Producing Laccase-characteristics of Marasmius tricolor Isolated from the Soil of Alpine Meadow

doi:10.11733/j.issn.1007-0435.2014.05.026

Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (5): 1080-1085.DOI: 10.11733/j.issn.1007-0435.2014.05.026

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Producing Laccase-characteristics of Marasmius tricolor Isolated from the Soil of Alpine Meadow

LU Guang-xin¹, CHEN Xiu-rong², WANG Jun-bang³, WU Chu⁴, WEI Wei-dong¹, YANG Fan¹, WEN Xiao-cheng¹

1. Agriculture and Animal husbandry College, Qinghai University, Xining, Qinghai Province 810016, China;
2. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
3. Institute of Geographic Sciences and Nature Resources Research, CAS, Beijing 100094, China;
4. College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei Province 434025, China

Received:2013-12-30 Revised:2014-03-17 Online:2014-10-15 Published:2014-09-30

高寒草地土壤三色小皮伞菌产漆酶特性的研究

芦光新¹, 陈秀蓉², 王军邦³, 吴楚⁴, 魏卫东¹, 杨帆¹, 温小成¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 甘肃农业大学草业学院, 甘肃兰州 730070;
3. 中国科学院地理科学与资源研究所生态系统网络观测与横拟重点实验室, 北京 100101;
4. 长江大学园艺园林学院, 湖北荆州 434025

通讯作者: 陈秀蓉，王军邦
作者简介:芦光新（1974-），男，青海湟中人，博士，教授，主要从事草地微生物多样性及功能利用研究，E-mail：lugx74@qq.com；
基金资助:
国家自然科学基金“青藏高原草地耐低温纤维素分解真菌多样性研究”（41261064）；青海大学高层次人才基金“产漆酶真菌的筛选及多酶系菌群的构建”（2012-QGC-9）资助

Abstract

Abstract:

A fungus strain belonging to Marasmius tricolor has been isolated and selected from the soil of alpine meadow located in eastern Qilian Mountains. To full understand the characteristics of the fungus producing the laccase, three substrates (i.e., guaiacol, caechol, and o-benzyl aniline) were used to induce the activity of laccase, using substrate spectrophotometry and liquid rotating methods. The temporal order and sizes that the oxidation zones occurred on guaiacol-PDA culture media were analyzed to investigate the relative rates that the fungus produced laccase. The results showed that Marasmius tricolor produced oxidation zones cultured on the media that contained guaiacol, caechol, and o-benzyl aniline, respectively, but the colors and diameter sizes of the oxidation zones and fungus community diameters differed from different media. The diameters of the oxidation zones and fungus community were the greatest when the fungus was cultured on the medium that contained guaiacol. The fungus showed the greatest activity producing laccase. Guaiacol might induce its hypha to secrete laccases at the early stage of its growth. Laccase activity was the highest when guaiacol was substrate in the reaction system. Laccase produced by the fungus showed the greatest activity under the condition of 30℃ and pH 4.5. The enzyme also showed higher activity under the condition of 0~45℃, pH 4.0~6.5.

Key words: Alpine meadow, Laccase, Marasmius tricolor

摘要：

以1株三色小皮伞菌（Marasmius tricolor）（编号310b）为供试菌株，采用底物显色法和液体摇瓶发酵法，研究了供试菌株Marasmius tricolor 310b在3种底物（愈创木酚、邻苯二酚和邻苯甲苯胺）选择性培养基上产漆酶的相对酶活力，在愈创木酚-PDA培养基上的相对产酶速度，以愈创木酚为底物催化液体发酵粗酶液的酶活力以及粗酶液酶学性质。结果表明：供试菌株Marasmius tricolor 310b在含有愈创木酚、邻苯二酚和邻苯甲苯胺等3种底物的选择性培养基上能够生长且产生氧化带，在不同底物的选择性培养基上氧化带的颜色、直径以及菌落大小不尽相同，在愈创木酚选择性培养基上产生的氧化带直径和菌落直径最大。愈创木酚能够在供试菌株生长早期诱导菌丝分泌漆酶；以愈创木酚为底物测得菌株液体发酵粗酶液的漆酶活力最高。供试菌株分泌的漆酶在30℃、pH=4.5时酶的催化活性达到最大值，0～45℃、pH 4.0～6.5范围内保持较高的酶活力。

关键词: 高寒草地, 漆酶, 三色小皮伞菌

CLC Number:

S154.3
Q55

LU Guang-xin, CHEN Xiu-rong, WANG Jun-bang, WU Chu, WEI Wei-dong, YANG Fan, WEN Xiao-cheng. Producing Laccase-characteristics of Marasmius tricolor Isolated from the Soil of Alpine Meadow[J]. Acta Agrestia Sinica, 2014, 22(5): 1080-1085.

芦光新, 陈秀蓉, 王军邦, 吴楚, 魏卫东, 杨帆, 温小成. 高寒草地土壤三色小皮伞菌产漆酶特性的研究[J]. 草地学报, 2014, 22(5): 1080-1085.

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Producing Laccase-characteristics of Marasmius tricolor Isolated from the Soil of Alpine Meadow

高寒草地土壤三色小皮伞菌产漆酶特性的研究

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