Effects of Nitrogen Forms on Plant Growth,Expression of Nitrate Transporter Gene MtNRT1.3,and Nitrogen Absorption in Medicago sativa L.

doi:10.11733/j.issn.1007-0435.2019.05.008

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1172-1180.DOI: 10.11733/j.issn.1007-0435.2019.05.008

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Effects of Nitrogen Forms on Plant Growth,Expression of Nitrate Transporter Gene MtNRT1.3,and Nitrogen Absorption in Medicago sativa L.

WANG Yu-qiang, SHEN Yu, QIAN jin, LIU Wen-tao, ZHAO Guo-qi, SUN Sheng-nan

College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, 225009, China

Received:2019-07-02 Revised:2019-07-25 Online:2019-10-15 Published:2019-11-09

不同形态氮肥对紫花苜蓿生长、硝酸盐转运蛋白基因MtNRT1.3表达及氮吸收的影响

王玉强, 沈宇, 钱进, 刘文涛, 赵国琦, 孙盛楠

扬州大学动物科学与技术学院, 江苏扬州 225009

通讯作者: 孙盛楠
作者简介:王玉强(1993-),男,汉族,江苏连云港人,硕士研究生,研究方向牧草与微生物互作关系,E-mail:741182243@qq.com
基金资助:
国家自然科学基金项目（31702176）资助

Abstract

Abstract: In order to improve utilization of different forms of nitrogen by leguminous forage,Alfalfa was used as experimental material to investigate the effects of different forms of nitrogen fertilizer on physiological characteristics,the expression of nitrate transporter gene MtNRT1.3 and nitrogen absorption through pot experiments. There were 4 treatments in the experiment,including control group (Con) without nitrogen application,ammonium nitrogen (NH₄Cl-T1),nitrate nitrogen (NaNO₃-T2) and mixed nitrogen (ammonium nitrogen,nitrate nitrogen 1:1 mixed-T3) were treated without nitrogen application. The total amount of nitrogen fertilizer applied was 250 mg (per 1 kg of soil) of pure nitrogen. The results showed that nitrogen application increased the nitrogen content of alfalfa. The application of various nitrogen fertilizers increased the expression of MtNRT1.3 gene in alfalfa roots,and the expression of MtNRT1.3 gene was positively correlated with soil ammonium nitrogen and nitrate nitrogen (P<0.01). Compared with ammonium nitrogen fertilizer,the application of nitrate nitrogen fertilizer can not only increase the nitrate nitrogen content of alfalfa,but also increase the ammonium nitrogen content of alfalfa. Compared with the application of nitrate nitrogen and ammonium nitrogen fertilizer alone,the mixed nitrogen fertilizer can increase the nitrogen content of alfalfa. The effect is the best;The application of nitrate nitrogen fertilizer was more beneficial to the aboveground biomass of alfalfa. Therefore,The ratio of ammonium nitrogen and nitrate nitrogen plays an important role in regulating plant growth,especially increasing the nitrate nitrogen content could make for nitrogen absorption in alfalfa.

Key words: Ammonium nitrogen, Nitrate nitrogen, Alfalfa, Nitrate transporter gene

摘要： 为了探讨豆科牧草对不同形态氮素的吸收，以紫花苜蓿（Medicago sativa L.）为试验材料，通过盆栽试验探究不同形态氮肥对紫花苜蓿生长、硝酸盐转运蛋白基因MtNRT1.3表达和氮吸收的影响。试验设有4个处理，分别为不施氮处理（对照组，Con）、施铵态氮（NH₄Cl—T1）、硝态氮（NaNO₃—T2）和混合氮（铵态氮、硝态氮1：1混合—T3），各形态氮肥施加总量为按纯氮250 mg（每1 kg土）。试验结果表明，施氮处理提高了紫花苜蓿中的氮含量，施加各种氮肥均提高了紫花苜蓿根中MtNRT1.3基因的表达量，且该基因的表达量与土壤铵态氮和硝态氮呈正相关性（P<0.01）。相比于铵态氮肥，施加硝态氮肥不但可增加植株中硝态氮含量，而且能提高植株铵态氮含量；相比于单施硝态氮和铵态氮肥，混合氮肥对提高植株氮含量效果最好；施加硝态氮肥更有利于紫花苜蓿地上部分生物量的累积。因此，对紫花苜蓿施加氮肥应重视铵态氮和硝态氮的比例，增加硝态氮的比例更有利于紫花苜蓿的生长和对氮素的吸收。

关键词: 铵态氮, 硝态氮, 紫花苜蓿, 硝酸盐转运蛋白基因

CLC Number:

S541

WANG Yu-qiang, SHEN Yu, QIAN jin, LIU Wen-tao, ZHAO Guo-qi, SUN Sheng-nan. Effects of Nitrogen Forms on Plant Growth,Expression of Nitrate Transporter Gene MtNRT1.3,and Nitrogen Absorption in Medicago sativa L.[J]. Acta Agrestia Sinica, 2019, 27(5): 1172-1180.

