RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province

doi:10.11733/j.issn.1007-0435.2008.02.006

›› 2008, Vol. 16 ›› Issue (2): 129-134.DOI: 10.11733/j.issn.1007-0435.2008.02.006

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RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province

YANG Xiao-li, CHEN Li, BAN Ting, HAN Peng, WANG Xiao-juan

College of Pastoral Agriculture Science and Technology, Lanzhou University;Gansu GrasslandEcological Research Institute, Lanzhou, Gansu Province 730020, China

Received:2007-05-28 Revised:2007-09-24 Online:2008-04-15 Published:2008-04-15

甘肃省苜蓿种质资源遗传多样性RAPD分析

杨晓莉, 陈丽, 班霆, 韩鹏, 王晓娟

兰州大学草地农业科技学院, 甘肃省草原生态研究所, 甘肃, 兰州, 730020

通讯作者: 王晓娟,E-mail:xiaojuanwang@lzu.edu.cn
作者简介:杨晓莉(1980- ),女,甘肃武威人,硕士研究生,研究方向为牧草遗传育种,E-mail:yangx105@lzu.cn
基金资助:
国家重点研究发展计划项目(2007CB108904);甘肃省自然科学基金项目(3ZS051-A25-066);兰州大学引进人才专项基金项目(582402)

Abstract

Abstract: Alfalfa(Medicago sativa L.) is a forage legume of world-wide importance and the genetic relationship and distance among cultivars are of great interest for breeding programs.In this paper,random amplified polymorphic DNA(RAPD) was used to analyze the genetic diversity of 11 alfalfa cultivars.A total of 132 discernible loci were obtained for all populations using 10 primers,and 81.1% of these loci were polymorphic,which indicated a high genetic diversity in the cultivars from Gansu Province.According to the Roger’s distance,the smallest MRD(0.1406) occurred between cultivars ‘Tianshui’ and ‘Longzhong’,while the largest(0.4610) was detected between cultivars ‘Tianshui’ and ‘Dingxi’.The result of AMOVA analysis showed that the majority(61.25%) of genetic variation was within populations and 38.75% among populations.

Key words: Medicago spp., Germplasm, Genetic diversity, RAPD

摘要： 苜蓿(Medicago sativa L.)是世界上重要的豆科牧草,了解栽培品种的遗传关系和遗传距离对于育种工作具有重要意义。本研究利用RAPD标记技术对甘肃省11个苜蓿栽培品种(群)进行遗传多样性分析,以期为进一步开展苜蓿新品种选育奠定基础。结果表明:10个随机引物共检测到132条谱带,其中107条为多态性带,多态性条带比率(PPB)高达81.1%;天水苜蓿和定西苜蓿之间的Roger’s遗传距离较远(0.4610),而与陇中苜蓿之间的遗传距离较近(0.1406);进一步的AMOVA分析显示苜蓿种群大部分的遗传变异发生在种群内(61.25%),有38.75%的遗传变异发生在种群间,种群分化不明显。

关键词: 苜蓿, 种质资源, 遗传多样性, RAPD

CLC Number:

S812
Q943

YANG Xiao-li, CHEN Li, BAN Ting, HAN Peng, WANG Xiao-juan. RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province[J]. , 2008, 16(2): 129-134.

杨晓莉, 陈丽, 班霆, 韩鹏, 王晓娟. 甘肃省苜蓿种质资源遗传多样性RAPD分析[J]. , 2008, 16(2): 129-134.

