Characteristics of Photosynthetic-Light Response and Photosynthetic-CO2 Response Curves in Transgenic Alfalfa MsOr Gene Tobacco

doi:10.11733/j.issn.1007-0435.2020.01.003

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (1): 20-30.DOI: 10.11733/j.issn.1007-0435.2020.01.003

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Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco

LAI Shuai-bin¹, PAN Xin-ya¹, JIAN Chun-xia¹, LI Junbao², KWAK Sang-Soo³, XU Bing-cheng^1,4, WANG Zhi^1,4

1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest Agriculture and Forestry University, Yangling, Shaanxi Province 712100, China;
2. Shaanxi Institute of Desertification Control, Yulin, Shaanxi Province 719000, China;
3. Plant Systems Engineering Research Center, Korea Research Institute of Biotechnology and Biotechnology, Daejeon 305-806, Korea;
4. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2019-10-18 Revised:2019-11-02 Online:2020-02-15 Published:2020-01-18

转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究

赖帅彬¹, 潘新雅¹, 简春霞¹, 李军保², 郭尚洙³, 徐炳成^1,4, 王智^1,4

1. 西北农林科技大学水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100;
2. 陕西省治沙研究所, 陕西榆林 719000;
3. 韩国生命工学研究院植物系统工程研究中心, 韩国大田 305-806;
4. 中国科学院水利部水土保持研究所, 陕西杨凌 712100

通讯作者: 徐炳成, 王智
作者简介:赖帅彬(1996-),男,陕西富县人,硕士研究生,主要从事水土保持植被研究,E-mail:1147812232@qq.com
基金资助:
陕西省重点研发计划（2019TSLNY03-01）；国家自然科学基金（31700335）资助

Abstract

Abstract: In order to clarify the effect of alfalfa (Medicago sativa) Orange gene expression on plant photosynthesis,the leaf light response curves and CO₂ response curves of transgenic Orange (MsOr) (TOR1 and TOR2) and wild tobacco (WT) lines were analyzed through a pot experiment. The results showed that the right angle hyperbolic correction model could better fit the tobacco light response curve and CO₂ response curve than rectangular hyperbolic model and non-rectangular hyperbolic model. Over a range of photosynthetically active radiant and CO₂ concentration,the net photosynthetic rate of TOR1 and TOR2 were always significantly higher than that of WT (P<0.05) as the increase of light intensity and CO₂ concentration;maximum net photosynthetic rate,light saturation point,CO₂ saturation point,carboxylation efficiency,dark respiration rate,and photorespiration rate of TOR1 and TOR2 were significantly higher than those of WT,while the CO₂ compensation point was significantly lower than WT (P<0.05);meanwhile,the total leaf carotenoid content of TOR1 and TOR2 were about twice that of WT. These observations indicated that the expression of MsOr gene could efficiently improve tobacco plants adaptability through enhancing the leaf carotenoid content accumulation and the photosynthesis and CO₂ utilization ability under high light intensity and high CO₂ concentration condition.

Key words: MsOr gene, Carotenoids, Photosynthesis, Maximum net photosynthetic rate, Light response curve, CO₂response curve

摘要： 为明确苜蓿（Medicago sativa）Orange基因的表达对植物光合作用的影响，以转Orange（MsOr）基因型烟草（TOR1与TOR2）和野生型烟草（WT）为研究材料，采用盆栽控制试验，比较研究了WT，TOR1和TOR2叶片光响应曲线和CO₂响应曲线，并测定其叶片类胡萝卜素含量。结果表明：直角双曲线修正模型可较好地拟合烟草光响应曲线和CO₂响应曲线；在一定光合有效辐射强度和CO₂浓度范围内，随光强和CO₂浓度增加，TOR1和TOR2的净光合速率均显著高于WT（P<0.05）；TOR1和TOR2的最大净光合速率、光饱和点、CO₂饱和点、羧化效率、暗呼吸速率和光呼吸速率显著高于WT，而CO₂补偿点显著低于WT（P<0.05）；TOR1和TOR2的叶片总类胡萝卜素含量约为WT的2倍。研究表明：苜蓿MsOr基因的表达促进了烟草植株叶片类胡萝卜素的积累，同时提高了其光能和CO₂利用能力，增强了植株对高光强、高CO₂浓度环境的适应能力。

关键词: MsOr基因, 类胡萝卜素, 光合作用, 最大净光合速率, 光响应曲线, CO₂响应曲线

CLC Number:

S311

LAI Shuai-bin, PAN Xin-ya, JIAN Chun-xia, LI Junbao, KWAK Sang-Soo, XU Bing-cheng, WANG Zhi. Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco[J]. Acta Agrestia Sinica, 2020, 28(1): 20-30.

