Effects of Mixed-Sowing of Artemisia wellbyi and Perennial Grasses on Soil Enzyme Activities and Microbial Biomass of Desertified Grassland in Tibet

doi:10.11733/j.issn.1007-0435.2022.12.008

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (12): 3233-3240.DOI: 10.11733/j.issn.1007-0435.2022.12.008

Effects of Mixed-Sowing of Artemisia wellbyi and Perennial Grasses on Soil Enzyme Activities and Microbial Biomass of Desertified Grassland in Tibet

DONG Kai¹, LIU Huan¹, LIU Yun-fei², WANG Jing-long², REN Zengwangdui², WANG Qi-yu¹, LUO Jian-min¹, YAO Rui-rui¹, ZHANG Xiao¹

1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu Province 730070, China;
2. Institute of Pratacultural Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa, Tibet 850000, China

Received:2022-05-19 Revised:2022-08-28 Published:2023-01-04

藏沙蒿与多年生禾草混播对西藏沙化草地土壤酶活性和微生物生物量的影响

董凯¹, 刘欢¹, 刘云飞², 王敬龙², 仁增旺堆², 王绮玉¹, 罗建民¹, 姚瑞瑞¹, 张晓¹

1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室, 甘肃兰州 730070;
2. 西藏自治区农牧科学院草业科学研究所, 西藏拉萨 850000

通讯作者: 刘欢,E-mail:liuhuan@gsau.edu.cn;刘云飞,E-mail:306027640@qq.com
作者简介:董凯(1998-),男,汉族,甘肃靖远人,硕士研究生,主要从事草地生物多样性研究,E-mail:344884621@qq.com
基金资助:
西藏自治区重点研发及转化计划-西藏藏沙蒿和大籽蒿资源评价与应用技术研究(XZ201902 NB01)资助

Abstract

Abstract: The characteristics of soil enzyme activity and microbial biomass content of desertified grassland mix-sown with Artemisia wellbyi and perennial grasses for 3 years were analyzed,and the mixing ratio with the best remediation effect was screened out. In this study,colorimetric and chloroform fumigation methods were used to determine soil enzyme activity and microbial biomass under different treatments. Artemisia wellbyi,Elymus nutans and Agropyron trachycaulhum were treated with different mixed sowing ratios,T₁ (4:1:2),T₂ (4:2:1),T₃ (5:1:1). At the same time,unrepaired sandy grassland was used as control (CK) and compared with natural grassland (NG). The results showed that:Compared with CK,the three proportions of mixed sowing treatments could improve soil physical and chemical properties,increase soil enzyme activities and microbial biomass，carbon,nitrogen and phosphorus contents,and the effect of T₃ treatment was the best. Correlation analysis showed that soil enzyme activities were positively correlated with organic matter,available potassium,and total nitrogen;soil microbial biomass was positively correlated with available phosphorus,alkalyzable nitrogen and water content. Therefore,properly increasing the proportion of shrubs in the mixture of shrub and grass is more conducive to the restoration of soil nutrients and biological characteristics.

Key words: Tibet, Ecological restoration, Enzyme activity, Soil microbial biomass

摘要： 为分析藏沙蒿(Artemisia wellbyi)与多年生禾草混播3年的土壤酶活性和微生物生物量的含量特征，筛选出修复效果最好的混播比例，本研究采用比色和氯仿熏蒸的方法分别测定不同处理下的土壤酶活性和微生物生物量，以藏沙蒿、垂穗披碱草(Elymus nutans)和细茎冰草(Agropyron trachycaulhum)不同比例混播为处理，分别为T₁(4∶1∶2)，T₂(4∶2∶1)和T₃(5∶1∶1)，同时以未修复沙化草地为对照(CK)，并与天然草地(NG)作比较。结果表明：与CK相比，3个比例混播处理均能改善土壤理化性质、提高土壤酶活性和微生物生物量碳氮磷含量，以T₃处理的效果最好。相关性分析表明，土壤酶活性与有机质、速效钾和全氮呈正相关；土壤微生物生物量与有效磷、碱解氮和含水量呈正相关。因此，灌草混播时,适当增加灌木的占比有利于土壤养分及生物学特性的恢复。

