Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover

doi:10.11733/j.issn.1007-0435.2022.04.013

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (4): 879-888.DOI: 10.11733/j.issn.1007-0435.2022.04.013

Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover

LI Hui-ping, GAN Ya-nan, HAN Qing-qing, YAO Dan, CHEN Jia, ZHANG Ming-xu, HE Ao-lei, ZHANG Jin-lin, ZHAO Qi

Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Center for Grassland Microbiome, State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730020, China

Received:2021-09-29 Revised:2021-11-05 Published:2022-04-25

祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应

李慧萍, 甘雅楠, 韩庆庆, 姚丹, 陈佳, 张明旭, 何傲蕾, 张金林, 赵祺

兰州大学农业农村部草牧业创新重点实验室, 兰州大学草地微生物研究中心, 兰州大学草地农业生态系统国家重点实验室, 兰州大学草地农业科技学院, 甘肃兰州 730020

通讯作者: 张金林,E-mail:jlzhang@lzu.edu.cn;赵祺,E-mail:qzhao@lzu.edu.cn
作者简介:李慧萍(1994-),女,汉族,甘肃古浪人,博士研究生,主要从事极端环境微生物多样性和根际促生细菌的研究,E-mail:lihp19@lzu.edu.cn
基金资助:
国家自然科学基金项目(31801944);中央高校基本科研业务费专项资金(lzujbky-2021-ct16);国家重点研发计划(2019YFC0507703)资助

Abstract

Abstract: In order to obtain excellent phosphate solubilizing bacteria (PSB) strains that could promote the growth of white clover (Trifolium repens L.),soil collected from spruce forests (Picea crassifolia) in the Qilian Mountains were served as our research object in this study.We used phosphate-dissolving qualitative and quantitative methods,phylogenetic analysis and plant inoculation experiments to investigate the phosphate solubilizing ability,taxonomic status of PSB strains and evaluate their inoculation effects on the growth of white clover.The results showed that the ratio of the diameters of the halo to the colony on NBRIP plates of 12 PSB strains were ranging from 1.44 to 2.40.These strains were further identified and classified as Pseudomonas sp..On the basis of above data,the soluble phosphorus content of 5 selected PSB strains was ranging from 387.41 μ g·mL^-1 to 479.87 μ g·mL^-1 in the NBRIP liquid medium after 96 hours of incubation.The content of soluble phosphorus in the fermentation broth of strain LT-4 was the highest in all strains.In addition,we observed that the application of strain LT-4 significantly improved the growth of white clover compared to DH5α and water treatment,respectively,especially,shoot and root dry weight significantly increased by 87.01% and 74.83%.These results could lay a foundation for further exploring the regulatory mechanism and high-efficiency biofertilizer for forage cultivation.

Key words: Phosphate-solubilizing bacteria, Pseudomonas, White clover, Growth-promotion

摘要： 为获得具有促进牧草白三叶(Trifolium repens L.)生长能力的优良溶磷细菌(Phosphate solubilizing bacteria,PSB)资源,本研究以祁连山青海云杉林(Picea crassifolia)根际土壤细菌为研究对象,采用平板定性与钼锑抗比色定量分析、系统进化分析和植物盆栽接种试验,探究了上述根际土壤所分离菌株的溶磷能力、分类地位和对白三叶生长的影响。结果表明:筛选得到的12株PSB菌株在难溶性无机磷(National botanical research institute phosphorus,NBRIP)平板上的溶磷圈直径/菌落直径的比值范围为1.44~2.40。12株PSB菌株均归属于假单胞菌属(Pseudomonas sp.)。在上述基础上,选取的5株菌株在NBRIP液体培养基培养96 h后,发酵液的可溶性磷含量范围为387.41~479.87 μg·mL^-1,其中菌株LT-4发酵液的可溶性磷含量最高。植物接种试验结果表明,与空白对照相比,接种菌株LT-4能显著提高白三叶的生物量,植株地上部和根干重分别显著增加87.01%和74.83%。本研究为进一步探究溶磷细菌的促生机理以及开发应用于栽培牧草的微生物菌剂或菌肥奠定了一定的前期工作基础。

关键词: 溶磷细菌, 假单胞菌属, 白三叶, 促生效应

CLC Number:

Q93-Q939.3

LI Hui-ping, GAN Ya-nan, HAN Qing-qing, YAO Dan, CHEN Jia, ZHANG Ming-xu, HE Ao-lei, ZHANG Jin-lin, ZHAO Qi. Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover[J]. Acta Agrestia Sinica, 2022, 30(4): 879-888.

李慧萍, 甘雅楠, 韩庆庆, 姚丹, 陈佳, 张明旭, 何傲蕾, 张金林, 赵祺. 祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应[J]. 草地学报, 2022, 30(4): 879-888.

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Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover

祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应

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