赖帅彬, 潘新雅, 简春霞, 李军保, 郭尚洙, 徐炳成, 王智. 转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究[J]. 草地学报, 2020, 28(1): 20-30.

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References

[1] Flexas J,Bota J,Jeroni Galmés,et al. Keeping a positive carbon balance under adverse conditions:Responses of photosynthesis and respiration to water stress[J]. Physiologia Plantarum,2006,127(3):343-352
[2] Ohmiya A. Biosynthesis of plant pigments:anthocyanins,betalains and carotenoids[J]. The Plant Journal,2008,54(4):733-749
[3] Esteban R,Moran J F,Becerril J M,et al. Versatility of carotenoids:An integrated view on diversity,evolution,functional roles and environmental interactions[J]. Environmental and Experimental Botany,2015,119:63-75
[4] Wang Z,Xu W Z,Kang J Y,et al. Overexpression of alfalfa Orange gene in tobacco enhances carotenoid accumulation and tolerance to multiple abiotic stresses[J]. Plant Physiology and Biochemistry,2018,130:613-622
[5] Nisar N,Li L,Lu S,et al. Carotenoid metabolism in plants[J]. Molecular Plant,2015,8(1):68-82
[6] Lu S,Van Eck J,Zhou X,et al. The cauliflower Or gene encodes a DnaJ cysteine-rich domain-containing protein that mediates high levels of-carotene accumulation[J]. The Plant Cell,2006,18(12):3594-3605
[7] Lopez A B,Van E J,Conlin B J,et al. Effect of the cauliflower Or transgene on carotenoid accumulation and chromoplast formation in transgenic potato tubers[J]. Journal of experimental botany,2008,59(2):213-223
[8] Wang Z,Qingbo K,Duck K M,et al. Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance[J]. PLOS ONE,2015,10(5):e0126050
[9] Busch M,Seuter A,Hain R. Functional analysis of the early steps of carotenoid biosynthesis in tobacco[J]. Plant Physiology,2002,128(2):439-453
[10] Zhang J C,Tao N G,Xu Q,et al. Functional characterization of Citrus PSY gene in Hongkong kumquat (Fortunella hindsii Swingle)[J]. Plant Cell Reports,2009,28(11):1737
[11] Park S,Kim H S,Jung Y J,et al. Orange protein has a role in phytoene synthase stabilization in sweetpotato[J]. Scientific Reports,2016,6(1):33563
[12] Karppinen K,Zoratti L,Sarala M,et al. Carotenoid metabolism during bilberry (Vaccinium myrtillus L.) fruit development under different light conditions is regulated by biosynthesis and degradation[J]. BMC Plant Biology,2016,16(1):95
[13] Ido K,Ifuku K,Yamamoto Y,et al. Knockdown of the PsbP protein does not prevent assembly of the dimeric PSII core complex but impairs accumulation of photosystem II supercomplexes in tobacco[J]. Biochimica et Biophysica Acta (BBA)/Bioenergetics,2009,1787(7):873-881
[14] Ifuku K,Ido K,Sato F. Molecular functions of PsbP and PsbQ proteins in the photosystem II supercomplex[J]. Journal of Photochemistry and Photobiology B:Biology,2011,104(1-2):158-164
[15] Kang L,Kim H S,Kwon Y S,et al. IbOr regulates photosynthesis under heat stress by stabilizing IbPsbp in sweetpotato[J]. Frontiers in Plant Science,2017,8:989