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References

[1] Armstrong J M.Cytological studies in alfalfa polyploids[J].Canadian Journal of Botany,1954,32:531-542
[2] Lesins K A,Lesins I.Genus Medicago (leguminosae):a taxogenetic study[M].Netherlands:The Hague,Junk W Publisher,1979
[3] Kidwell K K,Austin D F.RFLP evaluation of nine Medicago accessions representing the original germplasm sources for North American alfalfa cultivars[J].Crop Science,1994,34:230-236
[4] 姜华,毕玉芬,何承刚,等.紫花苜蓿花部特征遗传多样性分析[J].遗传,2005,27(3):391-394
[5] 李拥军,苏加楷.中国苜蓿地方品种种子储藏蛋白标记-遗传多样性的研究[J].草地学报,1998,6(3):197-205
[6] 毛培胜,张涛,杨青川.紫花苜蓿品种鉴定的RAPD分子标记技术研究[J].草地学报,2007,15(2):124-128
[7] Mengoni A,Gori A,Bazzicalupo M.Use of RAPD and micro-satellite (SSR) variation to assess genetic relationships among populations of tetraploid alfalfa (Medicago sativa)[J].Plant Breeding,2000,119:311-317
[8] Yu K F,Pauls K P.Rapid estimation of genetic relatedness a-mong heterogeneous populations of alfalfa by random amplifi-cation of bulked genomic DNA samples[J].Theoretical Ap-plied Genetics,1993,86:788-794
[9] 魏臻武.利用SSR、ISSR和RAPD技术构建苜蓿基因组DNA指纹图谱[J].草业学报,2004,13(3):62-67
[10] 杨青川,韩建国.RAPD技术在苜蓿耐盐遗传育种中的应用[J].草地学报,2003,11(2):27-32
[11] 康俊梅,杨青川,樊奋成.RAPD技术分析不同抗旱性苜蓿品种DNA的多态性[J].生物技术,2005,15(6):37-40
[12] Barcaecia G,Tosti N,Falistocco E,et al.Morphological and molecular analyses of controlled progenies from meiotic mu-tants of alfalfa producing unreduced gametes[J].Theoretical and Applied Genetics,1995,91:1008-1015
[13] Kiss G B,Csanadi G,Kalman K,et al.Construction of a bas-ic genetic map for alfalfa using RFLP,RAPD isozyme and morphological markers[J].Molecular and general genetics,1993,238..129-137
[14] 李拥军,苏加楷.苜蓿地方品种遗传多样性的研究-RAPD标记[J].草地学报,1998,2(6):105-113
[15] Saghai-Maroof M A,Soliman K M,Jorgensen R A,et al.Ri-bosomal DNA spacer length polymorphism in barley[J].Pro-ceedings of the National Academy Science of the United States of American,1984,81:8104-8108
[16] 侯大斌,任正隆,舒光明.附子野生资源群体遗传多样性的RAPD分析[J].生态学报,2006,26(6):1833-1841
[17] Ercan A G,Taskin M,Turgut K.Analysis of genetic diversi-ty in Turkish sesame (Sesamum indicum L.) populations u-sing RAPD markers[J].Genetic Resources and Crop Evolu-tion,2004,5,599-607
[18] Mather K.Segregation and linkage in autotetraploids[J].Genet,1936,32,287-314
[19] 毕玉芬,车伟光,李季蓉.利用RAPD技术研究弱秋眠性紫花苜蓿遗传多样性[J].作物学报,2005,31(5):647-652
[20] Reisch C,Poschlod P,Wingender R.Genetic differentiation a-mong populations of Sesleria albicans Kit.ex Schuhea (poa-ceas) from ecologically different habitats in central Europe[J].Heredity,2003,91:519-527
[21] Musial J M,Basford K E,Iiwrin J A G.Analysis of genetic diversity within Australian lucerne cultivars and implications for future genetic improvement Australian[J].Journal of ag-ricultural research,2002,6:629-636
[22] 王中仁.植物等位酶分析[M].北京:科学出版社,1996.120-163
[23] Hamrick J L,Godt M J.Allozyme diversity in plant species[A].In,Brown A H D,Clegg M T,Kahler A L.eds.Plant Population genetics,breeding and genetic resources[C].Sun-derland,Sinauer,Associates Inc.,Sunderland Publishers,1989.43-63
[24] Weiss M R.Floral color changes as cues for pollinators[J].Nature,1991,354:227-229
[25] Amos W,Balmford A.When does conservation genetics mat-ter[J].Heredity,2001,87:257-265

RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province

甘肃省苜蓿种质资源遗传多样性RAPD分析

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