Agronomic Traits and Genetic Diversity Analysis of M3 Generation of Stylosanthes spp. Irradiated by 60Coγ

doi:10.11733/j.issn.1007-0435.2021.09.016

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (9): 1992-2000.DOI: 10.11733/j.issn.1007-0435.2021.09.016

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Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ

LUO Ming-xin¹, LIU Feng-min², CHEN Ji-yan^1,3, CUI Jun-tao^3,4, ZHANG Wei-li^1,3

1. College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510225, China;
2. Management Center of Teaching and Research Base, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510225, China;
3. Deqing Zhongkai Agricultural Tech. Innovation Research Co. Ltd., Zhaoqing, Guangdong Province 526642, China;
4. College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510225, China

Received:2021-03-03 Revised:2021-04-12 Online:2021-09-15 Published:2021-10-14

⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析

罗铭欣¹, 刘凤民², 陈纪言^1,3, 崔均涛^3,4, 张伟丽^1,3

1. 仲恺农业工程学院农业与生物学院, 广东广州 510225;
2. 仲恺农业工程学院教学科研基地管理中心, 广东广州 510225;
3. 德庆仲恺农业产业科技创新研究有限公司, 广东肇庆 526642;
4. 仲恺农业工程学院园艺园林学院, 广东广州 510225

通讯作者: 张伟丽,E-mail:zhangweili7218@163.com
作者简介:罗铭欣(1996-),女,广东德庆人,硕士研究生,主要从事作物遗传育种研究,E-mail:734486374@qq.com
基金资助:
广东省科技计划项目（2017A020208073）；广东省现代农业产业技术体系创新团队（2020KJ114）资助

Abstract

Abstract: To explore the effects of radiation on the agronomic traits of Stylosanthes spp. M₃ generation and molecular marker-assisted mutation breeding technology, plant height, crown diameter, number of branches, first branch length, disease grade, flowering stage and dry weight per plant were measured and genetic diversity analysis of conserved DNA-derived polymorphism and sequence related amplified polymorphism was made by using 17 samples of M₃ generation Stylosanthes spp. seeds that were mutagenized by ⁶⁰Coγ-ray radiation and selected with excellent traits after 2 consecutive generations of single plant selection from M₂ generations. The genetic similarity coefficients of 17 Stylosanthes spp. were calculated using NTSYS-pc 2.1 software, as a result, 92 and 177 bands were amplified by 17 CDDP primers and 26 pairs of SRAP primers respectively, in which the number of polymorphic bands were 66 and 111 respectively, the polymorphism rates were 71.7% and 62.7%, the specific bands were 13 and 29, and the specificity rates were 14.1% and 16.4% respectively. The genetic similarity coefficient (GS) between germplasms ranged from 0.54~0.90 and 0.60~0.94, and the average genetic similarity coefficient were 0.76 and 0.79 respectively. The cluster map of 17 Stylosanthes spp. showed the 17 Stylosanthes spp. could be divided into 7 groups and the clustering results were basically consistent with the statistical results of agronomic traits when the genetic similarity coefficient was 0.82 according to the unweighted paired arithmetic average method and made based on the combination of CDDP and SRAP markers. This study provides a certain reference basis for the breeding of Stylosanthes spp. by radiation mutation in the future.

Key words: Stylosanthes spp., ⁶⁰Coγ-ray, Agronomic characters, CDDP, SRAP, Genetic diversity

摘要： 为了探究辐射对柱花草（Stylosanthes spp.） M₃代农艺性状的影响及分子标记辅助诱变育种技术，利用经⁶⁰Coγ射线辐射诱变后的柱花草种子，种植后经过连续2代的单株选择筛选到17份性状优良的M₂代柱花草种子，种植成为M₃代后，对17份柱花草测定其株高、冠径、分枝数、第一分支长、病害级数、开花期、单株干重，并进行DNA保守序列多态性（Conserved DNA-derived polymorphism，CDDP）和相关序列扩增多态性（Sequence related amplified polymorphism，SRAP）的遗传多样性分析。运用NTSYS-pc 2.1软件计算17份柱花草的遗传相似系数，17条CDDP引物以及26对SRAP引物分别扩增出92条和177条带，其中多态性条带数分别为66条和111条，多态性比率分别为71.7%和62.7%，特异性条带各为13条和29条，特异性比率分别为14.1%和16.4%，种质间的遗传相似系数（GS）范围为0.54~0.90和0.60~0.94，平均遗传相似系数为0.76和0.79。按照非加权配对算术平均法基于CDDP标记和SRAP标记联合作17份柱花草的聚类图，在遗传相似系数为0.82时可将17份柱花草分成7个类群，聚类结果与农艺性状统计结果基本一致。本研究为今后辐射诱变柱花草育种提供一定的参考依据。

关键词: 柱花草, ⁶⁰Coγ射线, 农艺性状, CDDP, SRAP, 遗传多样性

CLC Number:

S335.21

LUO Ming-xin, LIU Feng-min, CHEN Ji-yan, CUI Jun-tao, ZHANG Wei-li. Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ[J]. Acta Agrestia Sinica, 2021, 29(9): 1992-2000.

罗铭欣, 刘凤民, 陈纪言, 崔均涛, 张伟丽. ⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析[J]. 草地学报, 2021, 29(9): 1992-2000.

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Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ

⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析

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