A Effects of Cellulase and High-temperature Tolerant Lactic Acid Bacteria on Structural Carbohydrate of (Pennisetum ameri-canum×P.purpureum)×P.durpureum schum.

doi:10.11733/j.issn.1007-0435.2024.07.033

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (7): 2314-2322.DOI: 10.11733/j.issn.1007-0435.2024.07.033

A Effects of Cellulase and High-temperature Tolerant Lactic Acid Bacteria on Structural Carbohydrate of (Pennisetum ameri-canum×P.purpureum)×P.durpureum schum.

WU Qi-feng¹, CHEN Chen¹, HUANG Qin-jiao¹, ZHANG Yun-fei¹, LI Xiao-mei¹, CHEN Yuan-hang¹, WEN Xing-jin², YAN Yan-hong¹

1. College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China;
2. Sichuan Academy of Grassland Science, Chengdu, Sichuan Province 6l1731, China

Received:2024-03-28 Revised:2024-05-10 Published:2024-08-03

纤维素酶和乳酸菌对杂交象草青贮结构性碳水化合物影响

武齐丰¹, 陈晨¹, 黄沁骄¹, 张云飞¹, 李小梅¹, 陈远航¹, 文兴金², 闫艳红¹

1. 四川农业大学草业科技学院, 四川成都 611130;
2. 四川省草原科学研究院, 四川成都 611731

通讯作者: 闫艳红，E-mail:yanyh@sicau.edu.cn
作者简介:武齐丰(1997-)，女，汉族，宁夏吴忠人，博士研究生，主要从事饲草生产与加工利用研究，E-mail：1018609775@qq.com
基金资助:
国家自然科学基金(32001401)；四川省科技厅国际合作项目(2021YFH0155)资助

Abstract

Abstract: In order to solve the problem of high crude fiber content of hybrid napier grass [(Pennisetum ameri-canum×P.purpureum)×P.durpureum schum. 'Guimu No.1’],cellulase and high-temperature resistant lactic acid bacteria (LAB) were used as additives to analyze their effects on structural carbohydrates of hybrid napier grass silage for 1,3,7,14 and 60 days at 45℃.The results showed that additive treatments had higher (P＜0.05) lactic acid content and lower (P＜0.05) pH value,acetic acid,propionic acid,and ammoniacal nitrogen contents. The commercia LAB group had the highest (P＜0.05) lactic acid and the lowest (P＜0.05) acetic acid contents. Neutral detergent fiber,acid detergent fiber,hemicellulose,and cellulose contents were lower (P＜0.05),whereas water-soluble carbohydrates,glucose,and sucrose contents were higher (P＜0.05) in all additive treatment groups. The cellulase group had the lowest (P＜0.05) content of fibrous components and the highest (P＜0.05) content of soluble carbohydrates,followed by the high-temperature resistant LAB group. In summary,the addition of cellulase and LAB to the napier silage could degrade structural carbohydrates and promote lactic acid fermentation,and the effects of cellulase was better.

Key words: (Pennisetum ameri-canum ×P.purpureum)×P.durpureum schum.'Guimu No.1’, Cellulase, Lactic acid bacteria, Fermentation quality, Structural carbohydrates

摘要： 为解决杂交象草[(Pennisetum ameri-canum×P.purpureum)×P.durpureum schum]青贮粗纤维含量高的难题，本研究以纤维素酶和耐高温乳酸菌为添加剂，45℃青贮1，3，7，14，60 d，解析其对杂交象草青贮料结构性碳水化合物影响。结果表明：添加剂处理组乳酸含量较高(P＜0.05)，pH值，乙酸，丙酸和氨态氮含量较低(P＜0.05)。商业乳酸菌组乳酸含量最高，乙酸含量最低(P＜0.05)。添加剂处理组的中性洗涤纤维、酸性洗涤纤维、半纤维素和纤维素含量较低(P＜0.05)，水溶性碳水化合物、葡萄糖和蔗糖含量较高(P＜0.05)。纤维素酶组的中性洗涤纤维、酸性洗涤纤维、半纤维素和纤维素含量最低(P＜0.05)，水溶性碳水化合物、葡萄糖和蔗糖含量最高(P＜0.05)，耐高温乳酸菌组次之。综上所述，在杂交象草青贮饲料中添加纤维素酶和乳酸菌均能降解结构性碳水化合物并促进乳酸发酵，其中以纤维素酶添加效果最佳。

关键词: ‘桂牧1号’杂交象草, 纤维素酶, 乳酸菌, 青贮发酵品质, 结构性碳水化合物

CLC Number:

S816.53

WU Qi-feng, CHEN Chen, HUANG Qin-jiao, ZHANG Yun-fei, LI Xiao-mei, CHEN Yuan-hang, WEN Xing-jin, YAN Yan-hong. A Effects of Cellulase and High-temperature Tolerant Lactic Acid Bacteria on Structural Carbohydrate of (Pennisetum ameri-canum×P.purpureum)×P.durpureum schum.[J]. Acta Agrestia Sinica, 2024, 32(7): 2314-2322.