王玉强, 沈宇, 钱进, 刘文涛, 赵国琦, 孙盛楠. 不同形态氮肥对紫花苜蓿生长、硝酸盐转运蛋白基因MtNRT1.3表达及氮吸收的影响[J]. 草地学报, 2019, 27(5): 1172-1180.

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References

[1] 伦寿,景陵. 蔬菜营养与施肥技术[M]. 中国农业出版社,2002:60-72
[2] Britto D T,Kronzucker H J. NH₄⁺ toxicity in higher plants:a critical review[Review] [J]. Journal of Plant Physiology,2002,159(6):567-584
[3] Roosta H R,Sajjadinia A,Rahimi A,et al. Responses of cucumber plant to NH₄⁺ and NO₃^- nutrition:the relative addition rate technique vs. cultivation at constant nitrogen concentration[J]. Scientia Horticulturae,2009,121(4):397-403
[4] Caicedo J R,Steen NPVD,Arce O,et al. Effect of total ammonia nitrogen concentration and pH on growth rates of duckweed (Spirodela polyrrhiza)[J]. Water Research,2000,34(15):3829-3835
[5] 邹春琴,范晓云,石荣丽,等. 铵态氮和硝态氮对旱稻、水稻生长及铁营养状况的影响[J]. 中国农业大学学报,2007,12(4):45-49
[6] 苗艳芳,李生秀,徐晓峰,等. 冬小麦对铵态氮和硝态氮的响应[J]. 土壤学报,2014,3(3):564-574
[7] Smiciklas K D. Role of Nitrogen Form in Determining Yield of Field-Grown Maize[J]. Crop Science,1992,32(5):1220-1225
[8] Zhang H,Forde B G. Regulation of Arabidopsis root development by nitrate availability[J]. Journal of experimental botany,2000,51(342):51-59
[9] Linkohr B I,Williamson L C,Fitter A H,et al. Nitrate and phosphate availability and distribution have different effects on root system architecture of Arabidopsis[J]. The Plant Journal,2002,29(6):751-760
[10] Glass A D,Siddiqi M Y,Ruth T J,et al. Studies of the Uptake of Nitrate in Barley:II. Energetics[J]. Plant Physiology,1992,99(2):456-463
[11] Siddiqi M Y,Glass A D,Ruth TJ,et al. Studies of the Regulation of Nitrate Influx by Barley Seedlings Using NO(3)[J]. Plant Physiology,1989,90(3):806-813
[12] Cécile S,Jean-Christophe B,Emilie I,et al. Nitrate efflux at the root plasma membrane:identification of an Arabidopsis excretion transporter[J]. Plant Cell,2007,19(11):3760-3777
[13] Miller A J,Fan X,Orsel M,et al. Nitrate transport and signalling[J]. Journal of Experimental Botany,2007,58(9):2297-2306
[14] Zifarelli G,Pusch M. CLC transport proteins in plants[J]. Febs Letters,2010,584(10):2122-2127
[15] Forde B G. Nitrate transporters in plants:structure,function and regulation[J]. Biochim Biophys Acta,2000,1465(1):219-235
[16] Yi-Fang T,Chi-Chou C,Chyn-Bey T,et al. Nitrate transporters and peptide transporters[J]. Febs Letters,2007,581(12):2290-2300
[17] Tsay Y F,Schroeder J I,Feldmann K A, et al. The herbicide sensitivity gene CHL1 of arabidopsis encodes a nitrate-inducible nitrate transporter[J]. Cell,1993,72(5):705-713
[18] Huang N C,Liu K H,Lo HJ,et al. Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake[J]. Plant Cell,1999,11(8):1381-1392
[19] Chi-Chou C,Choun-Sea L,An-Ping H,et al. Mutation of a nitrate transporter,AtNRT1:4,results in a reduced petiole nitrate content and altered leaf development[J]. Plant & Cell Physiology,2004,45(9):1139-1148
[20] Shan-Hua L,Hui-Fen K,Geneviève C,et al. Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport[J]. Plant Cell,2008,20(9):2514-2528
[21] Anabel A,Hua L S,Fang T Y. Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development[J]. Plant Cell,2008,20(12):3289-3299
[22] Koh S,Stacey G,Yu S M,et al. Cloning and Functional Characterization of a Constitutively Expressed Nitrate Transporter Gene,OsNRT1,from Rice[J]. Plant Physiology,2000,122(2):379-388
[23] Zhou J J,Theodoulou F L,Muldin I,et al. Cloning and functional characterization of a Brassica napus transporter that is able to transport nitrate and histidine[J]. Journal of Biological Chemistry,1998,273(20):12017-12023
