Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch

doi:10.11733/j.issn.1007-0435.2023.05.005

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (5): 1314-1321.DOI: 10.11733/j.issn.1007-0435.2023.05.005

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Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch

TANG Ruo-yi¹, WANG Bai-sen², DONG Chun-xin¹, NIU Qi-chen¹, YIN Shu-xia¹

1. School of Grassland Science, Beijing Forestry University, Beijing 100083, China;
2. School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Received:2022-11-25 Revised:2023-01-22 Online:2023-05-15 Published:2023-05-31

棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探

唐若怡¹, 王柏森², 董纯辛¹, 牛启尘¹, 尹淑霞¹

1. 北京林业大学草业与草原学院, 北京 100083;
2. 北京林业大学园林学院, 北京 100083

通讯作者: 尹淑霞,E-mail:yinsx369@bjfu.edu.cn
作者简介:唐若怡(1997-)，女，汉族，山东枣庄人，硕士研究生，主要从事木霉生防作用研究，E-mail：TRY669@163.com
基金资助:
雄安新区科技创新专项(2022XAGG0100);国家林业和草原局委托项目(2021045001)资助

Abstract

Abstract: In order to explore the resistance mechanism of perennial ryegrass (Lolium perenne L.),induced by Trichoderma asperellum strain 152-42 against to brown patch,this study used both plateco-culture test and pot infection test to check the biocontrol effects of T. asperellum strain 152-42 against perennial ryegrass brown patch caused by Rhizoctonia zeae,and further analyze the resistance mechanism. The results showed that T. asperellum 152-42 could totally inhibit the R. zeae when they co-cultured at 7 days on PDA medium. Moreover,T. asperellum 152-42 significantly reduced the incidence of brown patch to perennial ryegrass. Compared to treatments that inoculated R. zeae but non-treated with T. asperellum 152-42,the diseased leaves treated with T. asperellum 152-42 displayed a significant increment of the superoxide dismutase(SOD) activity and a significant decrease of the Malondialdehyde (MDA) content,and higher expression of disease-resistance genes PR-1 and PR-5. In conclusion,T. asperellum 152-42 was able not only to significantly inhibit the growth of R. zeae,but also induced perennial ryegrass to improve resistance against the brown patch by increasing the activity of related enzymes and regulating related genes of disease-resistance. Therefore,the T. asperellum 152-42 is an excellent potential biocontrol fungus that can be used to control turf diseases of brown patch.

Key words: Trichoderma asperellum, Rhizoctonia zeae, Perennial ryegrass, Biological control, Turf brown patch

摘要： 为探究棘孢木霉(Trichoderma asperellum)诱导多年生黑麦草(Lolium perenne L.)抗褐斑病的机理,本研究以多年生黑麦草为材料,通过棘孢木霉和玉蜀黍丝核菌(Rhizoctonia zeae)平板对抗、盆栽多年生黑麦草木霉灌根诱导后接种玉蜀黍丝核菌,检测棘孢木霉菌株152-42对玉蜀黍丝核菌引起的褐斑病的防治效果,分析其防病机理。结果表明:共培养7 d时棘孢木霉152-42对玉蜀黍丝核菌的抑制率达到100%;棘孢木霉152-42显著降低了盆栽多年生黑麦草褐斑病的发病率;相较于未经木霉诱导的染病叶片,木霉诱导处理的染病叶片超氧化物歧化酶活性显著升高,丙二醛含量显著降低,抗病相关基因PR-1和PR-5的表达量显著提高。因此,棘孢木霉152-42不仅能显著抑制玉蜀黍丝核菌生长,而且可以诱导多年生黑麦草通过提高抗病相关酶活性,调控抗病相关基因的表达等提高抗病性,是一种极具开发潜力的草坪病害生防真菌。

关键词: 棘孢木霉, 玉蜀黍丝核菌, 多年生黑麦草, 生物防治, 褐斑病

CLC Number:

S543+ .6

TANG Ruo-yi, WANG Bai-sen, DONG Chun-xin, NIU Qi-chen, YIN Shu-xia. Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch[J]. Acta Agrestia Sinica, 2023, 31(5): 1314-1321.

唐若怡, 王柏森, 董纯辛, 牛启尘, 尹淑霞. 棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探[J]. 草地学报, 2023, 31(5): 1314-1321.