武齐丰, 陈晨, 黄沁骄, 张云飞, 李小梅, 陈远航, 文兴金, 闫艳红. 纤维素酶和乳酸菌对杂交象草青贮结构性碳水化合物影响[J]. 草地学报, 2024, 32(7): 2314-2322.

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References

[1] 贾燕霞,玉柱,邵涛,等. 添加酶制剂对象草青贮发酵品质的影响[J]. 草地学报,2009,17(1):121-124
[2] 黄佩珊,张超,陈丹丹,等. 辣木叶提取物对柱花草和象草青贮品质的影响[J]. 草地学报,2023,31(7):2194-2202
[3] LI F H,KE W C,BAI J,et al. Pretreatment of Pennisetum sinese silages with ferulic acid esterase-producing lactic acid bacteria and cellulase at two dry matter Contents:Fermentation characteristics,carbohydrates composition and enzymatic saccharification[J]. Bioresource Technology,2020(295):122261
[4] 李莉,吴汉葵,解祥学,等. 添加纤维素酶和淀粉对象草青贮发酵品质的影响[J]. 动物营养学报,2021,33(9):5025-5035
[5] 赵金鹏. 添加剂对水稻秸秆青贮发酵品质及碳水化合物的影响[D]. 南京:南京农业大学,2018:3-8
[6] 姚娜,赖志强,滕少花,等. 桂闽引象草对肉鹅生长性能的影响[J]. 中国草地学报,2016,38(3):96-100
[7] 黄志朝,易显凤,庞天德,等. 紫色象草发酵料对桂科黑猪育肥效果分析[J]. 饲料研究,2021.44(13):35-39
[8] 何玮,冉启凡.添加纤维素酶和乳酸菌对'桂牧1号’杂交狼尾草青贮品质的影响[J]. 中国饲料,2022(3):129-132
[9] 赵苗苗,玉柱. 添加乳酸菌及纤维素酶对象草青贮品质的改善效果[J]. 草地学报,2015,23(1):205-210
[10] 李君风. 牦牛瘤胃中高效纤维素降解菌的分离,鉴定及其在青贮中的应用研究[D]. 南京：南京农业大学,2018:18-22
[11] KUNG L M,SHAVER D R,GRANTR J,et al. Silage review:Interpretation of chemical,microbial,and organoleptic components of silages[J]. Journal of Dairy Science,2018(101):4020-4033
[12] TABSCCO E,PIANO S,REVELLO A,et al. Effect of Lactobacillus buchneri LN4637 and Lactobacillus buchneri LN40177 on the aerobic stability,fermentation products,and microbial populations of corn silage under farm Conditions[J]. Journal of Dairy Science,2011(94):5589-5598
[13] YAN Y H,LI X L,GUAN H,et al. Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria[J]. Bioresource Technology,2019(79):166-173
[14] CHEN C,XIN Y F,LI X,et al. Effects of Acremonium cellulase and heat-resistant lactic acid bacteria on lignocellulose degradation,fermentation quality,and microbial community structure of hybrid elephant grass silage in humid and hot areas[J]. Frontiers in microbiology,2022(13):1066753
[15] KHOTA W,PHOLSEN S,HIGG D,et al. Natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant-ScienceDirect[J]. Journal of Dairy Science,2016(99):9768-9781
[16] BAI B,QIU R,WANG Z,et al. Effects of Cellulase and Lactic Acid Bacteria on Ensiling Performance and Bacterial Community of Caragana korshinskii Silage[J]. Microorganisms,2023(11):337
[17] 李福厚. 产阿魏酸酯酶乳酸菌对青贮饲料纤维降解、家畜消化及健康的影响及作用机制研究[D]. 兰州:兰州大学,2021:40-62
[18] 黄小云,陈长福,黄勤楼,等. 乳酸菌和纤维素酶对狼尾草和圆叶决明混合青贮品质的影响[J]. 畜牧与兽医,2022(54):2
[19] 关皓,曾泰儒,帅杨,等. 西南高温高湿地区青贮中天然乳酸菌群落结构特征及优质乳酸菌的筛选[J]. 草业科学,2019,36(12):202-212
[20] 刘秦华,张建国,卢小良. 乳酸菌添加剂对王草青贮发酵品质及有氧稳定性的影响[J]. 草业学报,2009,18(4):31-137
[21] BRODERICK G A,KANG J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science,1980(63):64-75
[22] ZONG C,WU Q,WU A,et al. Exploring the diversity mechanism of fatty acids and the loss mechanisms of polyunsaturated fatty acids and fat-soluble vitamins in alfalfa silage using different additives[J]. Animal Feed Science and Technology,2021(280):115044
[23] YAN Y H,LI J L,GUO X S,et al. A study on fermentation quality of Italian ryegrass and soybean straw mixed silage[J]. Acta Prataculturae Sinica,2014(4):38
[24] SOEST PJ VAN,ROBERTSON J B,LEWIS B A. Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science,1991(74):3583-3597
[25] LI F,DING Z,KE W,et al. Ferulic acid esterase-producing lactic acid bacteria and cellulase pretreatments of corn stalk silage at two different temperatures:Ensiling characteristics,carbohydrates composition and enzymatic saccharification[J]. Bioresource Technology,2019(282):211-221