[16] Goo Y M,Han E H,Jeong J C,et al. Overexpression of the sweet potato IbOr gene results in the increased accumulation of carotenoid and confers tolerance to environmental stresses in transgenic potato[J]. Comptes Rendus Biologies,2015,338(1):12-20
[17] 张丽娟,李红兵,孙振玫,等. 过表达IbOr基因甘薯增强抗盐性的生理机制[J]. 应用与环境生物学报,2017,23(1):54-59
[18] 李敏,苏国霞,熊沛枫,等. 转多抗基因新疆大叶苜蓿光合生理特征对土壤水分变化的响应[J]. 草业学报,2018,27(11):95-105
[19] 叶子飘,王怡娟,王令俐,等. 大豆叶片光呼吸对光强和CO₂浓度的响应[J]. 生态学杂志,2017,36(9):2535-2541
[20] 刘玉萍,周勇辉,吕婷,张晓宇,苏旭.青藏高原3种野豌豆光合生理特性的比较[J]. 草地学报,2017,25(1):122-129
[21] 任博,李俊,同小娟,等. 太行山南麓栓皮栎和刺槐光合作用CO₂响应模拟[J]. 应用生态学报,2018,29(1):1-10
[22] 刘超,胡正华,陈健,等. 不同CO₂浓度升高水平对水稻光合特性的影响[J]. 生态环境学报,2018,27(2):246-254
[23] Baly E C. The Kinetics of Photosynthesis[J]. Proceedings of the Royal Society of London,1935,149(868):596-596
[24] Thornley J H M. Mathematical models in plant physiology[M]. London:Academic Press,1976:86-110
[25] 叶子飘,于强. 一个光合作用光响应新模型与传统模型的比较[J]. 沈阳农业大学学报,2007,38(6):771-775
[26] 叶子飘,康华靖,段世华,等. 不同CO₂浓度下大豆叶片的光合生理生态特性[J]. 应用生态学报,2018,29(2):583-591
[27] 叶子飘. 光合作用对光和CO₂响应模型的研究进展[J]. 植物生态学报,2010,34(6):727-740
[28] Kim S H,Ahn Y O,Ahn M J,et al. Cloning and characterization of an Orange gene that increases carotenoid accumulation and salt stress tolerance in transgenic sweetpotato cultures[J]. Plant Physiology and Biochemistry,2013,70(1):445-454
[29] Cho K S. Expressing the sweet potato Orange gene in transgenic potato improves drought tolerance and marketable tuber production[J]. Comptes Rendus Biologies,2016,339(5-6):207-213
[30] 周玉霞,巨天珍,王引弟,等. 4种光响应曲线模型对3种高寒草甸植物的实用性分析[J]. 草地学报,2018,26(2):488-496
[31] 吕扬,刘廷玺,闫雪,等. 科尔沁沙丘-草甸相间地区黄柳和小叶锦鸡儿光合速率对光照强度和CO₂浓度的响应[J]. 生态学杂志,2016,35(12):3157-3164
[32] 王荣荣,夏江宝,杨吉华,等. 贝壳砂生境干旱胁迫下杠柳叶片光合光响应模型比较[J]. 植物生态学报,2013,37(2):111-121
[33] 孙小玲,许岳飞,马鲁沂,等. 植株叶片的光合色素构成对遮阴的响应[J]. 植物生态学报,2010,34(8):989-999
[34] 魏明月,云菲,刘国顺,等. 不同光环境下烟草光合特性及同化产物的积累与分配机制[J]. 应用生态学报,2017,28(1):159-168
[35] 上官周平. 冬小麦对有限水分高效利用的生理机制[J]. 应用生态学报,1999(5):567-569
[36] 钟平安,邵东,黄英金,等. 不同光环境下辣椒光合特性和瞬时水分利用效率[J]. 生态学杂志,2019,25(7):1-8
[37] Davison P A,Hunter C N,Horton P. Overexpression of β-carotene hydroxylase enhances stress tolerance in Arabidopsis[J]. Nature,2002,418(6894):203-206
[38] GÖTz T,Sandmann G,RÖMer S. Expression of a bacterial carotene hydroxylase gene (crtZ) enhances UV tolerance in tobacco[J]. Plant Molecular Biology,2002,50(1):127-140
[39] 吴爱姣,徐伟洲,郭亚力,等. 不同水肥条件下达乌里胡枝子的光合-光响应曲线特征[J]. 草地学报,2015,23(4):785-792
[40] 樊良新,刘国彬,薛萐,等. CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响[J]. 草地学报,2014,22(1):85-93
[41] Leakey A D B,Uribelarrea M,Ainsworth E A,et al. Photosynthesis,productivity and yield of maize are not affected by open-air elevation of CO₂ concentration in the absence of drought[J]. Plant physiology,2006,140(2):779-790
[42] 郭连旺,沈允钢. 高等植物光合机构避免强光破坏的保护机制[J]. 植物生理学通讯,1996,32(1):1-8

Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco

转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究

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