Influence of Medicinal Plant Extracts on the Inhibition Activity of Embellisia astragli and the Seed Germination of Astragalus adsurgens

doi:10.11733/j.issn.1007-0435.2015.02.022

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (2): 366-371.DOI: 10.11733/j.issn.1007-0435.2015.02.022

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Influence of Medicinal Plant Extracts on the Inhibition Activity of Embellisia astragli and the Seed Germination of Astragalus adsurgens

ZENG Cui-yun^1,2, ZHU Xiao-yu², CUI Zhen¹, Li Yan-zhong¹

1. College of Pastoral Agriculture Science and Technology, Lanzhou University; Gansu Grassland Ecological, Research Institute, Lanzhou, Gansu Province 730020, China;
2. Gansu College of Traditional Chinese Medicine, Lanzhou, Gansu Province 730000, China

Received:2014-04-10 Revised:2014-06-13 Online:2015-04-15 Published:2015-03-25

药用植物提取液对沙打旺黄矮根腐病菌的抑菌活性及对其种子萌发的影响

曾翠云^1,2, 朱晓玉², 崔振¹, 李彦忠¹

1. 兰州大学草地农业科技学院农业部草地农业生态系统学重点开放实验室甘肃草原生态研究所, 甘肃兰州 730020;
2. 甘肃中医学院, 甘肃兰州 730000

通讯作者: 李彦忠
作者简介:曾翠云(1979-),女,甘肃白银人,博士研究生,主要从事药用植物和牧草植物病害研究工作,E-mail:zengcy1028@163.com
基金资助:
优秀博士学位获得者资助项目(201068);国家自然科学基金(31272496);公益性行业(农业)科研专项 (201303057);国家牧草产业技术体系(CFGRS);教育部重大科技项目 (313028)资助

Abstract

Abstract:

To provide the theory basis of botanical fungicide control on Embellisia astragli, the organic extracts from 15 kinds of medicinal plants, garlic, green potato, and the green epidermis of Walnut were tested in vitro for antifungal activity by growth speed method and concave slide method. Influence of seed germination was also tested using the plant extracts possessing the best inhibitory effect. The results showed that Saposhnikovia divaricata, Allium sativum and Juglans regia had the strongest antifungal activity on mycelium growth(P <0.05), the inhibition rates were all 100%. These extractions had significant inhibition function against the germination of the spore, the inhibition rates were all above 85%. And the extractions also affected the seed germination of Astragalus adsurgens (P <0.05),the germination potential and germination rate were all less than 50%. Therefore, these extractions had the application prospect of fungicide control, but were unfavorable seed dressing

Key words: Medicinal plants, Ethanol extractions, Embellisia astragali, Antifungal activity.

摘要：

为给植物源杀菌剂防治沙打旺黄矮根腐病菌(Embellisia astragli)提供理论依据,采用生长速率法和凹玻片法测试15种药用植物以及大蒜(Allium sativum)、变绿马铃薯(Solanum tuberosum)和核桃青皮 (Juglans regia)3种植物提取液对沙打旺(Astragalus adsurgens)黄矮根腐病菌的离体抑菌活性的影响,并测定抑制效果最好的植物提取液对沙打旺种子萌发活性的影响.结果表明:防风(Saposhnikovia divaricate)、大蒜、核桃青皮3种植物提取液对沙打旺黄矮根腐病原菌具有显著的抑菌作用(P <0.05),抑菌率均达100%,对病原菌孢子萌发抑制力较强, 孢子萌发抑制率均达85%.防风、大蒜、核桃青皮3种植物提取液原液对沙打旺种子萌发具有显著抑制作用(P <0.05),种子的发芽势和发芽率均在50%以下.说明防风、大蒜和核桃青皮的提取液对沙打旺黄矮根腐病田间防治有一定应用前景,但在沙打旺播种时不宜使用其提取液进行药剂拌种.

关键词: 药用植物, 乙醇提取液, 沙打旺黄矮根腐病菌, 抑菌活性

CLC Number:

S435.4

ZENG Cui-yun, ZHU Xiao-yu, CUI Zhen, Li Yan-zhong. Influence of Medicinal Plant Extracts on the Inhibition Activity of Embellisia astragli and the Seed Germination of Astragalus adsurgens[J]. Acta Agrestia Sinica, 2015, 23(2): 366-371.

曾翠云, 朱晓玉, 崔振, 李彦忠. 药用植物提取液对沙打旺黄矮根腐病菌的抑菌活性及对其种子萌发的影响[J]. 草地学报, 2015, 23(2): 366-371.

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Influence of Medicinal Plant Extracts on the Inhibition Activity of Embellisia astragli and the Seed Germination of Astragalus adsurgens

药用植物提取液对沙打旺黄矮根腐病菌的抑菌活性及对其种子萌发的影响

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