Abstract: A systemic study of plant regeneration of perennial ryegrass has been conducted.The result showed the modified MS medium (MSM) that composed of MS salt +9.9 mg/L vitamin B₁+9.5 mg/L vitamin B₆+4.5 mg/L nicotinic acid+1 mg/L hydrolyze casine+30 g/L sucrose+8 g/L agar as basic medium was suitable for inducing callus formation from ryegrass seeds when 5 mg/L 2,4-D and 0.05 mg/L KT added.The subculture medium containing 2 mg/L 2,4-D and 0.1 mg/L KT.The medium for callus differentiation into plantlets containing 1 mg/L 2,4-D and 1 mg/L KT.The medium for regenerated plantlets rooting was basic MS medium free of hormone.The whole period of ryegrass plant regeneration takes about 12 weeks.The differentiation ratio of calli reached 70%.

Key words: Biotechnology, Perennial ryegrass, Tissue culture, Plant regeneration

摘要： 对多年生黑麦草种子为外植体的植株再生过程进行系统研究,结果表明,在改良的MS培养基(MSM),以MS无机盐+9.9mg/L维生素B₁+9.5mg/L维生素B₆+4.5mg/L尼克酸+1mg/LCH+30g/L蔗糖+琼脂8g/L为基本成分,附加5mg/L2,4-D和0.05mg/LKT时,适合种子的愈伤组织诱导;继代培养基附加2mg/L2,4-D和0.1mg/LKT;分化培养基附加1mg/L2,4-D和1mg/LKT;生根培养基为无激素的MS培养基。完成植株再生约需12周,愈伤组织分化率为70%。

关键词: 生物技术, 多年生黑麦草, 组织培养, 再生