Acta Agrestia Sinica

›› 2011, Vol. 19 ›› Issue (2): 273-276.DOI: 10.11733/j.issn.1007-0435.2011.02.017

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Effect of Wilting and Adding PFJ on Fermentation Quality of Hybrid Pennisetum(Pennisetum americanum×P.purpureum) Silage

ZHENG Dan2, XIATIAO Ya-jing3, SHAO Tao1   

  1. 1. Laboratory of Ensiling and Processing of Grass, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
    2. Department of Biotechnology, Horticulture, Pharmaceutical Engineering, Tai zhou College of Nanjing Normal University, Taizhou, Jiangsu Province, 225300, China;
    3. Laboratory of Animal Feed Science, Division of Animal Science, Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
  • Received:2009-05-25 Revised:2010-11-29 Online:2011-04-15 Published:2011-04-15

凋萎和添加绿汁发酵液对杂交狼尾草青贮发酵品质的影响

郑丹2, 下条雅敬3, 邵涛1   

  1. 1. 南京农业大学动物科学技术学院, 饲草调制与加工研究室, 江苏, 南京, 210095;
    2. 南京师范大学泰州学院生物技术、园艺、制药工程系, 江苏, 泰州, 225300;
    3. 九州大学生物资源与环境学部动物饲料生产与利用研究室, 日本, 福冈, 812-8581
  • 通讯作者: 邵涛,E-mail:taoshaolan@yahoo.com.cn
  • 作者简介:郑丹(1981- ),女,黑龙江鹤岗人,硕士,助教,主要从事饲草调制与加工研究,E-mail:zhengdan-1122@163.com
  • 基金资助:
    国家自然科学基金面上项目(30771530)资助

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Abstract: This study was carried out to evaluate the effects of wilted,pre-fermented juice(PFJ) of epiphytic lactic acid bacteria,and wilted+PFJ on the fermentation qualities and residual water soluble carbohydrate(WSC) of Hybrid Pennisetum(Pennisetum americanum×P.purpureum) silage.The treatments were as follows: control(C),wilted(W),PFJ addition,wilted+PFJ on fresh weight basis of Hybrid Pennisetum,respectively.Based on results,control had low pH(4.09) and high lactic acid content(79.80 g/kg DM),and fermentation was clearly dominated by lactic acid bacteria.W treatment showed significantly(P<0.05) higher pH and significantly(P<0.05) lower lactic acid than the control indicating that although lactic acid bacteria have a relatively high tolerance to low moisture conditions,and lactic acid fermentation was inhibited in W treatment silage.Ammonia-N /total nitrogen(AN/TN),butyric acid,propionic acid,total volatile fatty acid(VFAs) and pH decreased or decreased significantly,whereas lactic acid /acetic acid,lactic acid,WSC increased significantly in both PFJ and W+PFJ treatments compared with control(P<0.05).These indicated that PFJ and W+PFJ treatments not only inhibited the activity of clostridia and other undesired microorganisms,but decreased protein degradation.However,they also stimulated homofermentative lactic acid bacteria activity,which decreased the loss of WSC,greatly increased the efficiency of WSC utilization by lactic acid bacteria,and further improved the fermentation quality.Wilted+PFJ treatment is best for improving the fermentation quality of Hybrid Pennisetum silage in this experiment.

Key words: Hybrid Pennisetum, Wilted, Pre-Fermented Juice, Fermentation Quality, Water Soluble Carbohydrate

摘要： 为探讨凋萎、添加绿汁发酵液、凋萎+绿汁发酵液处理对杂交狼尾草(Pennisetum americanum×P.purpureum)发酵品质与水溶性碳水化合物的影响。试验设对照组(Control)、凋萎组(W)、绿汁发酵液组(PFJ)及凋萎(W)+绿汁发酵液组(PFJ),青贮30天后开窖取样分析。结果表明:Control组有较低的pH(4.09)和较高的乳酸含量(79.80 g·kg-1DM),青贮主要是受乳酸发酵支配;W组的pH值显著高于Control组(P<0.05),而乳酸含量显著低于Control组(P<0.05),说明尽管乳酸菌对较干燥的环境有一定的忍受力,但是凋萎在一定程度上抑制了乳酸菌的活性。PFJ组和W+PFJ组pH值、氨态氮/总氮、丙酸、丁酸、乙酸、总挥发性脂肪酸含量低于或显著低于Control组,而乳酸、水溶性碳水化合物含量及乳酸/乙酸又显著高于Control组(P<0.05),表明添加绿汁发酵液后促进同质乳酸菌的发酵,有效地抑制了丁酸菌及其他有害微生物活性,减少了蛋白质的分解,提高了乳酸菌对水溶性碳水化合物的利用效率及杂交狼尾草青贮发酵品质。综合考虑,建议凋萎后添加绿汁发酵液处理效果更好。

关键词: 杂交狼尾草, 凋萎, 绿汁发酵液, 发酵品质, 水溶性碳水化合物

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