Isolation， Identification， and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28

doi:10.11733/j.issn.1007-0435.2026.04.006

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (4): 1196-1204.DOI: 10.11733/j.issn.1007-0435.2026.04.006

Isolation， Identification， and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28

PENG Chao^1,2, YANG Lan^1,2, CUI Wen-jing^1,2, MA Yong-qiang^1,2,3, CHENG Liang^1,2,3, ZHU Hai-xia^1,2,3

1. Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai Province 810016, China;
2. Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Xining, Qinghai Province 810016, China;
3. Scientific Observation and Experiment Station of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai Province 810016, China

Received:2025-06-03 Revised:2025-07-16 Published:2026-04-15

杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价

彭超^1,2, 杨兰^1,2, 崔文静^1,2, 马永强^1,2,3, 程亮^1,2,3, 朱海霞^1,2,3

1. 青海大学农林科学院, 青海西宁 810016;
2. 青海省农业有害生物综合治理重点实验室, 青海西宁 810016;
3. 农业农村部西宁作物有害生物科学观测实验站, 青海西宁 810016

通讯作者: 朱海霞，E-mail：zhuhaixia0101@163.com；马永强，E-mail：mayongqiang_163@163.com
作者简介:彭超（2001-），男，汉族，江西吉安人，硕士研究生，主要从事生物防治研究，E-mail：pengchao0201@163.com；
基金资助:
国家自然科学基金项目（32460683）；青海省科技厅基础研究项目（2024-ZJ-928）资助

Abstract

Abstract: To screen biocontrol strains with high herbicidal potential， the pathogenic fungus JZ-28 was isolated from naturally infected leaves of Viola philippica in Qinghai Province. Through morphological characterization and multi-gene （ITS， EF-1α， LSU） phylogenetic analysis， the isolate was identified as Clonostachys rosea. Pathogenicity assays demonstrated potent herbicidal activity of strain JZ-28， inducing chlorosis in detached leaves of four dominant broadleaf weed species from the Qinghai-Xizang Plateau（Malva verticillata， Elsholtzia densa， Senecio vulgaris， and Chenopodium album）within 7 days post-inoculation （dpi）. Disease incidence rates in whole-plant assays reached 83%， 95%， 100%， and 70%， respectively. Safety evaluation on six major crops and three cool-season vegetables in Qinghai revealed that JZ-28 was safe for four crops： Zea mays， Vicia faba， Pisum sativum， Brassica napus， and three vegetables： Cucumis sativus， Solanum lycopersicum， and Solanum melongena. Lightly Susceptible was observed in highland barley Hordeum vulgare and Triticum aestivum. These findings indicated JZ-28’s potential as a broad-spectrum bioherbicide against broadleaf weeds in multiple cropping systems. Culture optimization identified sucrose and soybean meal as optimal carbon and nitrogen sources for enhancing mycelial growth and sporulation. This study provided the first evidence of Clonostachys rosea’s bioherbicide potential in alpine ecosystems， addressing a critical research gap in microbial herbicide development and offering sustainable weed management solutions for fragile environments.

Key words: Weed control, Clonostachys rosea, Microbial herbicides

摘要： 为筛选具有高效除草潜力的生防菌株，本研究从青海省自然感病紫花地丁（Viola philippica）叶片中分离出致病菌JZ-28。通过形态学及多基因（ITS/EF-1α/LSU）联合鉴定，确定为粉红螺旋聚孢霉（Clonostachys rosea）。致病性试验表明其具有强效除草活性，JZ-28在7 d可使青藏高原4种优势阔叶类杂草冬葵（Malva verticillata）、密花香薷（Elsholtzia densa）、欧洲千里光（Senecio vulgaris）和藜（Chenopodium album）的离体叶片黄化发病，活体植株试验发病率分别为83%，95%，100%，70%。评价JZ-28对青海省6种主栽作物和3种冷凉蔬菜的安全性，表明该菌株对玉米（Zea mays）、蚕豆（Vicia faba）、豌豆（Pisum sativum）、油菜（Brassica napus）4种作物和黄瓜（Cucumis sativus）、番茄（Solanum lycopersicum）、茄子（Solanum melongena）3种冷凉蔬菜表现为安全，对青稞（Hordeum vulgare）、小麦（Triticum aestivum）表现为轻微感病。培养条件优化试验表明，蔗糖与大豆粉分别为最佳碳、氮源，可促进菌丝生长与孢子产量提高。本研究首次揭示了粉红螺旋聚孢霉作为高寒地区生物除草剂的潜力，为脆弱生态系统的杂草可持续管理提供了新的途径。

关键词: 杂草防控, 粉红螺旋聚孢霉, 微生物除草剂

CLC Number:

S451

PENG Chao, YANG Lan, CUI Wen-jing, MA Yong-qiang, CHENG Liang, ZHU Hai-xia. Isolation， Identification， and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28[J]. Acta Agrestia Sinica, 2026, 34(4): 1196-1204.

彭超, 杨兰, 崔文静, 马永强, 程亮, 朱海霞. 杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价[J]. 草地学报, 2026, 34(4): 1196-1204.

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Isolation， Identification， and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28

杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价

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