Abstract: The objective of this study was to investigate the effects of different cultivars of photosensitive Sorghum-sudangrass hybrid on soil microecological environment in Jianghuai region. Four Sorghum Sudangrass hybrid cultivars were selected in this study:Monster(HN),Big Kahuna(DK),BJ0603(GDC) and Pa Kahuna(PK). Traditional methods were used to determine the physical and chemical properties of soil in different soil layers,enzyme activities were determined by kits in rhizosphere soil,and the diversity of bacterial community in rhizosphere soil was analyzed by Illumina MiSeq analysis. Results showed that the soil physical chemical properties and enzyme activities were significantly different (P<0.05) in soil of four cultivars;The Illumina Miseq analysis indicated that bacterial diversities were different in soil of different cultivars;The soil bacterial diversity of PK was higher than the other three cultivars;The redundancy analyses(RDA) analysis indicated that soil total nitrogen (TN) and soil organic matter (OM) were the most important factors to characterize the soil bacterial community composition. In summary,the cultivars of HN,PK,DK and GDC in Jiang-huai region showed cultivar differences in soil nutrient,bulk density,enzyme activity and bacterial community structure diversity,so different planting management schemes could be formulated according to the characteristics of different cultivars.

Key words: Photosensitive Sorghum Sudangrass hybrid, Jiang-huai region, Soil enzyme activities, Soil bacterial diversity, Illumina MiSeq analysis

摘要： 为了研究江淮地区不同品种光敏型高丹草（Sorghum-sudangrass hybrid）对土壤微生态环境的影响，本研究选取海牛Monster（HN）、帕卡Pa Kahuna（PK）、大卡Big Kahuna（DK）和高丹草BJ0603（GDC）4个品种，利用传统法测定不同土层土壤理化性质、使用试剂盒测定根际土壤酶活性，通过高通量测序法对根际土壤细菌群落多样性进行了分析。结果表明：各品种根部土壤理化性质、酶活性及微生物多样性具有显著的品种差异；PK根际土壤细菌多样性显著高于其他3个品种；根际土壤细菌群落多样性变化与土壤总氮（Total nitrogen，TN）及有机质（Organic matter，OM）含量变化关系较大。综上，不同品种土壤微生态环境差异显著，因此，根据不同品种特性制定引种及管理方案可以在提高产量和品质的基础上改良土壤，提高土地生产力。

关键词: 光敏型高丹草, 江淮地区, 土壤酶活性, 土壤细菌群落多样性, Illumina Miseq高通量测序