[24] Huang N-C,Chiang C-S,Crawford N M,et al. CHL1 encodes a component of the low-affinity nitrate uptake system in Arabidopsis and shows cell type-specific expression in roots[J]. The Plant Cell,1996,8(12):2183-2191
[25] Walch-Liu P,Liu L-H,Remans T,et al. Evidence that L-glutamate can act as an exogenous signal to modulate root growth and branching in Arabidopsis thaliana[J]. Plant and Cell Physiology,2006,47(8):1045-1057
[26] Walch-Liu P,Forde B G. Nitrate signalling mediated by the NRT1. 1 nitrate transporter antagonises l-glutamate-induced changes in root architecture[J]. The Plant Journal,2008,54(5):820-828
[27] Ho C-H,Lin S-H,Hu H-C,et al. CHL1 functions as a nitrate sensor in plants[J]. Cell,2009,138(6):1184-1194
[28] Krouk G,Lacombe B,Bielach A, et al. Nitrate-regulated auxin transport by NRT1. 1 defines a mechanism for nutrient sensing in plants[J]. Developmental cell,2010,18(6):927-937
[29] Martin C,Florian F. De novo organ formation from differentiated cells:root nodule organogenesis[J]. Science Signaling,2008,1(49):431-440
[30] Yokoyama T,Kodama N,Aoshima H,et al. Cloning of a cDNA for a constitutive NRT1 transporter from soybean and comparison of gene expression of soybean NRT1 transporters[J]. BBA-Gene Structure and Expression,2001,1518(1):79-86
[31] Christian J,Sandrine R,Sandra F, et al. Adaptation of Medicago truncatula to nitrogen limitation is modulated via local and systemic nodule developmental responses[J]. New Phytologist,2010,185(3):817-828
[32] Omrane S,Ferrarini A,D'Apuzzo E,et al. Symbiotic competence in Lotus japonicus is affected by plant nitrogen status:transcriptomic identification of genes affected by a new signalling pathway[J]. New Phytologist,2009,183(2):380-394
[33] Paven MCM,Viau L,Hamon A, et al. Characterization of a dual-affinity nitrate transporter MtNRT1.3 in the model legume Medicago truncatula[J]. Journal of Experimental Botany,2012,62(15):5595
[34] Morère-Le Paven M-C,Viau L,Hamon A,et al. Characterization of a dual-affinity nitrate transporter MtNRT1. 3 in the model legume Medicago truncatula[J]. Journal of experimental botany,2011,62(15):5595-5605
[35] 孙建光,张燕春,徐晶,等. 高效固氮芽孢杆菌筛选及其生物学特性[J]. 中国农业科学,2009,42(6):2043-2051
[36] 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社,2002:67-96
[37] 苗艳芳,吕静霞,李生秀,等. 铵态氮肥和硝态氮肥施入时期对小麦增产的影响[J]. 水土保持学报,2014,28(4):91-96
[38] Schroder,Zhang H,Desta K,et al. Soil acidification from long-term use of nitrogen fertilizers on winter wheat[J]. Soil Science Society of America Journal,2011,75(3):957-964
[39] Guo J H,Liu X J,Zhang Y,et al. Significant acidification in major Chinese croplands[J]. Science,2010,327(5968):1008-1010
[40] 王西娜,王朝辉,李生秀. 施氮量对夏季玉米产量及土壤水氮动态的影响[J]. 生态学报,2005,27(1):197-204
[41] 张辰明,徐烨红,赵海娟,等. 不同氮形态对水稻苗期氮素吸收和根系生长的影响[J]. 南京农业大学学报,2011,34(3):72-76
[42] 陈效民,吴华山,孙静红. 太湖地区农田土壤中铵态氮和硝态氮的时空变异[J]. 环境科学,2006,27(6):1217-1222
[43] Hojjati S M,Templeton W C,Taylor T H. Nitrogen Fertilization in Establishing Forage Legumes1[J]. Agronomy Journal,1978,70(3):429-433
[44] Lima J D,Matta FMD,Mosquim P R. Growth attributes,xylem sap composition,and photosynthesis in common bean as affected by nitrogen and phosphorus deficiency[J]. Journal of Plant Nutrition,2000,23(7):937-947
[45] 苗艳芳,吕静霞,李生秀,等. 铵态氮肥和硝态氮肥施入时期对小麦增产的影响[J]. 水土保持学报,2014,28(4):91-96
[46] Anthoni P,Thibault C,Caroline C,et al. The nitrate transporter MtNPF6.8(MtNRT1.3) transports abscisic acid and mediates nitrate regulation of primary root growth in Medicago truncatula[J]. Plant Physiology,2014,166(4):2152-2165

Effects of Nitrogen Forms on Plant Growth,Expression of Nitrate Transporter Gene MtNRT1.3,and Nitrogen Absorption in Medicago sativa L.

不同形态氮肥对紫花苜蓿生长、硝酸盐转运蛋白基因MtNRT1.3表达及氮吸收的影响

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