Cloning and Characterizatic Analysis of MfWRKY3 Gene in Medicago falcata

doi:10.11733/j.issn.1007-0435.2026.03.006

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (3): 796-803.DOI: 10.11733/j.issn.1007-0435.2026.03.006

Cloning and Characterizatic Analysis of MfWRKY3 Gene in Medicago falcata

ZHAO Jing-wei^1,2, JIN Xiao-wei¹, CUI Meng-lan¹, ZHANG Li-quan^1,3

1. College of Life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China;
2. Inner Mongolia Baotou No. 40 Middle School, Baotou, Inner Mongolia 014030, China;
3. Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, Hohhot, Inner Mongolia 010070, China

Received:2025-04-17 Revised:2025-07-16 Published:2026-03-23

黄花苜蓿MfWRKY3基因克隆及特性分析

赵静玮^1,2, 金宵溦¹, 崔梦兰¹, 张立全^1,3

1. 内蒙古大学生命科学学院, 内蒙古呼和浩特 010070;
2. 内蒙古包头市第四十中学, 内蒙古包头 014030;
3. 牧草与特色作物生物学教育部重点实验室, 内蒙古呼和浩特 010070

通讯作者: 张立全，E-mail：zhangliquan430@126.com
作者简介:赵静玮（1995-），女，汉族，内蒙古包头人，硕士研究生，主要从事苜蓿抗逆方面研究，E-mail：1120470879@qq.com；
基金资助:
国家自然科学基金地区项目（32460339，31460628）；内蒙古自然科学基金面上项目（2024MS03028）；内蒙古自治区高等学校科学技术研究重点项目（NJZZ22331）资助

Abstract

Abstract: To explore the role of MfWRKY3 in regulating the response of Medicago falcata to stresses， the MfWRKY3 gene was cloned from wild Medicago falcata in this study， and the characteristics of it encoded protein and the expression pattern were preliminarily identified. The results showed that the length of MfWRKY3 ORF （open reading frame） is 1575 bp， encoding a protein with 524 amino acids， predicted molecular mass of 57.36 kDa and an isoelectric point of 6.96. The MfWRKY3 had transcriptional activation activity and was localized in the nucleus， belonging to the WRKY Ⅰ sub-group transcription factor member. Meanwhile， it was found that the MfWRKY3 gene had the highest expression level in the leaves of Medicago falcate， and the its expression in the roots， stems and leaves were regulated by salt， low temperature and ABA treatment. However， the salt stress could significantly induce the expression of MfWRKY3 gene. These findings laid a foundation for further investigating the biological function and molecular mechanism of MfWRKY3 in regulating the response of Medicago falcata to abiotic stresses.

Key words: Medicago falcata, MfWRKY3, Gene cloning, Characterization analysis

摘要： 为了探究MfWRKY3在调节黄花苜蓿（Medicago falcata）响应逆境胁迫过程中发挥的作用，本研究从野生黄花苜蓿中克隆获得了MfWRKY3基因，并对其编码蛋白特性和表达模式进行了研究。结果发现，MfWRKY3基因开放阅读框为1575 bp，编码524个氨基酸的蛋白质，其分子量为57.36 kDa，理论等电点为6.96。MfWRKY3蛋白具有转录激活特性并定位在细胞核内，属于WRKY Ⅰ亚家族转录因子家族成员。MfWRKY3基因在黄花苜蓿叶中表达量最高，且在根、茎和叶中的表达均受盐和低温胁迫以及脱落酸（Abscisic acid，ABA）处理的影响。其中，盐胁迫可显著诱导MfWRKY3基因的表达。这些结果为进一步探究MfWRKY3基因调节黄花苜蓿响应逆境胁迫的生物学功能和机理奠定了基础。

关键词: 黄花苜蓿, MfWRKY3, 基因克隆, 特性分析

CLC Number:

S541.9

ZHAO Jing-wei, JIN Xiao-wei, CUI Meng-lan, ZHANG Li-quan. Cloning and Characterizatic Analysis of MfWRKY3 Gene in Medicago falcata[J]. Acta Agrestia Sinica, 2026, 34(3): 796-803.

赵静玮, 金宵溦, 崔梦兰, 张立全. 黄花苜蓿MfWRKY3基因克隆及特性分析[J]. 草地学报, 2026, 34(3): 796-803.

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Cloning and Characterizatic Analysis of MfWRKY3 Gene in Medicago falcata

黄花苜蓿MfWRKY3基因克隆及特性分析

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