Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)

doi:10.11733/j.issn.1007-0435.2004.03.007

›› 2004, Vol. 12 ›› Issue (3): 199-203.DOI: 10.11733/j.issn.1007-0435.2004.03.007

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Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)

CHENG Lin-mei¹, CAO Qiu-fen¹, GAO Hong-wen², HUANG Jing¹, MENG Yu-pin¹

1. Shanxi Agri-Biotechnolgy Research Center, Taiyuan, ShanxiProvince, 030031, China;
2. Research and Development Center for Grassland Science, ChinaAcademy of Agriculture Sciences, Beijing, 10081, China

Received:2003-09-10 Revised:2003-12-25 Online:2004-08-15 Published:2004-08-15

菊苣再生体系建立及转AFL2基因的研究

程林梅¹, 曹秋芬¹, 高洪文², 黄静¹, 孟玉平¹

1. 山西省农业生物技术研究中心, 太原, 030031;
2. 中国农业科学院草业研究中心, 北京, 100081

作者简介:程林梅(1954- ),女,山西人,副研究员,主要从事作物抗旱生理、生物技术方面的研究
基金资助:
国家"863"计划(2001AA241165);山西省留学回国人员资助项目(20011575)

Abstract

Abstract: A research was conducted using leaves of Puna chicory as explants to establish a highly efficient Puna chicory regeneration system and its stable agrobacterium mediated transformation system. The result shows that the chicory regenerating frequency was increased from 48% to 100%, while the number of regenerating single leaf buds appearing as many as 12. After three days of culturing the chicory leaf seedlings with agrobacterium strain FIG 121 Hm, and two weeks in regenerate medium containing 20 mg/L kanmycin and 600 mg/L cephalosorine, the chicory leaf seedlings regenerated into transgenic buds, with the regenerating rate reaching 8.3%. The buds could develop in a medium of 30 mg/L kanamycin and reproduce successively, which is tentatively determined to be transgenic buds.

Key words: Biotechnology, Cichorium intybus L. cv.Puna, Plant regeneration, AFL2 gene, Genetic transformation

摘要： 以菊苣叶片为外植体,研究建立高效再生体系和农杆菌介导的稳定转化体系。结果表明:再生频率从48%提高至100%;单叶盘再生芽数达12个;试管苗叶片与农杆菌菌株PIG121Hm共培养3d,在附加卡那霉素20mg/L和头孢霉素600mg/L再生培养基上选择培养2周后,叶片再生出转化芽,绿芽率达8.6%,转化芽在附加30mg/L卡那霉素培养基中长大并继代增殖,初步鉴定是转化芽。

关键词: 生物技术, 菊苣, 再生, AFL2基因, 遗传转化

CLC Number:

Q943

CHENG Lin-mei, CAO Qiu-fen, GAO Hong-wen, HUANG Jing, MENG Yu-pin. Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)[J]. , 2004, 12(3): 199-203.

程林梅, 曹秋芬, 高洪文, 黄静, 孟玉平. 菊苣再生体系建立及转AFL2基因的研究[J]. , 2004, 12(3): 199-203.

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References

[1] 高洪文.高产优质饲用植物-菊苣[J].山西农业科学(增刊),1993,1～3
[2] 高洪文,马明荣,杨清娥.菊苣开花及种子形成规律[J].山西农业科学(增刊),1993,17～20
[3] 刘建宁,贺东昌.北方干旱地区牧草栽培与利用[M].金盾出版社,145～148
[4] 王绍明,张霞,刘彤.菊苣花瓣的组织培养[J].植物生理学通讯,2001,37(3):230
[5] Nenz E,Varottos,lucchin M,Parrinip.An efficient and rapid procedure for plantlet regeneration from chicory mesophyll pratoplasts[J].Plant Cell Tissue and Organ Culture,2000,62(1):85～88
[6] Varotts,lucchin M,Parrinip.Immature embryos culture in Italian red chicory [J].Plant Cell Tissue and Organ Culture,2000,62(1):75～79
[7] Frulleux F,Weyens G,Jacobs m.Agrobacterium tumefaciens mediated transformation of shoot buds of chicory[J].Plant Cell Tissue and Organ Culture,1997,50(2):107～112
[8] 李颖章,韩碧文.薄层培养的菊苣不定根分化中内源IAA和细胞分裂的动态变化[J].植物生物学通讯,1995,31(2):97～99
[9] Masato Wata,Qiu-fen Cao,Nobuhiro Kotoda,et al.Apple has two orthologues of FLORICAULA/LEAFY involved in flowering [J].Plant Molecular Biology,2002,49(6):567～577
[10] Horsch R B,Fry J E,Hoffmann N L,Eichholtz D,Rogers S G,Fraley R T.A simple and general method for transforming genes into plant[J].Science,1985,227:1229～ 1231
[11] 张志宏,吴禄平.草莓主栽品种TudIa遗传转化体系的建立[J].农业生物技术学报,1998,6(2):200～204
[12] Bousquet T,Simon L,Lalonde M.DNA amplification from vegetative and sexal tissues of trees using polymerase chain reaction[J].Can.J.For.Res,1990,20:254～257
[13] Murray G C,Thompson W F.Rapid isolation of high molecular weight DNA[J].Nucl.Acid Res,1980,8:4321 ～ 4325
[14] 周冀明,卫志明,许智宏,等.根癌农杆菌介导转化诸葛菜子叶获得转基因植株[J].生物工程学报,1996,12(1):34～39
[15] Joersbo M,Okkels F T.Anovel principle for selection of transgenic plant cells.Positive selection,plant ceu rep,1996,16:219～221
[16] Nehra N S,Chibbar R N,Kartha K K,Datla R S S,Crosby W L,Stushnoff C.Genetic transformation of strawberry by agrobacterium tumefaciens using a leaf disk regeneration system [J].Plant Cell Reports,1990,9:293～ 298
[17] 蓝海燕,陈正华.农杆菌介导的芸苔属植物遗传转化技术的研究进展[J].生物技术通报,1999,(3),15～18
[18] 叶兴国,Shirley Sato,徐惠君,杜丽璞,等.小麦农杆菌介导转基因植株的稳定获得和检测[J].中国农业科学,2001,34(5):469～474
[19] 黄璐,卫志明.农杆菌介导的遗传转化[J].实验生物学报,1999,5(32):381～387
[20] 李洪清,李美茹.影响农杆菌介导植物基因转化的因素问题[J].植物生理学通讯,1994,35:145～151

Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)

菊苣再生体系建立及转AFL2基因的研究

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