Acta Agrestia Sinica

›› 1999, Vol. 7 ›› Issue (4): 287-292.DOI: 10.11733/j.issn.1007-0435.1999.04.005

Previous Articles     Next Articles

RAPD Analysis on the Genomic DNA of Several Triticeae Grasses and Their Hybrids

Yu Zhuo1,2, Yun Jinfeng1,2, Ma Youzhi2, Xin Zhiyong2   

  1. 1. Inner Mongolia Agricultural University, Hohhot 010019;
    2. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Online:1999-11-15 Published:1999-11-15

几种小麦族禾草及其杂交后代基因组DNA的RAPD研究

于卓1,2, 云锦凤1,2, 马有志2, 辛志勇2   

  1. 1. 内蒙古农业大学, 呼和浩特 010019;
    2. 中国农业科学院作物育种栽培研究所, 北京 100081
  • 基金资助:
    国家自然科学基金;农业部作物遗传育种重点实验室开发性课题基金

PDF

Abstract: The Genomic DNA differences among Agropyron mongolicum ? Agropyrom cristatum cv.Fairway F1 hybrid,their backcross 1 (F1 hybrid ? A.mongolicum)and Elymus canadensis ? Hordeum brevisubulatum F1 hybrid were analyzed by using RAPD.The appropriate primers which can be used to determine the genetic differences among parents and their hybrids were selected.It was tested and verified thatthe genetic differences in DNA between E.canadensis and H.brevisubulatum were greater than that between A.mongolicum and A.cristatum cv.Fairway.The RAPD profiles of F1 hybrids tended to their motherobviously.RAPD can be used to identify hybrids and to study genetic breeding of grasses.

Key words: Trticeae grasses, Hybrid, Genomic DNA, RAPD, Appropriate primers

摘要： 应用随机扩增多态性DNA(RAPD)技术分析蒙古冰草、航道冰草及其种间杂种F1、BC1代和加拿大披碱草、野大麦及其属间杂种F1代的基因组DNA差异性。筛选出能检测各条本及杂交后代间遗传差异的适宜引物;从DNA分子水平验证出亲本加拿大披碱草和野大麦两属间种比亲本蒙古冰草、航道冰草二种间的亲缘关系远;杂种F1代的RAPD图谱有明显偏母本的倾向;随机扩增多态性DNA技术可用于牧草远缘杂种鉴定及遗传育种研究。

关键词: 小麦族禾草, 杂交种, 基因组DNA, 随机扩增多态性DNA, 适宜引物