Acta Agrestia Sinica

›› 2010, Vol. 18 ›› Issue (2): 219-222.DOI: 10.11733/j.issn.1007-0435.2010.02.014

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Effect of Different Combinations and Concentrations of 2,4-D and 6-BA on Callus Induction and Differentiation of Medicago Sativa L.cv.Jinda

WANG Xiao-chun1, SHI Shang-li1, LIANG Hui-min2, XIA Yang3   

  1. 1. College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu province 730070, China;
    2. Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu province 212400, China;
    3. Shandong Provincial Acadey of Foresry, Jinan, Shangdong province 250014, China
  • Received:2009-01-09 Revised:2010-01-25 Online:2010-04-15 Published:2010-04-15

2,4-D和6-BA组合配比对金达苜蓿愈伤组织诱导与分化的影响

王晓春1, 师尚礼1, 梁慧敏2, 夏阳3   

  1. 1. 甘肃农业大学草业学院, 甘肃, 兰州, 730070;
    2. 江苏农林职业技术学院, 江苏, 句容, 212400;
    3. 山东省林业科学研究所, 山东, 济南, 250014
  • 通讯作者: 梁慧敏,E-mail:lianghuimin0218@126.com
  • 作者简介:王晓春(1982- ),女,汉族,硕士研究生,研究方向为牧草育种及种质资源.E-mail:xiaochun54@163.com
  • 基金资助:
    “十一五”国家科技支撑计划项目(2006BAD01A19);“十一五”国家科技支撑计划项目(2006BAD04A04-03);“十一五”国家科技支撑计划(2007BAD52B06)项目;农业部公益性行业(农业)科研专项(nyhyzx07-022)资助

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Abstract: The upside and downside of hypocotyls and cotyledon of Medicago Sativa L.cv.Jinda were used to study the effect of different concentrations of 2,4-D and 6-BA on the callus induction and embryogenic callus,embryoid differentiation.The results showed that the upside and downside of hypocotyls,cotyledon were good explants for Jinda.The best combinations and concentrations of 2,4-D、6-BA is 8/0.2 in inducing embryoid medium MSO.The best medium for plant development was MSO+30 g/L sucrose + 6 g/L agar.The goal of this research is to establish an effective regeneration system of Medicago Sativa L.cv.Jinda.by tissue culture and it has important significance for establishing genetic transformation system.

Key words: Medicago Sativa L., Callus, Embryoid, Induction, Differentiation

摘要： 为了建立一个高效的金达苜蓿(Medicago sativa L. cv. Jinda)组织培养再生体系,我们以金达苜蓿无菌苗的胚轴上段、下段、子叶为外植体,进行了2,4-D和6-BA不同组合配比对愈伤组织诱导和胚性愈伤组织及胚状体分化的影响,这对其以后建立遗传转化体系具有重要的意义。结果表明:胚轴上、下段和子叶是很好的诱导愈伤组织的外植体;在MSO胚状体诱导培养基上,分化出胚性愈伤组织的最佳2,4-D、6-BA剂量配比为8:0.2,成苗培养基为MSO+30 g/L蔗糖+6 g/L琼脂。

关键词: 金达苜蓿, 愈伤组织, 胚状体, 诱导, 分化

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