Abstract:

To clarifying the effects of tannic acid on growth and reproduction of red and green-pea aphids, this paper reported on the survival rate, average progeny produced, and relative average daily growth rate of pea aphids under five different concentrations of tannic acid. The results showed that increasing concentrations of tannic acid have inhibitory effects on growth and reproduction of aphids. Furthermore, the inhibitory effects on green pea aphids were significantly greater than that on red pea aphid. After 12 days the survival rate and relative growth rate values of green pea aphids were 13.3±3.3%, 0.20 ±0.01%, respectively, when the concentration was 5 mg·L^-1, and those of red pea aphids were 20.0 ±5.8%, 0.19 ±0.00%, respectively; the difference with the control (CK) was significantly (P < 0.05). Also, at the concentration of 5 mg·L^-1, the litter size and rate of birth of green pea aphid was lowest (2.0/single females and 6.7 ±3.3%), and showed significant difference compared with control(P < 0.05). This report would provide guidance for the research of the biochemical resistance mechanismes of secondary metabolites to pea aphid.

Key words: Tannic acid, Pea aphid, Plant secondary metabolites, Growth and development, Reproduction

摘要：

为明确单宁酸对两种色型豌豆蚜生长发育及繁殖的影响，本研究采用豌豆蚜全纯人工饲料薄膜饲养技术，设置5组不同浓度的单宁酸，在适宜条件下分别饲养绿色型、红色型豌豆蚜，测定了豌豆蚜的存活率、产蚜量、相对日均体重增长率等指标。结果表明：单宁酸对两种生物型豌豆蚜的生长发育及繁殖有明显抑制作用，且对绿豌豆蚜的抑制作用明显大于红豌豆蚜。随着单宁酸浓度升高，两种色型豌豆蚜存活率、相对日均体重增长率均下降，5 mg·L^-1处理12天时绿色型豌豆蚜存活率、相对日均体重增长率分别为13.3±3.3%和0.20±0.01%，红色型分别为20.0±5.8%和0.19±0.00%，与CK间差异显著（P<0.05）；5 mg·L^-1时绿豌豆蚜产蚜量和产蚜率最低，分别为2.0±0.0头/单雌和6.7±3.3%，与CK间差异显著（P<0.05）。研究结果可为次生代谢物质抗豌豆蚜的生化机理研究提供理论依据和技术支持。

关键词: 单宁酸, 豌豆蚜, 植物次生代谢物质, 生长发育, 繁殖