Analysis of Genetic Diversity and Population Structure in Medicago Germplasm by SSR Markers

doi:10.11733/j.issn.1007-0435.2013.04.020

›› 2013, Vol. 21 ›› Issue (4): 759-768.DOI: 10.11733/j.issn.1007-0435.2013.04.020

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Analysis of Genetic Diversity and Population Structure in Medicago Germplasm by SSR Markers

CHEN Fei, WEI Zhen-wu, LI Wei-min, PAN Ling, ZHENG Xi, LIU Qian, ZHANG Dong, TU De-peng

College of Animal Science & Technique, Institute of Forage and Grassland Science, Yangzhou Unicersity, Yangzhou, Jiangsu Province 225009, China

Received:2013-01-16 Revised:2013-03-12 Online:2013-08-15 Published:2013-08-14

基于SSR标记的苜蓿种质资源遗传多样性与群体结构分析

陈斐, 魏臻武, 李伟民, 潘玲, 郑曦, 刘倩, 张栋, 屠德鹏

扬州大学动物科学与技术学院扬州大学草业科学研究所, 江苏扬州 225009

通讯作者: 魏臻武
作者简介:陈斐(1988- ),女,甘肃兰州人,硕士研究生,研究方向为牧草遗传育种,E-mail: chenfei_198807@yahoo.com.cn
基金资助:
国家自然科学基金项目(30972136);江苏省科技支撑计划 (BE2012340);江苏省六大人才高峰资助项目资助

Abstract

Abstract: Understanding the genetic diversity and population structure is a prerequisite for the association mapping of complex traits in a target population. Genetic diversity and population structure of 82 alfalfa materials were evaluated using 16 polymorphic SSR markers screened from a total of 140 SSR primer pairs. Results showed that a total of 190 alleles were amplified using 16 SSR primer pairs, with an average of 11.88 alleles per primer pair, the polymorphic rate averaged 83.05%, ranging from 66.67%~100%. The genetic diversity averaged 0.2577, ranging from 0.1477~0.3637, and the PIC averaged 0.5501, ranging from 0.0079~0.9432. Results indicated that △K had a maximum value when K=5, namely, these 82 alfalfa materials could be divided into 5 groups. 75.61% Medicago germplasm were with single genetic components, the rest 24.39% were with mixed genetic components. The genetic diversities of Medicago germplasm were abundant and the division of population structure was not completely related to germplasm source.

Key words: Alfalfa (Medicago spp.), Germplasm resources, SSR markers, Genetic diversity, Population structure

摘要： 种质资源遗传多样性和群体结构的分析是进行复杂性状关联分析的前提条件。从140 对SSR引物中筛选到16 对多态性引物,对82 份苜蓿(Medicago spp.)材料进行遗传多样性与群体结构分析。结果表明:16 对SSR引物共检测出190个等位变异,平均11.88个;多态性比率66.67%~100%,平均为83.05%;基因多样性为0.1447~0.3637,平均为0.2577;PIC为0.0079~0.9432,平均为0.5501。群体结构分析表明,当K=5时ΔK最大,即82 份苜蓿材料可划分为5个类群,其中75.61%的种质遗传组分相对比较单一,24.39%的种质遗传背景比较复杂。苜蓿种质资源遗传多样性丰富,群体结构的划分与种质的来源不完全相关。

关键词: 苜蓿, 种质资源, SSR标记, 遗传多样性, 群体结构

CLC Number:

S603.6

CHEN Fei, WEI Zhen-wu, LI Wei-min, PAN Ling, ZHENG Xi, LIU Qian, ZHANG Dong, TU De-peng. Analysis of Genetic Diversity and Population Structure in Medicago Germplasm by SSR Markers[J]. , 2013, 21(4): 759-768.

陈斐, 魏臻武, 李伟民, 潘玲, 郑曦, 刘倩, 张栋, 屠德鹏. 基于SSR标记的苜蓿种质资源遗传多样性与群体结构分析[J]. , 2013, 21(4): 759-768.

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Analysis of Genetic Diversity and Population Structure in Medicago Germplasm by SSR Markers

基于SSR标记的苜蓿种质资源遗传多样性与群体结构分析

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