关键词: 西藏, 生态修复, 酶活性, 土壤微生物量

CLC Number:

S154.3

DONG Kai, LIU Huan, LIU Yun-fei, WANG Jing-long, REN Zengwangdui, WANG Qi-yu, LUO Jian-min, YAO Rui-rui, ZHANG Xiao. Effects of Mixed-Sowing of Artemisia wellbyi and Perennial Grasses on Soil Enzyme Activities and Microbial Biomass of Desertified Grassland in Tibet[J]. Acta Agrestia Sinica, 2022, 30(12): 3233-3240.

董凯, 刘欢, 刘云飞, 王敬龙, 仁增旺堆, 王绮玉, 罗建民, 姚瑞瑞, 张晓. 藏沙蒿与多年生禾草混播对西藏沙化草地土壤酶活性和微生物生物量的影响[J]. 草地学报, 2022, 30(12): 3233-3240.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.12.008

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I12/3233

References

[1] 安宝晟,姚檀栋,郭燕红,等. 拉萨河流域典型区域保护、修复、治理技术示范体系[J]. 科学通报,2021,66(22):2775-2784
[2] 朱灵,李易,杨婉秋,等. 沙化对高寒草地土壤碳、氮、酶活性及细菌多样性的影响[J]. 水土保持学报,2021,35(3):350-358
[3] 张潇喜,曾凯,蔡义民,等. 青藏高原东部乡土植物对高寒沙化草地土壤性质的影响[J]. 草业科学,2018,35(4):749-759
[4] 吴鹏. 浅析生态修复的法律定义[J]. 环境与可持续发展,2011,36(3):63-66
[5] 王敬龙,史睿智,仁增旺堆,等. 西藏草地藏沙蒿资源分布调查[J]. 西藏农业科技,2018,40(1):40-42
[6] 常学向,陈怀顺,李志刚,等. 西藏雅鲁藏布江流域典型沙漠区生态修复植物群落物种多样性[J]. 中国沙漠,2021,41(6):187-194
[7] 龙建廷,高献磊,包赛很那,等. 垂穗披碱草抗逆性研究进展[J]. 草学,2022(3):1-8
[8] 王玲玲,库努都孜阿依·吐鲁洪,孟广飞,等. 土壤水分和植株密度互作对垂穗披碱草地下营养繁殖及生物量的影响[J]. 草业学报,2021,30(11):52-61
[9] 王柳英,颜红波,周青平,等. 高寒山区细茎冰草生物学特性及经济性状的观察[J]. 青海畜牧兽医杂志,2004(3):12-13
[10] 纪童,蒋齐,王占军,等. 7种禾本科牧草抗旱性研究与评价[J]. 草业学报,2022,31(7):144-156
[11] 史长光. 川西北退化、沙化草原植被恢复效果研究[D]. 成都:四川师范大学,2010:14-40
[12] 胡金娇,周青平,吕一河,等. 青藏高原东缘半湿润沙地典型生态恢复模式的效果比较研究[J]. 生态学报,2020,40(20):7410-7418
[13] 杨满元,杨宁,欧阳美娟,等. 衡阳紫色土丘陵坡地5种生态恢复模式的效果比较[J]. 草地学报,2020,28(1):177-183
[14] 杨富玲,石杨,李斌,等. 植物根系分泌物在污染及沙化土壤修复中的应用现状与前景[J]. 应用生态学报,2021,32(7):2623-2632
[15] 朱蒙恩,沙利云,周明涛,等. 西藏高寒地区不同边坡生态修复类型土壤的肥力变化[J]. 水土保持通报,2021,41(4):158-165
[16] LI X,LEI S,LIU F,et al. Analysis of plant and soil restoration process and degree of refuse dumps in Open-Pit coal mining areas[J]. Int J Environ Res Public Health,2020,17(6):1975
