Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (10): 2558-2571.DOI: 10.11733/j.issn.1007-0435.2022.10.005

Previous Articles    

Integrated Physiological and Transcriptomic Analyses Revealed Candidate Genes for Salt Resistance in Arundo donax

SUN Yuan-chang, LUO Lin, LIN Hui, LIN Dong-mei, LIN Zhan-xi   

  1. National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China
  • Received:2022-06-01 Revised:2022-07-18 Published:2022-11-05

综合生理学和转录组学揭示芦竹耐盐的候选基因

孙源长, 罗琳, 林辉, 林冬梅, 林占熺   

  1. 福建农林大学国家菌草工程技术研究中心, 福建 福州 350002
  • 通讯作者: 林冬梅,E-mail:lzxjuncao@163.com;林占熺,E-mail:lindm_juncao@163.com
  • 作者简介:孙源长(1997-),男,河南信阳人,硕士研究生,主要从事草类植物抗逆研究,E-mail:sunyc0000@163.com
  • 基金资助:
    福建省重大专项“菌草种质创新及其产业化利用关键技术研究与应用”(2021 NZ029009);学科交叉融合推动菌草科学及产业高质量发展(XKJC-712021030)共同资助

Abstract: In order to enrich the salt tolerance gene resources of Arundo donax and provide a theoretical basis for its salt tolerance breeding. In this study,Arundo donax with consistent growth were treated with different concentrations of NaCl salt solution for 36 h,and the physiological indicators related to stress resistance were determined. Concurrently,transcriptome sequencing and bioinformatics analyses were carried out. The results showed that the salt stress had obvious effects on the content of proline and soluble sugar of Arundo donax,while other physiological indicators did not change significantly,which indicated that it may not be very sensitive to the salt environment and has certain tolerance. The differentially expressed genes between samples were screened,and a total of 166 core differentially expressed genes were screened in leaves. Some genes with larger fold differences may play an important role in the process of salt response. The enrichment results of GO and KEGG indicated that Arundo donax mainly responded to salt stress through nitrate-related,nitric oxide-related,reactive nitrogen-related,reactive oxygen-related,flavonoid biosynthesis,and other related pathways. The co-expression network of differentially expressed genes was constructed by WGCNA,and hub genes associated with changes in proline content were screened. In conclusion,this study provides a basis for the excavation of salt tolerance genes of Arundo donax and a theoretical foundation for future molecular breeding.

Key words: Arundo donax, Salt stress, Physiological, Transcriptome, WGCNA

摘要: 为了丰富芦竹(Arundo donax)的耐盐基因资源并为耐盐育种提供理论基础。本研究对长势一致的芦竹分别用不同浓度的NaCl盐溶液处理36 h,测定与抗逆相关的生理指标,同时进行转录组测序和生物信息学分析。结果表明:本次盐胁迫对芦竹的脯氨酸和可溶性糖含量影响较为明显,而其他生理指标变化不显著,说明其对盐环境可能并不十分敏感,具有一定的耐受性。对样本间的差异表达基因进行筛选,共筛选到166个核心差异表达基因,而一些差异倍数较大的基因可能在盐响应的过程中发挥着重要作用。GO与KEGG的富集结果表明芦竹通过硝酸盐、一氧化氮、活性氮、活性氧、类黄酮生物合成等相关途径来响应盐胁迫。利用WGCNA构建差异表达基因的共表达网络,筛选出与脯氨酸含量变化相关的hub基因。总之,本研究为芦竹耐盐基因的挖掘提供依据,也为今后的分子育种奠定了理论基础。

关键词: 芦竹, 盐胁迫, 生理, 转录组, WGCNA

CLC Number: 

京ICP备10039388号

Acta Agrestia Sinica

Supported by:Beijing Magtech