Changes in Defendant Enzyme Activity and Expression of PAL Gene of Stylosanthes after the Vaccination with Anthracnose

doi:10.11733/j.issn.1007-0435.2008.06.008

›› 2008, Vol. 16 ›› Issue (6): 585-589.DOI: 10.11733/j.issn.1007-0435.2008.06.008

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Changes in Defendant Enzyme Activity and Expression of PAL Gene of Stylosanthes after the Vaccination with Anthracnose

ZHAO Ying^1,2, FU Hai-tian³, TIAN Wei-min¹, JIANG Chang-shun¹

1. Key Laboratory for Tropical Crop Physiology, Ministry of Agriculture and Chinese Academy of Tropical Agricultural Science, Danzhou, Hainan Province 571737, China;
2. College of Agronomy, Hainan University, Danzhou, Hainan Province 571737, China;
3. Guangxi Subtropical Crops Research Institute, Nanning, Guangxi Province 530001, China

Received:2008-01-15 Revised:2008-03-04 Online:2008-12-15 Published:2008-12-15

接种后柱花草防御酶活性变化及PAL基因表达分析

赵英^1,2, 付海天³, 田维敏¹, 蒋昌顺¹

1. 中国热带农业科学院农业部热带作物栽培生理学重点开放实验室, 海南, 儋州, 571737;
2. 海南大学农学院, 海南, 儋州, 571737;
3. 广西亚热带作物研究所, 广西, 南宁, 530001

通讯作者: 蒋昌顺,E-mail:changshunj@yahoo.com.cn
作者简介:赵英(1980- ),女,河南信阳人,硕士研究生,研究方向为热带牧草分子生物学,E-mail:zhaoying-222@163.com.
基金资助:
国家自然科学基金(No.30460087);华南热带农业大学科技基金(No.Rnd0407);热带作物栽培生理学重点实验室开放课题基金(KLOF0510)资助

Abstract

Abstract: Leaves of anthracnose resistant cultivar Stylosanthes guianensis(Aubl.) Sw.cv.Mineirao and anthracnose sensitive cultivar S.guianensis cv.CIAT2312 were tested for activities of phenylalanine ammonialyase(PAL),polyphenol oxidase(PPO),and superoxide dismutase(SOD) after inoculation of Collectotrichum gloeosporioides(Penz.) Sacc.The results showed that the activities of the three enzymes were similar in Mineriao and CIAT2312.After inoculation,the activities of the three enzymes increased at first and declined afterward,the increment of Mineriao was more than CIAT2312.It was concluded that the variation of PAL,PPO,SOD activities in the inoculated leaves were closely correlated with the Stylosanthes cultivar resistance against anthracnose.PAL gene was cloned from Mineirao and its expression level was higher than CIAT2312 after inoculation by the test of semi-quantitative RT-PCR.We speculated that PAL gene had function in the anthracnose resistance of Stylosanthes.

Key words: Stylosanthes, Phenylalanine ammonialyase, Polyphenol oxidase, Superoxide dismutase, PAL gene

摘要： 在抗病Mineriao和感病CIAT2312柱花草(Stylosanthes spp.)叶片接种炭疽病病原菌后,测定苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)的活性。结果表明:接种后两种柱花草种质3种酶活性均先升后降,其中抗病株高于感病株,酶活性与柱花草对炭疽病的抗性密切相关;从Mineirao柱花草中克隆出PAL基因,半定量RT-PCR分析表明,其表达量高于CIAT2312柱花草,初步推测柱花草PAL基因对柱花草具有抗炭疽病作用。

关键词: 柱花草, 苯丙氨酸解氨酶, 多酚氧化酶, 超氧化物歧化酶, PAL基因

CLC Number:

Q946.5

ZHAO Ying, FU Hai-tian, TIAN Wei-min, JIANG Chang-shun. Changes in Defendant Enzyme Activity and Expression of PAL Gene of Stylosanthes after the Vaccination with Anthracnose[J]. , 2008, 16(6): 585-589.

赵英, 付海天, 田维敏, 蒋昌顺. 接种后柱花草防御酶活性变化及PAL基因表达分析[J]. , 2008, 16(6): 585-589.

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Changes in Defendant Enzyme Activity and Expression of PAL Gene of Stylosanthes after the Vaccination with Anthracnose

接种后柱花草防御酶活性变化及PAL基因表达分析

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