[17] FENG X,QU J,FAN Q,et al. Characteristics of desertification and short-term effectiveness of differing treatments on shifting sand dune stabilization in an alpine rangeland[J]. Int J Environ Res Public Health,2019,16(24):4968
[18] 李巧玲,肖忠,任明波,等. 间作不同作物对栀子根际土壤微生态的影响[J]. 微生物学通报,2021,48(10):3588-3602
[19] 谢东,李丝雨,何森,等. 重金属污染土壤修复植物根际微生态的研究进展[J]. 江西理工大学学报,2019,40(5):64-71
[20] 岳雪娇,黄沛,李永华,等. 中国干旱半干旱区土壤微生物分布特征及其影响因素[J]. 世界林业研究,2022,35(4):64-69
[21] 张建军,党翼,赵刚,等. 留膜留茬免耕栽培对旱作玉米田土壤养分、微生物数量及酶活性的影响[J]. 草业学报,2020,29(2):123-133
[22] 周际海,郜茹茹,魏倩,等. 旱地红壤不同土地利用方式对土壤酶活性及微生物多样性的影响差异[J]. 水土保持学报,2020,34(1):327-332
[23] GONG J,HOU W,LIU J,et al. Effects of different land use types and soil depths on soil mineral elements,soil enzyme activity,and fungal community in Karst Area of Southwest China[J]. Int J Environ Res Public Health.2022,19(5):3120
[24] 李静,蔚晓燕,唐明. 黄土高原纸坊沟流域不同植物对土壤微生物生物量和土壤酶活性的影响[J]. 西北植物学报,2013,33(2):387-393
[25] 闫瑞瑞,卫智军,乌仁其其格,等. 微生物肥料对呼伦贝尔打孔羊草草甸草原土壤微生物及酶活性的影响研究[J]. 生态环境学报,2017,26(4):597-604
[26] 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社,2006:25-103
[27] 吴金水,林启美,黄巧云,等. 土壤微生物生物量测定方法及其应用[M]. 北京:气象出版社,2006:54-95
[28] 关松荫. 土壤酶及其研究法[M]. 北京:农业出版社,1986:274-338
[29] LIN Y,YE Y,LIU S,et al. Effect mechanism of land consolidation on soil bacterial community:a case study in eastern China[J]. International journal of environmental research and public health,2022,19(2):845
[30] 全国土壤普查办公室. 中国土壤[M]. 北京:农业出版社,1998:843-984
[31] 张世军. 生态修复工程实施后对土壤理化性质改善效益初探[J]. 干旱环境监测,2010,24(1):31-33
[32] 赵啸龙,谢玉鸿,马旭君,等. 科尔沁沙质草地不同恢复年限草本层群落结构及其与土壤理化性质的关系[J]. 中国沙漠,2022,42(2):134-141
[33] 王凯博,时伟宇,上官周平. 黄土丘陵区天然和人工植被类型对土壤理化性质的影响[J]. 农业工程学报,2012,28(15):80-86
[34] ZHANG X,ZHANG F,WANG D,et al. Effects of vegetation,terrain and soil layer depth on eight soil chemical properties and soil fertility based on hybrid methods at urban forest scale in a typical loess hilly region of China[J]. PloS one,2018,13(10):e0205661