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References

[1] 马国胜,潘文明. 草坪常见病害及其综合防治技术[J]. 中国园林,2004,20(8):72-74
[2] 张馨馨,刘璐,陆妮,等. 叶面喷施生长延缓剂对多年生黑麦草生长及生理的影响[J]. 草原与草坪,2020,40(4):47-53
[3] 马敏芝. 多年生黑麦草-内生真菌共生体抗病性及其对根腐离蠕孢(Bipolaris sorokiniana)抗病机制的研究[D]. 兰州:兰州大学,2015:1
[4] VAJNA L,OROS G. First report of Rhizoctonia zeae on Lolium perenne and Festuca sp. in Hungary[J]. Plant Pathology,2005,54(2):250
[5] 陈海波,刘荣堂,杜广真,等. 草坪草褐斑病的研究进展和现状[J]. 草原与草坪,2002(3):10-14
[6] SOOD M,KAPOOR D,KUMAR V,et al. Trichoderma:The "secrets" of a multitalented biocontrol agent[J]. Plants,2020,9(6):762
[7] ZHANG J,CHEN G Y,LI X Z,et al. Phytotoxic,antibacterial,and antioxidant activities of mycotoxins and other metabolites from Trichoderma sp[J]. Natural Product Research,2017,31(23):2745-2752
[8] CAI X,ZHAO H,LIANG C,et al. Effects and mechanisms of symbiotic microbial combination agents to control tomato Fusarium crown and root rot disease[J]. Frontiers in Microbiology,2021,12:629793
[9] VINALEI F,SIVASITHAMPARAM K. Beneficial effects of Trichoderma secondary metabolites on crops[J]. Phytotherapy Research,2020,34(11):2835-2842
[10] WU Q,ZHANG L,XIA H,et al. Omics for understanding synergistic action of validamycin A and Trichoderma asperellum GDFS1009 against maize sheath blight pathogen[J]. Scientific Reports,2017,7(1):1-11
[11] SINGH R K,KUMAR P,TIWARI N N,et al. Role of Endochitinase gene and efficacy of Trichoderma against Colletotrichum falcatum Went. causing red rot disease in sugarcane[J]. Sugar Tech,2014,16(2):180-188
[12] GERALDINE A M,LOPES F A C,CARVALHO D D C,et al. Cell wall-degrading enzymes and parasitism of sclerotia are key factors on field biocontrol of white mold by Trichoderma spp[J]. Biological Control,2013,67(3):308-316
[13] 董纯辛,崔艺,牛启尘,等. 棘孢木霉特性及其对两种草坪病原菌的生防作用[J]. 草地学报,2022,30(5):1102-1109
[14] 许天委,郝慧华,吴小霞,等. 海南省高尔夫球场草坪真菌病害调查及防治[J]. 河南农业科学,2017,46(12):85-90
[15] SUDISHA J,NIRANJANA S R,UMESHA S,et al. Transmission of seed-borne infection of muskmelon by Didymella bryoniae and effect of seed treatments on disease incidence and fruit yield[J]. Biological Control,2006,37(2):196-205
[16] 李忠光,龚明. 植物生理学综合性和设计性实验的考核方法[J]. 植物生理学通讯,2008,44(3):551-553
[17] RAHMAN A,KULDAU G A,UDDIN W. Induction of salicylic acid-mediated defense response in perennial ryegrass against infection by Magnaporthe oryzae[J]. Phytopathology,2014,104(6):614-623
[18] LIVAK K J,SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J]. methods,2001,25(4):402-408
[19] HEND A A,KAHKASHAN P,RANIA T,et al. Evaluation of biological control potential of locally isolated antagonist fungi against Fusarium oxysporum under in vitro and pot conditions[J]. African Journal of Microbiology Research,2012,6(2):312-319
[20] RAHMAN S M M,MANIRUZZAMAN S M,Nusrat S,et al. In vitro evaluation of botanical extract,bioagents and fungicides against purple blotch diseases of bunch onion in Bangladesh[J]. Advances in zoology and botany,2015,3(4):179-183
[21] 杨萍,杨谦. 木霉重寄生过程分子机制的研究进展[J]. 中国农学通报,2012,28(27):163-166
[22] 邹佳迅,范晓旭,宋福强. 木霉(Trichoderma spp.)对植物土传病害生防机制的研究进展[J]. 大豆科学,2017,36(6):970-977
[23] 尹月兰,牛启尘,甘露. 绿木霉对币斑病菌的生长抑制作用及对镉的耐受性(英文)[J]. 草地学报,2022,30(10):2802-2810
[24] KUC J. Induced systemic resistance in plants to diseases caused by fungi and bacteria[J]. The Dynamics of Host Defense,1983:191-221
[25] SHORESH M,HARMAN G E,MASTOURI F. Induced systemic resistance and plant responses to fungal biocontrol agents[J]. Annual Review of Phytopathology,2010,48(1):21-43
