Zymogram Analysis of Different Sexual Buchloe dactyloides

doi:10.11733/j.issn.1007-0435.2012.03.023

Acta Agrestia Sinica ›› 2012, Vol. 20 ›› Issue (3): 530-535.DOI: 10.11733/j.issn.1007-0435.2012.03.023

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Zymogram Analysis of Different Sexual Buchloe dactyloides

LI Yong-xiang¹, WANG Xian-guo², CONG Li-li¹, YANG Chun-hua¹

1. Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China;
2. Institute of Grassland Science, China Agricultural University, Beijing 100193, China

Received:2011-10-25 Revised:2011-12-22 Online:2012-06-15 Published:2012-07-05

不同性别野牛草同工酶酶谱分析

李永祥¹, 王显国², 丛丽丽¹, 杨春华¹

1. 四川农业大学草业科学系, 四川雅安 625014;
2. 中国农业大学草业科学系, 北京 100193

通讯作者: 杨春华,E-mail:ychh@sicu.edu.cn
作者简介:李永祥(1985- ),男,山东济南人,硕士研究生,主要从事野牛草性别鉴定的研究,E-mail:493871113@163.com
基金资助:
国家自然科学基金项目(30800799)资助

Abstract

Abstract: Superoxide dismutase (SOD), peroxidase (POD) and polyphenol oxidase (PPO) isozymes of leaves and spikes from different sexual Buchloe dactyloides (male, female or monoecious) specimens were analyzed using polyacrylamide gel electrophoresis. Results showed that the POD and PPO zymogram of spikes from male plants had distinct bands, Rf0.12 and Rf0.15, respectively; the PPO zymogram of both leaves from female plants and spikes from monoecious plant had a special band, Rf0.12. Therefore, foliar POD and PPO zymogram can be used for sex determination during early growth stages of Buchloe dactyloides.

Key words: Buchloe dactyloides, Determination of sex, Isoenzyme

摘要： 利用聚丙烯酰胺垂直板凝胶电泳技术对3种性别野牛草(Buchloe dactyloides)的幼叶和幼穗的过氧化物酶(POD)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)同工酶酶谱进行分析,以期通过酶谱的差异表达对野牛草进行早期性别鉴定。结果表明:雄株野牛草幼穗的POD和PPO同工酶各有1条特异条带Rf0.12和Rf0.15;雌雄同株的幼穗PPO同工酶酶谱中有1条特异条带Rf0.12;雌株的叶片PPO同工酶酶谱有Rf0.12的特异性条带。通过分析上述3种同工酶的酶谱发现,叶片的POD和PPO同工酶酶谱可以用来对野牛草进行早期性别鉴定。

关键词: 野牛草, 性别鉴定, 同工酶

CLC Number:

Q949
S543.9

LI Yong-xiang, WANG Xian-guo, CONG Li-li, YANG Chun-hua. Zymogram Analysis of Different Sexual Buchloe dactyloides [J]. Acta Agrestia Sinica, 2012, 20(3): 530-535.

李永祥, 王显国, 丛丽丽, 杨春华. 不同性别野牛草同工酶酶谱分析[J]. 草地学报, 2012, 20(3): 530-535.