[35] ZHAO Q,TANG J,LI Z,et al. The influence of soil physico-Chemical properties and enzyme activities on soil quality of saline-alkali agroecosystems in western Jilin Province,China[J]. Sustainability,2018,10(5):1529
[36] ZHU S C,ZHENG H X,LIU W S,et al. Plant-Soil Feedbacks for the Restoration of Degraded Mine Lands:A Review[J]. Frontiers in Microbiology,2021,12:751794
[37] 刘小龙,胡健,周青平,等. 若尔盖高原典型草地灌丛化对植被特征和土壤养分的影响[J]. 草地学报,2022,30(4):901-908
[38] SHANG L,WAN L,ZHOU X,et al. Effects of organic fertilizer on soil nutrient status,enzyme activity,and bacterial community diversity in Leymus chinensis steppe in Inner Mongolia,China[J]. PloS one,2020,15(10):e0240559
[39] 巩杰,陈利顶,傅伯杰,等. 黄土丘陵区小流域植被恢复的土壤养分效应研究[J]. 水土保持学报,2005(1):93-96
[40] YANG Y,LI M,WU J,et al. Impact of Combining Long-Term subsoiling and organic fertilizer on soil microbial biomass carbon and nitrogen,soil enzyme activity,and water use of winter wheat[J]. Frontiers in Plant Science,2021(12):788651
[41] XIAO F,LI Y,LI G,et al. High throughput sequencing-based analysis of the soil bacterial community structure and functions of Tamarix shrubs in the lower reaches of the Tarim River[J]. PeerJ,2021(9):e12105
[42] 陈小花,陈宗铸,雷金睿,等. 清澜港红树林湿地土壤酶活性与理化性质的关系[J]. 林业科学研究,2022,35(2):171-179
[43] 于铁峰,郝凤,张永亮,等. 沙地豆禾混播草地土壤酶与土壤养分对混播比例的响应[J]. 草地学报,2021,29(6):1217-1223
[44] 吴宇伦,赵雪淞,宋王芳,等. 花生和玉米种植模式对科尔沁沙地土壤养分和生物特性的影响[J]. 安徽农业科学,2022,50(5):48-54
[45] 傅民杰,王传宽,王颖,等. 四种温带森林土壤氮矿化与硝化时空格局[J]. 生态学报,2009,29(7):3747-3758
[46] 靳振江,贾远航,潘复静,等. 会仙岩溶湿地4种覆被下土壤酶活性和微生物生物量[J]. 生态学报,2021,41(21):8464-8471
[47] YANG N,ZOU D,YANG M,et al. Variations in soil microbial biomass carbon and soil dissolved organic carbon in the Re-Vegetation of Hilly Slopes with Purple Soil[J]. PLoS One,2016,11(12):e0166536

