Effects of Fungal-enzyme Compound on the Quality of Vine Leaf Silage and the In vitro gas Production Characteristics of Rumen

doi:10.11733/j.issn.1007-0435.2024.10.034

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (10): 3320-3325.DOI: 10.11733/j.issn.1007-0435.2024.10.034

Effects of Fungal-enzyme Compound on the Quality of Vine Leaf Silage and the In vitro gas Production Characteristics of Rumen

HUO Zhi-wei¹, LI Hai-feng², YAN Yun-feng^1,3, ZHENG Xiao-kai¹, LI Shuang-ming¹, LIU Guo-hong², ZHANG Fan-fan¹

1. School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China;
2. Turpan Institute of Agricultural Science, Xinjiang Academy of Agricultural Sciences, Turpan, Xinjiang 838000, China;
3. Jinzhou Vocational and Technical Education Center, Jinzhou, Hebei Province 052260, China

Received:2024-05-17 Revised:2024-07-24 Published:2024-11-04

菌酶复合对葡萄枝叶青贮品质和瘤胃体外产气特性的影响

霍志伟¹, 李海峰², 闫云峰^1,3, 郑晓凯¹, 李双明¹, 刘国宏², 张凡凡¹

1. 石河子大学动物科技学院, 新疆石河子 832000;
2. 新疆农业科学院吐鲁番农业科学研究所, 新疆吐鲁番 838000;
3. 晋州市职业技术教育中心, 河北晋州 052260

通讯作者: 张凡凡,E-mail:zhangfanfan@shzu.edu.cn;刘国宏,E-mail:lghdjs@163.com
作者简介:霍志伟(2000-)，男，汉族，新疆沙湾人，硕士研究生，主要从事牧草生产与加工研究，E-mail:826047623@qq.com
基金资助:
吐鲁番市重点研发专项“吐鲁番市特色林果副产物饲料化微贮技术研究”;农业农村部国家现代农业产业技术体系项目(CARS-34)资助

Abstract

Abstract: The purpose of this study was to investigate the effects of combined addition of Lactiplantibacillus plantarum and cellulase on the fermentation quality of Vitis L. branch and leaf silage,to provide a basis for the feed utilization of grape branch and leaf resources. Three treatments were set up,CK treatment (without adding any fungicide),PL treatment (adding Lactobacillus plantarum 5×10⁵ cfu·g^-1 FM),and PLC treatment (adding Lactobacillus plantarum 5×10⁵ cfu·g^-1 FM and cellulase 0.3%FM). After 60 days of fermentation,the nutrients,fermentation quality and the number of main microorganisms were detected by opening the bag,and the gas production characteristics were evaluated by an In vitro fermentation test. The results showed that:1) Compared to the CK treatment,the contents of lactic acid,acetic acid and the number of lactic acid bacteria in PLC treatment were significantly increased (P<0.05),and the content and pH value of acid detergent fiber,yeast and aerobic bacteria were significantly decreased (P<0.05). 2) At the 60th day of silage,the degradation rate of organic matter,gas production and gas production rate of PLC treatment were the highest. It can be seen that the combined addition of Lactobacillus plantarum and cellulase can significantly improve the fermentation quality of grape branch and leaf silage,reduce nutrient loss,and improve feeding value.

Key words: Grape branches and leaves, Lactiplantibacillus plantarum, Cellulase, Fermentation quality, In vitro gas production characteristics

摘要： 本研究旨在探究联合添加植物乳杆菌(Lactiplantibacillus plantarum)与纤维素酶(cellulase)对葡萄(Vitis L.)枝叶青贮发酵品质的改善效果，为葡萄枝叶资源饲料化利用提供依据。试验设置3个处理，CK处理(不添加任何菌剂)、PL处理(添加植物乳杆菌5×10⁵ cfu·g^-1 FM)、PLC处理(添加植物乳杆菌5×10⁵ cfu·g^-1 FM和纤维素酶0.3%FM)。发酵60 d后开袋检测营养、发酵品质和主要微生物数量，进行体外发酵试验评定其产气特性。结果表明：(1)相比于CK处理，PLC处理中乳酸(Lactic acid，LA)、乙酸(Acetic acid,AA)含量和乳酸菌(Lactic acid bacteria,LAB)数量显著增加(P<0.05)，酸性洗涤纤维(Acid detergent fiber,ADF)含量和pH值、酵母菌(Yeast)和好氧细菌(Aerobic bacteria,AB)数量显著降低(P<0.05)。(2)在青贮第60 d时PLC处理的有机物降解率、产气量和产气速率最高。由此可见，联合添加植物乳杆菌与纤维素酶能够显著改善葡萄枝叶青贮发酵品质，减少营养物质损耗，提高饲喂价值。

关键词: 葡萄枝叶, 植物乳杆菌, 纤维素酶, 发酵品质, 产气特性

CLC Number:

S816.53

HUO Zhi-wei, LI Hai-feng, YAN Yun-feng, ZHENG Xiao-kai, LI Shuang-ming, LIU Guo-hong, ZHANG Fan-fan. Effects of Fungal-enzyme Compound on the Quality of Vine Leaf Silage and the In vitro gas Production Characteristics of Rumen[J]. Acta Agrestia Sinica, 2024, 32(10): 3320-3325.

霍志伟, 李海峰, 闫云峰, 郑晓凯, 李双明, 刘国宏, 张凡凡. 菌酶复合对葡萄枝叶青贮品质和瘤胃体外产气特性的影响[J]. 草地学报, 2024, 32(10): 3320-3325.

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Effects of Fungal-enzyme Compound on the Quality of Vine Leaf Silage and the In vitro gas Production Characteristics of Rumen

菌酶复合对葡萄枝叶青贮品质和瘤胃体外产气特性的影响

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