Cloning， Expression， and Functional Analysis of EARLY RESPONSIVE TO DEHYDRATION 15 gene from Medicago sativa L.

doi:10.11733/j.issn.1007-0435.2026.08.009

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (8): 2847-2856.DOI: 10.11733/j.issn.1007-0435.2026.08.009

Cloning， Expression， and Functional Analysis of EARLY RESPONSIVE TO DEHYDRATION 15 gene from Medicago sativa L.

ZHANG Wei-yan¹, LI Yun¹, LIU Yan-kun¹, WANG Hao¹, LIU Qing-song²

1. Cangzhou Technical College, Cangzhou, Hebei Province 061000, China;
2. Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, Hebei Province 061000, China

Received:2025-09-15 Revised:2025-12-16 Published:2026-08-04

紫花苜蓿EARLY RESPONSIVE TO DEHYDRATION 15基因克隆、表达及功能分析

张伟燕¹, 李云¹, 刘艳昆¹, 王皓¹, 刘青松²

1. 沧州职业技术学院, 河北沧州 061000;
2. 沧州市农林科学院, 河北沧州 061000

通讯作者: 王皓,E-mail:1315337220@qq.com
作者简介:张伟燕(1979-),女,汉族,河北沧州人,硕士,副教授,主要从事园林专业植物育种研究,E-mail:601328274@qq.com
基金资助:
田菁改良盐碱地技术研究（CCPTZX2024GJ02-1-4）资助

Abstract

Abstract: ERD15 （EARLY RESPONSIVE TO DEHYDRATION 15） is an important regulatory factor involving in plant stress responses. In this study， the MsERD15 gene from alfalfa （Medicago sativa L.） was cloned， and its potential role in stress tolerance was investigated through bioinformatics， expression profiling， and functional analyses. The MsERD15 gene was cloned by RT-PCR， and the physicochemical properties， conserved domains， and phylogenetic relationships of the encoded protein were analyzed. The expression patterns of MsERD15 under drought， high salinity， and abscisic acid （ABA） treatments were examined using qRT-PCR. A MsERD15-YFP fusion construct was generated for subcellular localization analysis， and heterologous expression in yeast was performed to evaluate its stress tolerance function. The results showed that the coding sequence of MsERD15 was 402 bp in length， encoding a protein of 134 amino acids. The gene was significantly induced by drought， high salinity， and ABA treatments. Subcellular localization indicated that the protein was localized in the cytoplasm. Expression of MsERD15 in yeast significantly enhanced tolerance to high salinity and polyethylene glycol （PEG） stress. These results indicated that MsERD15 participated in stress responses and night serve as a candidate gene for improving stress tolerance in alfalfa through molecular breeding.

Key words: Alfalfa, MsERD15, Expression analysis, Cytoplasm localization, Yeast, Abiotic stress

摘要： ERD15（EARLY RESPONSIVE TO DEHYDRATION 15）是植物逆境响应的重要调控因子。本研究克隆紫花苜蓿（Medicago sativa L.）MsERD15基因，并通过生物信息学、表达模式及功能分析探讨其抗逆作用。利用RT-PCR克隆MsERD15基因并分析蛋白理化性质、结构域及系统进化关系；qRT-PCR检测干旱、高盐及脱落酸（Abscisic acid， ABA）处理下的表达模式；构建MsERD15-YFP融合载体进行亚细胞定位；通过酵母异源表达验证抗逆功能。结果表明：MsERD15编码区全长402 bp，编码134个氨基酸；该基因受干旱、高盐及ABA显著诱导；蛋白定位于细胞质。酵母表达MsERD15可显著提高对高盐和聚乙二醇（Polyethylene glycol，PEG）胁迫的耐受性。MsERD15参与逆境响应，可作为紫花苜蓿抗逆分子育种的候选基因。

关键词: 紫花苜蓿, MsERD15, 表达分析, 细胞质定位, 酵母, 非生物胁迫

CLC Number:

S541.9

ZHANG Wei-yan, LI Yun, LIU Yan-kun, WANG Hao, LIU Qing-song. Cloning， Expression， and Functional Analysis of EARLY RESPONSIVE TO DEHYDRATION 15 gene from Medicago sativa L.[J]. Acta Agrestia Sinica, 2026, 34(8): 2847-2856.

张伟燕, 李云, 刘艳昆, 王皓, 刘青松. 紫花苜蓿EARLY RESPONSIVE TO DEHYDRATION 15基因克隆、表达及功能分析[J]. 草地学报, 2026, 34(8): 2847-2856.

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Cloning， Expression， and Functional Analysis of EARLY RESPONSIVE TO DEHYDRATION 15 gene from Medicago sativa L.

紫花苜蓿EARLY RESPONSIVE TO DEHYDRATION 15基因克隆、表达及功能分析

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