Effects of Mixed-Sowing of Artemisia wellbyi and Perennial Grasses on Soil Enzyme Activities and Microbial Biomass of Desertified Grassland in Tibet

藏沙蒿与多年生禾草混播对西藏沙化草地土壤酶活性和微生物生物量的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Ya-nan, LI Shao-yu, SHI Shi-bin, WANG Zhan-hai, ZHANG Bin, CHEN Heng, ZHAO Meng-li. Study on Soil Enzyme Stoichiometric Characteristics of Different Grazing Intensities in Desert Steppe [J]. Acta Agrestia Sinica, 2022, 30(9): 2239-2248.
[2]	SU Wen-xin, XU Ling-xin, JIANG Wan-tong, LIU Yu-le, WANG Fei, LYU Ya-ru, YAN Jun-xin. Effects of Different Exogenous Substances on Seed Germination and Seedling Growth and Physiology of Perilla frutescens under Saline-alkali Stress [J]. Acta Agrestia Sinica, 2022, 30(9): 2415-2422.
[3]	FEI Xuan, SUO Cai-xu, XIANG Shuang, SUN Shu-cun. Responses of Plant Community Structure and Functional Group Characteristics to Long-term Seasonal Grazing in an Alpine Meadow on Eastern Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2022, 30(8): 1954-1963.
[4]	LI Ya-nan, LI Shao-yu, SUN Yu, HU Xue-feng, HE Qi-shen, ZHANG Bin, ZHAO Meng-li. Responses of Soil Enzyme Activities and Stoichiometry Characteristics of Desert Steep to Different Stocking Rates [J]. Acta Agrestia Sinica, 2022, 30(8): 2019-2026.
[5]	MA Kai-kai, XU Chang-lin, LI Ying, WEI Kong-tao, BAI Mei-mei, LIU Yuan-yuan, YU Xiao-jun. Effects of Different Rest Grazing Periods on Soil Seed Bank in Cold Season Pasture of Alpine Meadow [J]. Acta Agrestia Sinica, 2022, 30(8): 2100-2107.
[6]	JI Zhen-xia, PEI Ting-ting, CHEN Ying, HOU Qing-qing, XIE Bao-peng, WU Hua-wu. Spatial-Temporal Variation and Driving Factors of Grassland NDVI in the Qinghai-Tibet Plateau from 2001 to 2020 [J]. Acta Agrestia Sinica, 2022, 30(7): 1873-1881.
[7]	SHAO Zi-tong, QIN Yu. Advance in Studying the Influence of Plateau Pikas Disturbance on Carbon Cycle of Alpine Grasslands [J]. Acta Agrestia Sinica, 2022, 30(5): 1086-1094.
[8]	BASANG Can-mujue, WEN Zhong-ming, LIU Yang-yang, ZHOU Rong-lei, REN Han-yu, LIN Zi-qi, ZHANG Zhi-xin, WANG Yi-bo, ZHANG Ge-yu. Analysis of the Spatial-Temporal Evolution Patterns of Grassland Net Primary Productivity and Its Driving Mechanisms in the Tibet [J]. Acta Agrestia Sinica, 2022, 30(4): 778-789.
[9]	FENG Yue, BAI Wei, ZHANG Jing-ran, FANG Jia-chen, ZENG Hong-li. Evaluation of Physiological Response and Adaptability of Plants in Alpine Swamp Meadows in the Headwaters Region of the Yangtze River to Warming [J]. Acta Agrestia Sinica, 2022, 30(4): 790-800.
[10]	LIU Xiao-long, HU Jian, ZHOU Qing-ping, CAO Quan-heng, SUN Mei-ling, CHEN Xue-ling, YANG Li-xue. Effects of Typical Shrub-encroached Grassland on Vegetation Characteristics and Soil Nutrients in the Zoige Plateau [J]. Acta Agrestia Sinica, 2022, 30(4): 901-908.
[11]	LU Gang, HUANG Hai-xia, ZHOU Xiao-lei, CAO Xue-ping, ZHAO An. Characteristics of Soil Organic Carbon and Changes of Enzyme Activities in Burned Area of Spruce-Fir Forests in Diebu Forest Region [J]. Acta Agrestia Sinica, 2022, 30(4): 943-949.
[12]	CAO Ya-nan, SUN Ming-xiang, CHEN Meng-ran, HE Yong-tao, SONG Hong-li. Effects of Precipitation on the Vegetation Coverage in the Northern Tibetan Plateau from 2000 to 2016 [J]. Acta Agrestia Sinica, 2022, 30(3): 721-730.
[13]	TONG Yong-shang, ZHANG Chun-ping, YU Yang, CAO Quan, YANG Zeng-zeng, HUO Li-an, YANG Xue, ZHANG Xiao-fang, LI Cai-di, DONG Quan-min. Quasi-Stable State Theory and Maintenance Technology in Perennial Mixed-Sowing Artificial Grassland [J]. Acta Agrestia Sinica, 2022, 30(11): 2845-2855.
[14]	QU Song-lin, WU Yi-fan, LIU Zhong-kuan, WANG Guo-liang, CHEN Yan-jing, RONG Yu-ping. Research Progress for Effects of Arbuscular Mycorrhizal Fungi on Growth and Development of Alfalfa [J]. Acta Agrestia Sinica, 2022, 30(10): 2529-2534.
[15]	TIAN Yu-li, WU Xiao-ping, CHEN Xin-Fo, GUO Ya-ru, LI Ze-lin, NI Xin-hua, ZHANG Jia-tao, LI Hui-ke. Effect of Diversity Mulching Model on Soil Enzyme Activities in the Loess Plateau Orchard [J]. Acta Agrestia Sinica, 2022, 30(10): 2581-2589.