Molecular Cloning of PpPAP Genes and Their Expression Responses to Phosphorus Deficiency in Elephant Grass (Pennisetum purpureum)

doi:10.11733/j.issn.1007-0435.2021.03.002

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (3): 425-433.DOI: 10.11733/j.issn.1007-0435.2021.03.002

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Molecular Cloning of PpPAP Genes and Their Expression Responses to Phosphorus Deficiency in Elephant Grass (Pennisetum purpureum)

HUANG Rui¹, LUO Jia-jia¹, WU Yuan-hang², LIU Pan-dao¹, LIU Guo-dao¹

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China;
2. College of Tropical Crops Hainan University, Haikou, Hainan Province 571101, China

Received:2020-09-27 Revised:2020-11-09 Online:2021-03-15 Published:2021-04-02

狼尾草属象草PpPAPs基因的克隆及响应低磷胁迫的表达模式分析

黄睿¹, 罗佳佳¹, 吴远航², 刘攀道¹, 刘国道¹

1. 中国热带农业科学院热带作物品种资源研究所, 农业部华南作物基因资源与种质创制重点实验室, 海南海口 571101;
2. 海南大学热带作物学院, 海南海口 570228

通讯作者: 刘攀道, 刘国道
作者简介:黄睿(1989-),男,研究实习员,硕士,主要从事草业科学方面研究,E-mail:bluesing@126.com
基金资助:
海南省自然科学基金项目（No.319QN309）；国家牧草产业技术体系岗位科学家项目（No.CARS-34）；中国科协青年人才托举工程第五届项目（No.2019QNRC001）；滇桂黔石漠化地区特色作物产业发展关键技术集成示范项目（No.SMH2019-2021）资助

Abstract

Abstract: To insight into mechanisms of elephant grass adapting to low phosphorus (P) stress,the effects of different P sources on elephant grass growth were investigated by hydroponic experiments. Three PpPAP genes related to ATP utilization were cloned from elephant grass by homologous cloning and rapid amplification of cDNA ends (RACE) technology. Results showed that dry weight and total P content at the application of adenosine triphosphate (+ATP) treatment were significantly higher than those at low P (-P) treatment,suggesting that elephant grass has a strong ability to utilize ATP. QRT-PCR analysis showed that after 10 days of growth,the expression of these three PpPAPs associated with ATP utilization in the roots was 170%~403% greater than that of in +ATP treated plants compared to those grown under normal P (+P) treatment. Furthermore,+ATP treatment led to significant increases in the activities of root intracellular and secreted acid phosphatase (ACP),compared to +P treatment. Taken together,the results herein suggest that the expression of PpPAP genes in roots of elephant grass were up-regulated by P deficiency,which may promote the synthesis and secretion of ACP. These adaptive responses of elephant grass may contribute to the utilization of ATP under low P stress.

Key words: Purple acid phosphatase, Phosphorus deficiency, Organic phosphorus, Elephant grass

摘要： 为解析象草(Pennisetum purpureum)适应低磷胁迫机理，本研究通过水培试验，分析了不同磷源处理对象草生长的影响，并利用同源克隆和cDNA末端快速扩增(rapid amplification of cDNA ends，RACE)技术，从象草中克隆了3个可能参与ATP利用的PAP基因。结果表明，在腺嘌呤核苷三磷酸处理(+ATP)下，象草的干重和全磷含量显著高于低磷处理(-P)，表明象草对ATP具有较强的利用能力。荧光定量PCR分析表明，与正常供磷处理(+P)相比，+ATP处理10 d使3个可能参与ATP利用的PAP基因在象草根系中的表达量提高170%~403%，并伴随着根系胞内和分泌的酸性磷酸酶(acid phosphatase,ACP)活性显著增加。综上所述，在低磷胁迫条件下，象草根系中PpPAPs基因上调表达，可能促进了根系ACP的合成与分泌，这有助于象草对ATP的活化利用。

关键词: 紫色酸性磷酸酶, 低磷胁迫, 有机磷, 象草

CLC Number:

S543

HUANG Rui, LUO Jia-jia, WU Yuan-hang, LIU Pan-dao, LIU Guo-dao. Molecular Cloning of PpPAP Genes and Their Expression Responses to Phosphorus Deficiency in Elephant Grass (Pennisetum purpureum)[J]. Acta Agrestia Sinica, 2021, 29(3): 425-433.

黄睿, 罗佳佳, 吴远航, 刘攀道, 刘国道. 狼尾草属象草PpPAPs基因的克隆及响应低磷胁迫的表达模式分析[J]. 草地学报, 2021, 29(3): 425-433.

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Molecular Cloning of PpPAP Genes and Their Expression Responses to Phosphorus Deficiency in Elephant Grass (Pennisetum purpureum)

狼尾草属象草PpPAPs基因的克隆及响应低磷胁迫的表达模式分析

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