Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (4): 1181-1195.DOI: 10.11733/j.issn.1007-0435.2026.04.005

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Screening of Salt-Tolerant and Growth-Promoting Strains of Cyperus esculentus Rhizosphere in Sandy Areas of South Xinjiang

ZHANG Ya-ning, MA Zheng-dong, CHEN Ying, TONG Xin, ZHANG Sheng-jie, JIA Shu-an, WANG Xu-zhe, MA Chun-hui   

  1. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China
  • Received:2025-07-30 Revised:2025-09-04 Published:2026-04-15

南疆沙区油莎豆根际耐盐促生菌株的筛选

张亚宁, 马正东, 陈莹, 佟鑫, 张盛洁, 贾舒安, 王旭哲, 马春晖   

  1. 石河子大学动物科技学院, 新疆 石河子 832000
  • 通讯作者: 王旭哲，E-mail：690953197@qq.com；马春晖，E-mail：chunhuima@126.com
  • 作者简介:张亚宁（2004-），男，汉族，河北邢台人，本科生，主要从事牧草生产与加工研究，E-mail：2357678055@qq.com；
  • 基金资助:
    兵团科技计划项目（2024DB023）；石河子大学青年拔尖计划项目（BJZK202406）；国家自然科学基金项目（32260856）；国家牧草产业技术体系项目（CARS）资助

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Abstract: To screen salt-tolerant and growth-promoting bacterial strains adapted to the extreme environment of Southern Xinjiang from the rhizosphere soil of Cyperus esculentus L. and enrich the strain resource bank for saline-alkali sandy soils in Xinjiang， this study employed the targeted screening medium culture method， shake flask culture method， and 16S rDNA identification method to isolate salt-tolerant and growth-promoting strains， determine their growth-promoting characteristics， and finally further verify the growth-promoting effects of these strains through Cyperus esculentus seed germination tests and potted seedling experiments. A total of 10 salt-tolerant and growth-promoting strains were obtained， which belonged to the genera Bacillus， Vreelandella， Exiguobacterium， and Bisbaumannia， with their salt tolerance thresholds ranging from 4% to 8% NaCl. Among these strains， strain Y-38 （Exiguobacterium acetylicum） exhibited prominent inorganic phosphorus-solubilizing ability （0.65 mg·L-1）， strain Y-35 （Exiguobacterium mexicanum） showed the highest production of indole-3-acetic acid （IAA） （64.35 mg·L-1）， and strain Y-46 （Bisbaumannia pacifica） could tolerate 8% NaCl. In the seed germination experiment， the germination rate of the Y-38 treatment group reached 82.00%， which was significantly 26.89% higher than the control group （P<0.05）； in the potted experiment， growth indicators of the Y-38 treatment group， including plant height （39.17 cm）， leaf width （5.22 mm）， and stem diameter （5.52 mm）， were all significantly higher than those of the Y-46 and Y-35 treatment groups （P<0.05）. The comprehensive ranking of the growth-promoting effects of the three strains was Y-38>Y-46>Y-35. Ultimately， the high salt tolerance and growth-promoting function of strain Y-38 （Exiguobacterium acetylicum） was confirmed， providing a bacterial strain resources for the improvement of saline-alkali soils.

Key words: Cyperus esculentus L., Rhizosphere growth-promoting bacteria, Salt-tolerant bacteria, Isolation and identification

摘要： 为从油莎豆（Cyperus esculentus L.）根际土壤中筛选适应南疆极端环境的耐盐促生菌株，丰富新疆盐碱沙土地菌株资源库，本研究采用定向筛选培养基培养法、摇瓶培养法和16S rDNA鉴定法，对耐盐促生菌进行筛选，并测定其促生特性。最终通过油莎豆种子萌发和盆栽幼苗试验，进一步验证其促生效果。本研究共获得10株耐盐促生菌，分属于芽孢杆菌属（Bacillus）、弗里兰氏菌属（Vreelandella）、微小杆菌属（Exiguobacterium）和比斯鲍曼氏菌属（Bisbaumannia），耐NaCl阈值范围为4%~8%。其中菌株Y-38（Exiguobacterium acetylicum）溶无机磷能力突出（0.65 mg·L-1），Y-35（Exiguobacterium mexicanum）产吲哚-3-乙酸（Indole-3-acetic acid，IAA）量最高（64.35 mg·L-1），Y-46（Bisbaumannia pacifica）耐受8%NaCl。种子萌发试验中，Y-38处理组发芽率达82.00%，较对照显著提高26.89%（P<0.05）；盆栽试验中Y-38处理组的株高（39.17cm）、叶宽（5.22mm）、茎粗（5.52mm）等指标均显著高于Y-46和Y-35处理组（P<0.05）。三株菌的促生效果通过综合排序为：Y-38>Y-46>Y-35，最终确定了Y-38（乙酰微小杆菌，Exiguobacterium acetylicum）的高耐盐性与促生功能，为盐碱土壤改良提供了菌种资源。

关键词: 油莎豆, 植物根际促生菌, 耐盐菌, 分离鉴定

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