Cultivars (Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers

doi:10.11733/j.issn.1007-0435.2015.01.024

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (1): 151-155.DOI: 10.11733/j.issn.1007-0435.2015.01.024

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Cultivars (Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers

LEI Yun-ting¹, ZHAO Yan-yan^1,2, YU Feng^1,2, LI Yuan^1,2, DOU Quan-wen¹

1. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810001, Chian;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2014-02-27 Revised:2014-04-15 Online:2015-02-15 Published:2015-01-31

利用基因组SSR分子标记对老芒麦品种(种质)鉴别和品种纯度鉴定

雷云霆¹, 赵闫闫^1,2, 喻凤^1,2, 李媛^1,2, 窦全文¹

1. 中国科学院西北高原生物研究所, 青海西宁 810001;
2. 中国科学院大学, 北京 100049

通讯作者: 窦全文
作者简介:雷云霆(1985-),男,四川阆中人,硕士研究生,研究方向为植物遗传育种,E-mail:leiyunting@163.com
基金资助:
青海省科技厅科技促进新农村建设计划项目(2013-N-515);中科院"西部之光"联合学者人才培养计划项目资助

Abstract

Abstract:

Molecular fingerprinting maps of 3 Elymus sibiricus cultivars and 7 germplasms were carried out using 6 simple sequences repeat (SSR) markers developed from the genomic DNA of E. sibiricus. A total of 21 polymorphic SSR fragments were detected in all the tested materials, and 2 to 5 alleles were detected by each pair of SSR primer with an average of 3.5. All the 3 cultivars and 7 germplasms could be well distinguished by the fingerprinting maps constructed by 3 pairs of core primers. In addition, seed purity assessment was conducted in cultivars ‘Tongde' and ‘Duoye' with 3 pairs of SSR primers. It showed that seed purity and the polymorphism of SSR alleles could be effectively identified. Moreover, it revealed that ‘Tongde', a domesticated cultivar, had much lower purity and higher polymorphic alleles than ‘Duoye', a breeding cultivar. The SSR markers used in this study can be a powerful tool for cultivars or germplasms identification, seeds purity assessment in E. sibiricus.

Key words: Elymus sibiricus, Genomic SSR markers, Cultivar discrimination, Cultivar purity assessment

摘要：

利用从老芒麦(Elymus sibiricus)自身基因组中开发出来的6对多态性SSR引物,对3个老芒麦牧草品种和7个种质进行分子指纹图谱鉴别研究.结果表明:6对SSR引物在这10个材料中共检测出21个多态性SSR标记片段,每对引物可以检测到2~5个数目不等的片段,平均为3.5个.6对引物中3对核心引物组合可以将这10个材料进行有效鉴别.进一步选用3对引物对2个老芒麦品种进行纯度鉴定,表明SSR标记可以对品种群体中具有变异位点的个体进行有效鉴定,同时揭示野生栽培品种‘同德’老芒麦比育成品种‘多叶'老芒麦具有较高的遗传变异度.

关键词: 老芒麦, 基因组SSR分子标记, 品种(种质)鉴别, 纯度鉴定

CLC Number:

S324

LEI Yun-ting, ZHAO Yan-yan, YU Feng, LI Yuan, DOU Quan-wen. Cultivars (Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers[J]. Acta Agrestia Sinica, 2015, 23(1): 151-155.

雷云霆, 赵闫闫, 喻凤, 李媛, 窦全文. 利用基因组SSR分子标记对老芒麦品种(种质)鉴别和品种纯度鉴定[J]. 草地学报, 2015, 23(1): 151-155.

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Cultivars (Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers

利用基因组SSR分子标记对老芒麦品种(种质)鉴别和品种纯度鉴定

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