEMS Microbiology Letters，2020，367（8）：fnaa052
[26] JIANG H，WANG H R，BAO B H，et al. Effect of compound additives on nutritional composition， fermentation quality， and bacterial community of high-moisture alfalfa silage[J]. Fermentation，2023，9（5）：453
[27] ZHANG Y，LIU Y，MENG Q，et al. A mixture of potassium sorbate and sodium benzoate improved fermentation quality of whole‐plant corn silage by shifting bacterial communities[J]. Journal of applied microbiology，2020，128（5）：1312-1323
[28] LI P，ZHAO W J，YAN L J，et al. Inclusion of abandoned rhubarb stalk enhanced anaerobic fermentation of alfalfa on the Qinghai Tibetan Plateau[J]. Bioresource Technology，2022，347：126347
[29] WANG Y，GUO T X，CHEN D D，et al. The effects of chlorogenic acid on the quality， protein degradation， bacterial community， and their interrelationships of stylosanthes silage[J]. Acta Agrestia Sinica，2025，33（1）：317-326 王垚，郭田心，陈丹丹，等.绿原酸对柱花草青贮品质、蛋白降解和细菌群落及相互关系的影响[J]. 草地学报，2025，33（1）：317-326
[30] WANG H R，GAO F Q，XUE Y L，et al. Effects of different lactic acid bacteria additives on the quality and microbial diversity of oat silage[J]. Chinese Journal of Grassland，2024，46（11）：82-90 王昊然，高凤芹，薛艳林，等. 不同乳酸菌添加剂对燕麦青贮品质和微生物多样性的影响[J]. 中国草地学报，2024，46（11）：82-90
[31] YU X J，HU H R，CAO Y H，et al. Key to English-Chinese translation of strain names after taxonomic status change of Lactobacillus （一）[J]. Food and Fermentation Industries，2020，46（15）：318-324 于学健，胡海蓉，曹艳花，等. 乳杆菌属分类学地位变迁后菌种名称英解汉译检索表（一）[J]. 食品与发酵工业，2020，46（15）：318-324
[32] FAN X M，ZHAO S S，YANG F Y，et al. Effects of lactic acid bacterial inoculants on fermentation quality， bacterial community， and mycotoxins of alfalfa silage under vacuum or nonvacuum treatment[J]. Microorganisms，2021，9（12）：2614
[33] ZHANG X Y，ZHAO S S，WANG Y P，et al. The Effect of Lactiplantibacillus plantarum ZZU203， Cellulase-Producing Bacillus methylotrophicus， and Their Combinations on Alfalfa Silage Quality and Bacterial Community[J]. Fermentation，2023，9（3）：287
[34] MIRANDA-TELLO E，FARDEAU M L，Sepulveda J，et al. Garciella nitratireducens gen. nov.， sp. nov.， an anaerobic， thermophilic， nitrate-and thiosulfate-reducing bacterium isolated from an oilfield separator in the Gulf of Mexico[J]. International journal of systematic and evolutionary microbiology，2003，53（5）：1509-1514
[35] TIDJANI ALOU M，MILLION M，TRAORE S I，et al. Gut bacteria missing in severe acute malnutrition， can we identify potential probiotics by culturomics？[J]. Frontiers in microbiology，2017，8：899
[36] ZHENG M L，LI Q，MAO P C，et al. Identification and Correlation Analysis of Key Clostridia and LAB Species in Alfalfa Silages Prepared with Different Cultivars and Additives[J]. Agriculture，2024，14（11）：1963
[37] ZHANG J Z. Screening of GABA-Producing lentilactobacillus buchneri and its application in ensiled forages[D]. Lanzhou：Lanzhou University，2023：28-32 张家铫. 产γ-氨基丁酸布氏乳杆菌的筛选及其在饲草青贮中的应用[D]. 兰州：兰州大学，2023：28-32
[38] LU Y X，ZHAO M，CHEN L Y，et al. Effects of lactobacillus brucei and formic acid addition on fermentation quality and bacterial community of oat silage of different harvest stages in the tibetan plateau[J]. Acta Agrestia Sinica，2020，28（6）：1736-1743 陆永祥，赵嫚，陈良寅，等. 布氏乳杆菌和甲酸对青藏高原不同物候期燕麦青贮饲料发酵品质和细菌群落的影响[J]. 草地学报，2020，28（6）：1736-1743
[39] LIU Y C，LI Y Y，LU Q，et al. Effects of lactic acid bacteria additives on the quality， volatile chemicals and microbial community of leymus chinensis silage during aerobic exposure[J]. Frontiers in Microbiology，2022，13：938153
[40] ZHENG X K，FU Y B，YANG H J，et al. Isolation and identification of dominant lactic acid bacteria from brassica napus and their effects on fermentation quality and aerobic stability of silage[J]. Chinese Journal of Animal Nutrition，2024，36（8）：5411-5421 郑晓凯，付彦博，杨寒珺，等. 饲用油菜中优势乳酸菌的分离鉴定及其对青贮发酵品质和有氧稳定性的影响[J]. 动物营养学报，2024，36（8）：5411-5421
[41] ZHAO J J，JIA Y S，WANG Z J，et al. Progress study on the effects of lactobacillus buchneri on silage quality based on meta-analysis[J]. Acta Agrestia Sinica，2025，33（3）：665-677 赵佳婕，贾玉山，王志军，等. 基于Meta分析的布氏乳杆菌对青贮品质影响进展研究[J]. 草地学报，2025，33（3）：665-677
[42] OLOFSSON T C，ALSTERFJORD M，NILSON B，et al. Lactobacillus apinorum sp. nov.， Lactobacillus mellifer sp. nov.， Lactobacillus mellis sp. nov.， Lactobacillus melliventris sp. nov.， Lactobacillus kimbladii sp. nov.， Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov.， isolated from the honey stomach of the honeybee Apis mellifera[J]. International journal of systematic and evolutionary microbiology，2014，64（Pt_9）：3109-3119

Effects of Sucrose and Lactic Acid Bacteria Inoculantion on Dominant Clostridia and Lactic Acid Bacteria Communities in High-moisture Alfalfa Silage

添加蔗糖和乳酸菌剂对高水分苜蓿青贮中优势梭菌和乳酸菌菌群的影响

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