[26] SINGH H B,SINGH B N,SINGH S P,et al. Solid-state cultivation of Trichoderma harzianum NBRI-1055 for modulating natural antioxidants in soybean seed matrix[J]. Bioresource Technology,2010,101(16):6444-6453
[27] JONES J D G,DANGL J L. The plant immune system[J]. Nature,2006,444(7117):323-329
[28] VINALE F,SIVASITHAMPARAM K,GHISALBERTI E L,et al. Trichoderma-plant-pathogen interactions[J]. Soil Biology and Biochemistry,2008,40(1):1-10
[29] 邱金龙,金巧玲,王钧. 活性氧与植物抗病反应[J]. 植物生理学通讯,1998,34(1):56-63
[30] 王晓瑜,丁婷婷,李彦忠,等. AM真菌与根瘤菌对紫花苜蓿镰刀菌萎蔫和根腐病的影响[J]. 草业学报,2019,28(8):139-149
[31] 李应德,段廷玉. AM真菌对紫花苜蓿茎点霉叶斑病及豌豆蚜为害的影响[J]. 生态学杂志,2020,39(4):1214-1221
[32] CHEN L H,YANG S L,CHUNG K R. Resistance to oxidative stress via regulating siderophore-mediated iron acquisition by the citrus fungal pathogen Alternaria alternata[J]. Microbiology,2014,160(5):970-979
[33] YOUSSEF S A,TARTOURA K A,ABDELRAOUF G A. Evaluation of Trichoderma harzianum and Serratia proteamaculans effect on disease suppression,stimulation of ROS-scavenging enzymes and improving tomato growth infected by Rhizoctonia solani[J]. Biological Control,2016,100:79-86
[34] KUMAR K,THAKUR P,RATHORE U S,et al. Plant beneficial effects of Trichoderma spp. suppressing Fusarium wilt and enhancing growth in Tomato[J]. Vegetos,2022,35(1):188-195
[35] VAN LOON L C,REP M,PIETERSE C M J. Significance of inducible defense-related proteins in infected plants[J]. Annual Review of Phytopathology,2006,44:135-162
[36] BREEN S,WILLIAMS S J,OUTRAM M,et al. Emerging insights into the functions of pathogenesis-related protein 1[J]. Trends in Plant Science,2017,22(10):871-879
[37] XIE X Z,XUE Y J,ZHOU J J,et al. Phytochromes regulate SA and JA signaling pathways in rice and are required for developmentally controlled resistance to Magnaporthe grisea[J]. Molecular Plant,2011,4(4):688-696
[38] ALI S,GANAI B A,KAMILI A N,et al. Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance[J]. Microbiological Research,2018,212:29-37
[39] MITSUHARA I,IWAI T,SEO S,et al. Characteristic expression of twelve rice PR1 family genes in response to pathogen infection,wounding,and defense-related signal compounds (121/180)[J]. Molecular Genetics and Genomics,2008,279(4):415-427
[40] El-KEREAMY A,El-SHARKAWY I,RAMAMOORTHY R,et al. Prunus domestica pathogenesis-related protein-5 activates the defense response pathway and enhances the resistance to fungal infection[J]. PLoS One,2011,6(3):e17973
[41] ZHANG Y,YAN H,WEI X,et al. Expression analysis and functional characterization of a pathogen-induced thaumatin-like gene in wheat conferring enhanced resistance to Puccinia triticina[J]. Journal of Plant Interactions,2017,12(1):332-339
[42] YAN X,QIAO H,ZHANG X,et al. Analysis of the grape (Vitis vinifera L.) thaumatin-like protein (TLP) gene family and demonstration that TLP29 contributes to disease resistance[J]. Scientific Reports,2017,7(1):1-14
[43] SAXENA A,MISHRA S,RAY S,et al. Differential reprogramming of defense network in Capsicum annum L. plants against Colletotrichum truncatum infection by phyllospheric and rhizospheric Trichoderma strains[J]. Journal of Plant Growth Regulation,2020,39(2):751-763
[44] YOSHIOKA Y,ICHIKAWA H,NAZNIN H A,et al. Systemic resistance induced in Arabidopsis thaliana by Trichoderma asperellum SKT-1,a microbial pesticide of seedborne diseases of rice[J]. Pest Management Science,2012,68(1):60-66

Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch

棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探

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