Allelopathic Effect and Physiological Mechanism of Extracts from different Parts of Euphorbia jolkinii on Growth of Loium Perenne Seedlings

doi:10.11733/j.issn.1007-0435.2023.10.034

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (10): 3212-3219.DOI: 10.11733/j.issn.1007-0435.2023.10.034

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Allelopathic Effect and Physiological Mechanism of Extracts from different Parts of Euphorbia jolkinii on Growth of Loium Perenne Seedlings

LUO Qin¹, MA Zu-yan¹, NIU Qiong-mei¹, GUI Bao-lin², XIE Yong³, LIU Yang⁴, LIU Jia⁵, SHAN Gui-lian¹, CHU Xiao-hui¹

1. Department of Grassland Science, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
2. Xundian county Agriculture and rural Bureau soil fertilizer workstation, Xundian, Yunnan Province 655200, China;
3. Kunming Municipal Animal Disease Prevention and Control Center, Kunming, Yunnan Province 650223, China;
4. Forestry and Grassland Bureau of Yunnan Province, Kunming, Yunnan Province 650224, China;
5. Forestry and Grassland Bureau of Shangri La, Diqing, Yunnan Province 674499, China

Received:2023-04-26 Revised:2023-06-06 Online:2023-10-15 Published:2023-11-02

大狼毒不同部位浸提液对多年生黑麦草幼苗生长的化感效应及生理机制

罗钦¹, 马祖艳¹, 牛琼梅¹, 桂宝林², 谢勇³, 刘洋⁴, 刘佳⁵, 单贵莲¹, 初晓辉¹

1. 云南农业大学草业科学系, 云南昆明 650201;
2. 寻甸县农业农村局土壤肥料工作站, 云南寻甸 655200;
3. 云南省昆明市动物疫病预防控制中心, 云南昆明 650223;
4. 云南省林业与草原局, 云南昆明 650224;
5. 香格里拉市林业和草原局, 云南迪庆 674499

通讯作者: 单贵莲,E-mail:shanguilian8203@126.com;初晓辉,E-mail:478491637@qq.com
作者简介:罗钦(1998-),男,汉族,云南宣威人,硕士研究生,主要从事毒害草化感作用研究,E-mail:2680142652@qq.com;马祖艳(1997-),女,汉族,云南红河人,硕士研究生,主要从事毒害草化感作用研究,E-mail:948317080@qq.com
基金资助:
云南省农业联合专项（202101BD070001-134，202101BD070001-174）：云南省万人计划青年拔尖人才专项项目资助

Abstract

Abstract: In order to investigate the allelopathy and physiological characteristics of high quality forage induced by toxic plant Euphorbia jolkinii,we used high quality forage perennial ryegrass (Lolium perenne) as acceptor material and cultured with petri dish filter paper method and hole plate method to study the effects of the extract of E. jolkinii on the seed germination,seedling growth,physiological and endogenous hormone contents of perennial ryegrass. The results showed that the extract of E. jolkinii root,stem and leaf had significant allelopathic inhibition on seed germination and seedling growth of perennial ryegrass (P<0.05),and there were site differences and “concentration effect” on the inhibitory effect. The inhibitory strength of root extract was greater than that of stem and leaf extract,and The high concentration extract was greater than the low concentration extract. Under the allelopathy of the extracts from the root,stem and leaf of E. jolkinii,the content of MDA increased,the activities of antioxidant enzymes SOD and CAT increased,the content of endogenous hormone ABA increased,the contents of GA,IAA,ZT,JA and SA decreased,and the growth of perennial ryegrass seedlings was significantly inhibited (P<0.05).

Key words: Euphorbia jolkinii, Perennial ryegrass, Seed Germination, Seedling growth, Antioxidant enzymes, Endogenous hormone, Allelopathic effect

摘要： 为探讨有毒植物大狼毒（Euphorbia jolkinii）对优良牧草种子萌发及幼苗生长的化感效应，并从生理学层面揭示其作用机制，本试验以优良牧草多年生黑麦草（Lolium perenne）为受体材料，采用培养皿滤纸法和穴盘培养法，开展大狼毒浸提液对多年生黑麦草种子萌发、幼苗生长、生理及内源激素含量影响的研究，结果表明：大狼毒根、茎叶浸提液对多年生黑麦草种子萌发和幼苗生长均产生明显的化感抑制作用（P<0.05），抑制效果存在部位差异和“浓度效应”，具体表现为根浸提液抑制强度大于茎叶浸提液，高浓度浸提液抑制强度大于低浓度浸提液。在大狼毒根、茎叶浸提液的化感作用下，多年生黑麦草幼苗MDA含量升高，抗氧化酶SOD和CAT活性增强，内源激素ABA含量升高，GA，IAA，ZT，JA，SA含量下降，多年生黑麦草幼苗的生长受到显著抑制（P<0.05）。

关键词: 大狼毒, 多年生黑麦草, 种子萌发, 幼苗生长, 抗氧化酶, 内源激素, 化感效应

CLC Number:

S330.2

LUO Qin, MA Zu-yan, NIU Qiong-mei, GUI Bao-lin, XIE Yong, LIU Yang, LIU Jia, SHAN Gui-lian, CHU Xiao-hui. Allelopathic Effect and Physiological Mechanism of Extracts from different Parts of Euphorbia jolkinii on Growth of Loium Perenne Seedlings[J]. Acta Agrestia Sinica, 2023, 31(10): 3212-3219.

罗钦, 马祖艳, 牛琼梅, 桂宝林, 谢勇, 刘洋, 刘佳, 单贵莲, 初晓辉. 大狼毒不同部位浸提液对多年生黑麦草幼苗生长的化感效应及生理机制[J]. 草地学报, 2023, 31(10): 3212-3219.

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Allelopathic Effect and Physiological Mechanism of Extracts from different Parts of Euphorbia jolkinii on Growth of Loium Perenne Seedlings

大狼毒不同部位浸提液对多年生黑麦草幼苗生长的化感效应及生理机制

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