Abstract: Abiotic stress restricts the growth and development of oat to a certain extent. Cloning and expression analysis of oat key genes can lay a theoretical foundation for improving its stress resistance. In this study,we cloned the AsGRAS36 gene from oat,the N-terminal end of the AsGRAS36 protein contains DELLA and TVHYNP domains that belongs to the DELLA protein. The analysis of protein physicochemical properties showed that the AsGRAS36 encoded 611 amino acids,with a protein molecular weight of 64.81 kD and a theoretical isoelectric point of 4.97. It is a hydrophilic protein,which mainly consists of α-helix and random coil. Phylogenetic analysis suggested that the AsGRAS36 is the most closely related to GRAS28 in barley and GRAS115 in wheat. Cis-acting element analysis showed that the promoter region of the AsGRAS36 gene contains hormone response elements such as abscisic acid and salicylic acid,as well as stress response elements such as drought and low temperature. The results of protein-protein interaction prediction indicated that the main proteins that interacted with AsGRAS36 were involved in the stress response process in plants. Gene expression analysis showed that AsGRAS36 was rapidly increased after abiotic stress and responded sensitively to high temperature,drought and hormone stresses. The results of this study provide a scientific basis for the role of AsGRAS36 gene and DELLA protein in the regulation of abiotic stress in oat.

Key words: Avena sativa, DELLA protein, Abiotic stress, Expression analysis

摘要： 非生物胁迫在一定程度上制约了燕麦(Avena sativa L.)的生长和发育，其关键基因的克隆和表达分析可为提高植物逆境耐受性奠定理论基础。本研究从燕麦叶片中克隆到AsGRAS36基因，该蛋白N端含有DELLA结构域和TVHYNP结构域，确定其属于DELLA蛋白。蛋白理化性质分析表明AsGRAS36编码611个氨基酸，蛋白质相对分子质量为64.81 kD，理论等电点为4.97，为亲水性蛋白，主要由α-螺旋和无规则卷曲组成。系统进化分析显示AsGRAS36与大麦GRAS28和小麦GRAS115的亲缘关系最近。顺式作用元件分析揭示AsGRAS36基因启动子含有脱落酸和水杨酸等激素响应元件和干旱、低温等胁迫响应元件。蛋白互作预测显示与AsGRAS36互作的蛋白参与到植物的胁迫响应过程。基因表达分析表明AsGRAS36在燕麦受到非生物胁迫后迅速表达，对高温、干旱等胁迫和激素诱导响应敏感。该研究结果为AsGRAS36基因及DELLA蛋白在燕麦非生物胁迫调控中的作用提供了科学依据。

关键词: 燕麦, DELLA蛋白, 非生物胁迫, 表达分析