Effect of Lipopolysaccharide in Erwinia persicina Cp2 on the growth of Medicago sativa seedlings

doi:10.11733/j.issn.1007-0435.2023.02.036

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (2): 615-622.DOI: 10.11733/j.issn.1007-0435.2023.02.036

Effect of Lipopolysaccharide in Erwinia persicina Cp2 on the growth of Medicago sativa seedlings

ZHANG Hong, HUANG Rong, YAO Bo, ZHANG Yu-juan, ZHANG Zhen-fen

Pratacultural College, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2022-08-17 Revised:2022-09-26 Published:2023-02-28

桃色欧文氏菌Cp2脂多糖对紫花苜蓿幼苗生长的影响

张宏, 黄荣, 姚博, 张玉娟, 张振粉

甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 张振粉,E-mail:zhangzf@gsau.edu.cn
作者简介:张宏(1997-),男,土族,甘肃天祝人,硕士研究生,主要从事牧草病理学相关研究,E-mail:1510517949@qq.com
基金资助:
国家自然科学基金(32060369)；兰州市人才创新创业项目(2019-RC-116)；甘肃农业大学青年导师基金项目(GAU-QDFC-2019-08)；甘肃省杰出青年基金(20JR10RA562)；草业生态系统教育部重点实验室-人才培育项目(KLGE202203)资助

Abstract

Abstract: To investigate the effect of Erwinia persicina Cp2 Lipopolysaccharides (Cp2-LPSe) on the growth of alfalfa seedlings,in this experiment,alfalfa 'Juneng 551' was used to extract and purify Cp2-LPSe based on the improved hot phenol water method. Alfalfa was treated with 0,0.157,0.314,0.471,and 0.628 EU·mL^-1 Cp2-LPSe,and seed germination and seedling growth were investigated. The results showed that the average yield of Cp2-LPSe was 4.27%. The germination percentage,germination potential,and germination index of alfalfa seeds treated with different Cp2-LPSe concentrations were lower than those of the control,and the germination index was significantly lowered (P<0.05). With the increase of Cp2-LPSe concentration,all the growth indexes of seedlings increased at first and then decreased. With 0.157 EU·mL^-1 Cp2-LPSe treated,all the germination and growth indexes reached the maximum and were significantly higher than those of the control (P<0.05). The correlation and principal component analysis confirmed that 0.157 EU·mL^-1 Cp2-LPSe had a significant effect on the growth of alfalfa seedlings. In conclusion,0.157 EU·mL^-1 Cp2-LPSe treatment promoted the growth of alfalfa seedlings,and was beneficial to the accumulation of biomass,but more than this concentration would inhibit the growth. This study laid a practical and theoretical foundation for the development of alfalfa growth-promoting reagent Cp2-LPSe.

Key words: Erwinia persicina Cp2 lipopolysaccharides, Medicago sativa, Seedling growth, Correlation analysis

摘要： 为探究桃色欧文氏菌脂多糖(Erwinia persicina Cp2 lipopolysaccharides,Cp2-LPSe)对紫花苜蓿(Medicago sativa)幼苗生长的影响，本研究以紫花苜蓿'巨能551'为材料，用改良的热酚水法提取纯化Cp2-LPSe，设不同浓度(0，0.157，0.314，0.471和0.628 EU·mL^-1) Cp2-LPSe处理，测定处理后种子发芽和幼苗生长指标。结果表明：改良后的热酚水法提取Cp2-LPSe的平均产率为4.27%；不同Cp2-LPSe浓度下，紫花苜蓿种子的发芽率、发芽势和发芽指数均低于对照，且发芽指数显著低于对照(P<0.05)；随着Cp2-LPSe浓度的升高，幼苗的各生长指标均呈先升后降趋势，当Cp2-LPSe浓度为0.157 EU·mL^-1时，各指标均达到最大值，且显著高于对照(P<0.05)。相关性和主成分分析结果表明,0.157 EU·mL^-1Cp2-LPSe对紫花苜蓿幼苗生长有较大影响。综上，0.157 EU·mL^-1 Cp2-LPSe对紫花苜蓿幼苗生长有促进作用，而大于此浓度紫花苜蓿生长就会受到抑制。本研究为开发紫花苜蓿促生Cp2-LPSe制剂奠定了实践和理论基础。

关键词: 桃色欧文氏菌脂多糖, 紫花苜蓿, 幼苗生长, 相关性分析

CLC Number:

S963.22+3.3

ZHANG Hong, HUANG Rong, YAO Bo, ZHANG Yu-juan, ZHANG Zhen-fen. Effect of Lipopolysaccharide in Erwinia persicina Cp2 on the growth of Medicago sativa seedlings[J]. Acta Agrestia Sinica, 2023, 31(2): 615-622.

张宏, 黄荣, 姚博, 张玉娟, 张振粉. 桃色欧文氏菌Cp2脂多糖对紫花苜蓿幼苗生长的影响[J]. 草地学报, 2023, 31(2): 615-622.

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Effect of Lipopolysaccharide in Erwinia persicina Cp2 on the growth of Medicago sativa seedlings

桃色欧文氏菌Cp2脂多糖对紫花苜蓿幼苗生长的影响

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