Effects of Exogenous GABA on Seed Germination and Physiological Characteristics of Elymus nutans under NaCl Stress

doi:10.11733/j.issn.1007-0435.2022.02.018

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (2): 403-411.DOI: 10.11733/j.issn.1007-0435.2022.02.018

Effects of Exogenous GABA on Seed Germination and Physiological Characteristics of Elymus nutans under NaCl Stress

SONG Jian-chao, YANG Hang, JING Yuan-yuan, CHEN Yan-zhu, YU Xiao-jun

College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Grassland Engineering Labroatory of Gansu Province, Sion-U.S. Centers for Grazing Land Ecosystem Sustainability, Pratacultural Engineering Laboratory of Gansu Province, Lanzhou, Gansu Province 730070, China

Received:2021-09-18 Revised:2021-10-20 Published:2022-03-10

外源GABA对NaCl胁迫下垂穗披碱草种子萌发及幼苗生理特性的影响

宋建超, 杨航, 景媛媛, 陈彦珠, 鱼小军

甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 鱼小军,E-mail:yuxj@gsau.edu.cn
作者简介:宋建超(1996-),男,汉族,甘肃张掖人,硕士研究生,主要从事饲草栽培与育种研究,E-mail:1339801334@qq.com
基金资助:
甘肃省草原生态修复治理科技支撑项目;青海省重点研发与转化计划(2019-HZ-815)资助

Abstract

Abstract: In this study,Elymus nutans was taken as the research object to explore the effects of exogenous γ-Aminobutyric acid (GABA) on seed germination and seedling physiological characteristics of Elymus nutans under NaCl stress,which was of great significance for the development and utilization of saline alkali land and the ecological restoration of saline grassland. The results showed that 100 mmol·L^-1 NaCl salt stress inhibited the seed germination and seedling growth of Elymus nutans. Under salt stress,the addition of 0.05~2.00 mmol·L^-1GABA promoted the seed germination and seedling growth of Elymus nutans. The average increase of germination potential and germination ratio under different concentrations of GABA was 109.77% and 19.48%,respectively,compared with salt stress. Under salt stress,the addition of 0.05~2.00 mmol·L^-1GABA treatment significantly increased the soluble sugar,soluble protein,proline content and antioxidant enzyme activity of Elymus nutans seedlings,and reduced malondialdehyde (MDA) content,thereby effectively reduced the oxidative damage caused by NaCl stress and improved the salt tolerance during the germination of Elymus nutans seeds. The comprehensive evaluation showed that the addition of 0.5 mmol·L^-1 exogenous GABA had the best effect on promoting seed germination and seedling growth of Elymus nutans under NaCl stress,and improved the salt resistance of Elymus nutans at the germination stage.

Key words: Elymus nutans, γ-Aminobutyric acid, NaCl stress, Seed germination, Physiological characteristics

摘要： 本研究以垂穗披碱草(Elymus nutans)为研究对象，探究了外源γ-氨基丁酸(γ-aminobutyric acid，GABA)对NaCl胁迫下垂穗披碱草种子的萌发和幼苗生理特性的影响，对盐碱地的开发利用和盐渍化草地的生态修复具有重要意义。研究结果表明，100 mmol·L^-1NaCl盐胁迫抑制了垂穗披碱草种子的萌发和幼苗生长，外源添加0.05~2 mmol·L^-1GABA可促进NaCl胁迫下垂穗披碱草种子的萌发和幼苗生长。与NaCl胁迫处理相比，垂穗披碱草种子发芽势和发芽率在不同浓度GABA处理下的平均增幅分别为109.77%和19.48%。外源添加0.05~2 mmol·L^-1GABA处理能够显著提高NaCl胁迫下垂穗披碱草幼苗可溶性糖、可溶性蛋白、脯氨酸含量和抗氧化酶活性，降低丙二醛含量，从而有效降低NaCl胁迫导致的氧化损伤，提高垂穗披碱草种子萌发过程的耐盐性。采用隶属函数综合评价得出：添加0.5 mmol·L^-1外源GABA对促进NaCl胁迫下垂穗披碱草种子萌发和幼苗生长效果最佳，改善了垂穗披碱草种子萌发阶段的抗盐性。

关键词: 垂穗披碱草, γ-氨基丁酸, NaCl胁迫, 种子萌发, 生理特性

CLC Number:

Q945.78

SONG Jian-chao, YANG Hang, JING Yuan-yuan, CHEN Yan-zhu, YU Xiao-jun. Effects of Exogenous GABA on Seed Germination and Physiological Characteristics of Elymus nutans under NaCl Stress[J]. Acta Agrestia Sinica, 2022, 30(2): 403-411.

宋建超, 杨航, 景媛媛, 陈彦珠, 鱼小军. 外源GABA对NaCl胁迫下垂穗披碱草种子萌发及幼苗生理特性的影响[J]. 草地学报, 2022, 30(2): 403-411.

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Effects of Exogenous GABA on Seed Germination and Physiological Characteristics of Elymus nutans under NaCl Stress

外源GABA对NaCl胁迫下垂穗披碱草种子萌发及幼苗生理特性的影响

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