Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (11): 3451-3461.DOI: 10.11733/j.issn.1007-0435.2024.11.013

Previous Articles    

Study on Physiological and Biochemical Changes of Paeonia lactiflora Seed During Dormancy Breaking

MU Ying-tong1, LYU Li-juan2, LI Xiao-jie1, WANG Jun-jie1, LU Jing-shi1, JIA Jun-ying3, QING Ge-le4, SANG Hao5, ZHANG Xiao-ming1   

  1. 1. Inner Mongolia Autonomous Region Sino-Mongolia Medicinal Materials Breeding Engineering Technology Research Center, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010019, China;
    2. Hohhot Forestry and Grassland Bureau, Hohhot, Inner Mongolia 010019, China;
    3. Universities Engineering Research Center of Chinese (Mongolia) Ecological Planting Medicinal Materials (Nurture) in Inner Mongolia Autonomous Region, College of Agronomy, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000, China;
    4. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot Inner Mongolia 010031, China;
    5. Inner Mongolia Academy of Forestry, Hohhot, Ineer Mongolia 010031, China
  • Received:2024-01-09 Revised:2024-03-26 Published:2024-11-29

芍药种子层积过程中生理生化变化研究

穆赢通1, 吕丽娟2, 李晓杰1, 王俊杰1, 路景诗1, 贾俊英3, 青格乐4, 桑昊5, 张晓明1   

  1. 1. 内蒙古农业大学草原与资源环境学院/内蒙古自治区中蒙药材良种育繁工程技术研究中心, 内蒙古 呼和浩特 010019;
    2. 呼和浩特市林业和草原局, 内蒙古 呼和浩特 010019;
    3. 内蒙古民族大学农学院/内蒙古自治区高校中(蒙)药材生态 种植工程研究中心(培育), 内蒙古 通辽 028000;
    4. 内蒙古自治区农牧业科学院, 内蒙古 呼和浩特 010031;
    5. 内蒙古自治区林业科学研究院, 内蒙古 呼和浩特 010031
  • 通讯作者: 张晓明,E-mail:bagenna123@aliyun.com
  • 作者简介:穆赢通(1997-),男,汉族,河南驻马店人,硕士研究生,主要从事药用植物资源鉴定研究,E-mail:2911426494@qq.com
  • 基金资助:
    (财政资金)四种特色蒙药材种植技术集成配套与示范项目(RZ1900001339);兴安盟乡土植物种质资源收集、保存及评价;内蒙古自然科学基金(2019MS03052);六种特色蒙药材原生态种植关键技术研究(2021GG0327)资助

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Abstract: The accumulation process of Paeonia lactiflora seed lays the foundation for studying its dormancy mechanism by exploring its morphological changes,nutrient substances,enzyme activity,endogenous hormones,and the expression of key genes. The study investigated the morphological changes of seeds and measured the levels of nutrient substances,enzyme activity,hormones,and key genes expression. The process of dormancy release was divided into lower embryo axis growth,embryo bud swelling,and upper embryo axis growth;initial storage materials were primarily composed of fats,accounting for about 28%. During the dormancy release process,fats,starch,proteins,and soluble sugars decreased overall,providing substances for germination. Fats were consumed the most during dormancy release,serving as the main source of energy substances. The changes in enzyme activity during the process of dormancy release reflected the trend of nutrient changes,with an overall enhancement in antioxidant enzyme activity. During dormancy release,there was an initial increase,followed by a decrease in gibberellic acid (GA) content,while the content of abscisic acid (ABA) and the GA/ABA ratio significantly decreased. Changes in key genes aligned with changes in endogenous hormone levels. The release of dormancy in Paeonia lactiflora seeds required fluctuations of temperature from warm to cold conditions,enhanced enzyme activity,and the breakdown of nutrients to provide energy for dormancy release. The significant decrease in ABA content was the main hormone inhibiting germination. Releasing dormancy in Paeonia lactiflora seeds required coordinated regulation of energy metabolism and hormones.

Key words: Paeonia lactiflora seeds, Seed dormancy, Seed morphology, Physiological and biochemical index

摘要： 芍药(Paeonia lactiflora)种子层积过程中形态、营养物质、酶活性、内源激素及关键基因的表达，为其休眠机制研究奠定基础。研究种子的形态变化，测定营养物质、酶活性、激素及关键基因的表达。休眠解除过程分为下胚轴生长、胚芽膨大和上胚轴生长;初始储藏物质以脂肪为主，约28%。休眠解除过程脂肪、淀粉、蛋白质和可溶性糖整体下降，为萌发提供物质。脂肪在休眠解除中消耗最多，为主要供能物质。休眠解除过程分解营养物质的酶活性变化符合营养物质变化趋势，抗氧化酶活性整体增强;休眠解除过程中GA含量呈现上升-下降，ABA含量和GA/ABA比值显著下降，关键基因变化符合内源激素含量变化。芍药种子休眠解除须满足暖温-低温的变化，酶活性增强，营养物质分解为休眠解除提供能量，ABA含量显著下降是抑制萌发的主要激素。芍药种子休眠的解除需要能量代谢和激素共同调控。

关键词: 芍药种子, 休眠, 种子形态, 生理生化指标

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