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References

[1] Quinn J A, Engel J L. Life-history strategies and sex ratios for a cultivar and wild population of Buchloe dactyloides (Nutt.) Engelm [J].American Journal Botany,1986,73(6):874-881
[2] 朱铁霞,胡自治,刘自学,等.北京地区3种暖季型草坪蒸散量的研究[J].草原与草坪,2009(1):71-73
[3] 何军,胡自治,刘自学,等.灌溉与草坪地下植物量关系的研究[J].草原与草坪,2005(1):46-49
[4] Johnson P G, Riordan T P, Johnson-Cicalese J. Low-mowing tolerance in buffalo grass [J].Crop Science,2000,40(5):1339-1343
[5] Basaglia F, Marhetti M G, Salvatorelli G. Genetic, developmental and comparative analysis of LDH, MDH and GPI isozyme in the sheeps head Bream (Diplodus puntazzo Gm.) [J].Comparative Biochemistry and Physiology-Part B: Comparative Biochemistry,1990,96(2):257-266
[6] Siegel S M, Chen J, Kottenmeier W, et al. Reduction in peroxidaes in Cucumis, Brassica and other seedlings cultured in saline waters [J].Phytochemistry,1982,21(3):539-542
[7] Karpinska B, Karlsson M, Schinkel H, et al. A novel super oxide dismutase with a high isoelectric point in higher plant. Expression, regulation and protein localization [J]. Plant Physiology,2001,126(4):1668-1677
[8] Caliskan M, Yavuz A, Aktoklu E. Isoenzyme variation in two allopatric populations of Hedysarum pogonocarpum Bioss.(Fabaceae), a Turkish endemic[J]. Biochemical Systematics and Ecology,2006,34(2):119-129
[9] Chenian y M, Ebrahimzadeh H, Salimi A, et al. Isozyme variation in some populations of wild diploid wheats in Iran[J]. Biochemical Systematics and Ecology,2007,35(6):363-371
[10] 王隆华,李谷英,沈曾佑.垂柳(Salix babylonical L.)雌雄植株的早期鉴别[J].华东师范大学学报:自然科学版,1989(2):100-104
[11] 杜希华,范志强,李君,等.过氧化物酶同工酶与银杏性别及年龄的关系[J].山东林业科技,1997(4):4-6
[12] 赵云云,田汝岭,刘捷平,等.雌雄构树过氧化物同工酶的比较研究[J].首都示范大学学报:自然科学版,1996(2):84-87
[13] 栾晓燕,陈怡,杜维广,等.不同抗性大豆品种感染SMV后过氧化物酶、多酚氧化酶、超氧化物歧化酶的变化分析[J].大豆科学,2001,20(3):200-203
[14] 郭尧君.蛋白电泳实验技术[M].北京:科学出版社,2005:90-110
[15] 罗广华,王爱国.植物SOD的凝胶电泳及活性显示[J].植物生理学通讯,1983,19(6):44
[16] 程昕昕,耿广汉,刘正.玉米杂种一代的酯酶酶谱分析[J].种子,2007,26(4):30-31
[17] Kuhus L J, Fret z T A. Distinguishing rose cultivars by polyacrylamide gel electrophoresis. I. Extraction and storage of protein and active enzymes from rose leaves [J]. Journal of the American Society for Horticultural Science,1978,103(4):503-508
[18] 苏应娟,刘启宏. 湖北八角莲属植物过氧化物酶同工酶分析[J].武汉植物学研究,1994,12(1):44-47
[19] 薛俊杰,张震云,乔天平,等.几种木本豆科植物的过氧化物酶和多酚氧化酶同工酶研究[J].山西农业大学学报,2000,20(1):55-57
[20] Alekhova T, Sofin A, Kobelkova T, et al. Sex linked differences in activity of enzymes in the blood of the urodle amphibian Pleurodeles waltl [J].Comparative Biochemistry and Physiology-Part A: Molecular & Integrative Physiology,2001,130(4):819-825
[21] Penel C L, Greppin H. Evolution of the auxin-oxidase and peroxidase activity during the spinach’s photo periodic induction and sexualistion [J]. Plant and Cell Physiology,1972,13(1):151-156
[22] 林伯年,沈德绪.杨梅雌雄株同工酶和酚类物质的鉴别[J],浙江农业大学学报,1995,21(1):22-26
[23] 张立平.雌雄异株葡萄的性别鉴定研究[J].植物学通报,1998,15(4):63-67
[24] 张学明.杨梅和银杏雌雄性的早期鉴别[J].经济林研究,1989,7(2):79-80
[25] 范双喜,杜新民.石刁柏性别表现与同工酶的关系[J].北京农学院学报,1995,10(2):44-48
[26] 艾辛,祝莉莉,舒理慧,等.黄瓜植株性别表现与3种氧化酶同工酶的关系[J].武汉植物学研究,2000,18(3):184-188
[27] 李惠敏,黄夕洋,高成伟,等,罗汉果雌雄株同工酶性别鉴定研究[J].广西植物,2007,25(5):792-795
[28] 陈中海.番木瓜性别的生化标记与分子标记研究.福州:福建农林大学,2001:20-25
[29] Huff D R, Wu Lin. Sex expression in buffalograss under different environments [J]. Crop Science,1987,27(4):623-626
[30] Wu Lin, A H Harvindi, V A Gibeault. Obeservations on buffalograss sexual characteristic and potential for seed production improvement [J].HortScience,1984,19(4):505-506

Zymogram Analysis of Different Sexual Buchloe dactyloides

不同性别野牛草同工酶酶谱分析

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