Transcriptome Sequencing and Their Differential Gene Expression Analysis of Sorghum sudanense (Piper) Stapf. and Sorghum bicolor (Linn.) Moench. × Sorghum sudanense (Piper) Stapf.

doi:10.11733/j.issn.1007-0435.2024.03.007

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (3): 714-725.DOI: 10.11733/j.issn.1007-0435.2024.03.007

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Transcriptome Sequencing and Their Differential Gene Expression Analysis of Sorghum sudanense (Piper) Stapf. and Sorghum bicolor (Linn.) Moench. × Sorghum sudanense (Piper) Stapf.

HONG Sen-rong^1,2,3, LIU Jia-ning¹, YUAN Xin¹, ZENG Zhi-yi¹, Mu Yesai·Turuhong¹, YANG Kai-tai¹, XIE Xin⁴

1. College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi Province 334001, China;
2. Shangrao Agricultural Technology Innovation Research Institute, Shangrao, Jiangxi Province 334001, China;
3. Jiangxi Province Modern Cow and Sheep Industry Technology System Gandong Comprehensive Experiment and Promotion Station, Shangrao, Jiangxi Province 334001, China;
4. Agriculture and Rural Bureau in Fuliang County, Jingdezhen, Jiangxi Province 333039, China

Received:2023-08-25 Revised:2023-09-16 Online:2024-03-15 Published:2024-04-03

苏丹草和高丹草转录组测序及其差异基因表达分析

洪森荣^1,2,3, 刘佳凝¹, 袁昕¹, 曾芷仪¹, 木也赛尔·吐鲁洪¹, 杨开泰¹, 谢欣⁴

1. 上饶师范学院生命科学学院, 江西上饶 334001;
2. 上饶农业技术创新研究院, 江西上饶 334001;
3. 江西省现代牛羊产业技术体系赣东综合试验推广站, 江西上饶 334001;
4. 浮梁县农业农村局, 江西景德镇 333039

通讯作者: 洪森荣,E-mail:hongsenrong@163.com
作者简介:洪森荣(1974-)，男，汉族，江西永新人，硕士，教授，主要从事草学生物技术研究，E-mail：hongsenrong@163.com
基金资助:
国家自然科学基金资助目(31960079)；江西省科技厅重点研发计划一般项目(20202BBG73010)；江西省教育厅科学技术研究项目(GJJ201704；GJJ211729)；江西省现代农业产业技术体系建设专项(JXARS-13-赣东站)；上饶市科技局平台载体建设项目(2020J001)资助

Abstract

Abstract: In order to preliminarily explore the differential gene expression between Sorghum sudanense (Piper) Stapf. and Sorghum bicolor (Linn.) Moench.×Sorghum sudanense (Piper) Stapf. Perform,their transcriptome was sequenced and the expression of differential genes related to tillering regulation,crude protein and lignin synthesis was analyzed in this study. The results showed that compared with S. sudanense (Piper) Stapf.,the number of tillers (NT) per plantlets of S. bicolor (Linn.) Moench.×S. sudanense (Piper) Stapf. Perform significantly reduced,and the contents of crude protein (CP),lignin (LN),and monolactone (SLs),as well as the activities of fructose kinase (FRK),phenylalanine ammonia lyase (PAL),polyphenol oxidase (PPO),trans cinnamate 4-monooxygenase (C4H) significantly increased. Hexokinase 3 (HXK3),fructose kinase 2 (FRK2) gene,aspartate aminotransferase (ASPAT) gene,S-adenosylmethionine synthetase 1 (SAMS1) gene,polyphenol oxidase II (PPOII) gene,bifunctional aspartate kinase/homoserine dehydrogenase 2 (AKI/DHI2) gene,phenylalanine ammonia lyase (PAL) gene,trans cinnamate 4-monooxygenase (C4H) gene upregulated,while the expression of the monolithine esterase D14 (SLsE D14) gene downregulated;This study lays the foundation for the cloning and molecular marker development of functional genes related to S. sudanense (Piper) Stapf. and S. bicolor (Linn.) Moench.×S. sudanense (Piper) Stapf. Perform.

Key words: Sorghum sudanense (Piper) Stapf., Sorghum bicolor × Sorghum sudanense hybrids, Transcriptome analysis, Tillering regulation, Protein synthesis, Lignin synthesis

摘要： 为了初步探明苏丹草[Sorghum sudanense (Piper) Stapf.]和高丹草[Sorghum bicolor (Linn.) Moench.×Sorghum sudanense (Piper) Stapf.]相关差异基因的表达,本研究对两者进行转录组测序,并对分蘖调控以及粗蛋白和木质素合成关联基因的差异表达进行分析。结果表明:与苏丹草相比,高丹草的单株分蘖数显著降低,粗蛋白、木质素和独角金内酯含量以及果糖激酶、苯丙氨酸解氨酶、多酚氧化酶、反肉桂酸4-单加氧酶活性显著提高,己糖激酶-3(HXK3)、果糖激酶2(FRK2)、天冬氨酸转氨酶(ASPAT)、S-腺苷甲硫氨酸合成酶1(SAMS1)、多酚氧化酶II(PPOII)、双功能天冬氨酸激酶/高丝氨酸脱氢酶2(AKI/DHI2)、苯丙氨酸解氨酶(PAL)、反-肉桂酸4-单加氧酶(C4H)基因表达上调,独角金内酯酯酶D14(SLsE D14)基因表达下调。本研究为苏丹草和高丹草相关功能基因的克隆、分子标记开发等工作奠定了基础。

关键词: 苏丹草, 高丹草, 转录组分析, 分蘖调控, 粗蛋白合成, 木质素合成

CLC Number:

S544

HONG Sen-rong, LIU Jia-ning, YUAN Xin, ZENG Zhi-yi, Mu Yesai·Turuhong, YANG Kai-tai, XIE Xin. Transcriptome Sequencing and Their Differential Gene Expression Analysis of Sorghum sudanense (Piper) Stapf. and Sorghum bicolor (Linn.) Moench. × Sorghum sudanense (Piper) Stapf.[J]. Acta Agrestia Sinica, 2024, 32(3): 714-725.

洪森荣, 刘佳凝, 袁昕, 曾芷仪, 木也赛尔·吐鲁洪, 杨开泰, 谢欣. 苏丹草和高丹草转录组测序及其差异基因表达分析[J]. 草地学报, 2024, 32(3): 714-725.

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Transcriptome Sequencing and Their Differential Gene Expression Analysis of Sorghum sudanense (Piper) Stapf. and Sorghum bicolor (Linn.) Moench. × Sorghum sudanense (Piper) Stapf.

苏丹草和高丹草转录组测序及其差异基因表达分析

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