[26] NI K,WANG F,ZHU B,et al. Effects of lactic acid bcteria and molasses additives on the microbial community and fermentation quality of soybean silage[J]. Bioresource Technology,2017(238):706-715
[27] 金丽娜,蒋苏苏,敬淑燕,等. 黄芪药渣与全株玉米混合青贮对饲料感官、发酵品质及营养成分的影响[J]. 草地学报,2024,32(2):630-636
[28] HE L,WANG C,XING Y,et al. Dynamics of proteolysis,protease activity and bacterial community of Neolamarckia cadamba leaves silage and the effects of formic acid and Lactobacillus farciminis[J]. Bioresource Technology,2019(294):122127
[29] MCDONALD P,HENDERSON A R,HERON S J E. The biochemistry of silage[M]. Marlow:Chalcombe Publication,1991:250-251
[30] WANG C,ZHENG M,WU S,et al. Effects of gallic acid on fermentation parameters,protein fraction,and bacterial community of whole plant soybean silage[J]. Frontiers in Microbiology,2021(12):662966
[31] NWS A L,ZHAO J,DONG Z H,et al. Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota[J]. Bioresource Technology,2020(314):123606
[32] XIONG H,ZHU Y,WEN Z,et al. Effects of cellulase, Lactobacillus plantarum,and sucrose on fermentation parameters,chemical composition,and bacterial community of hybrid Pennisetum silage[J]. Fermentation,2022(8):356
[33] 潘俊歆,李雪枫,龙仕和,等. 柱花草耐高温优质乳酸菌的筛选及对其青贮发酵品质的改善效果[J]. 草业科学,2024,41(2):1-12
[34] SILVA P V,PEREIRA G O,LEANDRO S E,et al. Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical Conditions[J]. Journal of Dairy Science,2016(99):1895-1902
[35] 王啸林,冯冠智,张城瑞,等. 乳酸菌和纤维素酶对马铃薯渣与玉米秸秆混合青贮发酵品质,有氧稳定性和体外发酵参数的影响[J]. 中国畜牧杂志,2022,58(4):201-207
[36] KLEINWSCHMIT D H,JR L K. A Meta-Analysis of the Effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages[J]. Journal of Dairy Science,2006(89):4005-4013
[37] LI J F,YUAN X J,SEARE D,et al. Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese[J]. Bioresource Technology,2018(2):70
[38] PITIWITTAYALUI N,BUREENOK S,SCHONEWILLE J T,et al. Selective thermotolerant lactic acid Bacteria isolated from fermented juice of epiphytic lactic acid bacteria and their effects on fermentation quality of stylo silages[J]. Frontiers in Microbiology,2021(12):673946
[39] 朱春娟,原现军,董志浩,等. 添加剂对饲料型四倍体刺槐青贮发酵品质和体外消化率的影响[J]. 南京农业大学学报,2017,40(4):725-731
[40] 黄莉莹,崔卫东,田静,等. 大豆秸和稻草对甜玉米秸秆和湿啤酒糟混合青贮的影响[J]. 草地学报,2024,32(3):945-951
[41] 吴征敏,彭苏,王志敬,等. 添加乳酸菌和纤维素酶对菠萝渣青贮发酵品质和体外发酵的评价[J]. 动物营养学报,2022,34(12):981-992
[42] MU L,XIE Z,HU L,et al. Cellulase interacts with Lactobacillus plantarum to affect chemical composition,bacterial communities,and aerobic stability in mixed silage of high-moisture amaranth and rice straw[J]. Bioresource Technology,2020(315):123772
[43] 陈凌华,杨志坚,程祖锌. 添加酶制剂和乳酸菌对水稻秸秆青贮质量的影响[J]. 中国饲料,2018(20):81-85
[44] KURNIASIH S D,ALFI A,NATALIA D,et al. Construction of individual,fused,and co-expressed proteins of endoglucanase and β -glucosidase for hydrolyzing sugarcane bagasse[J]. Microbiological Research,2014(169):725-732
[45] LI J F,YUAN X J,DONG Z H,et al. The effects of fibrolytic enzymes,cellulolytic fungi and bacteria on the fermentation characteristics,structural carbohydrates degradation,and enzymatic Conversion yields of Pennisetum sinese silage[J]. Bioresource Technology,2018(264):123-130
[46] ZHAO J,DONG Z H,LI J F,et al. Ensiling as pretreatment of rice straw:The effect of hemicellulase and Lactobacillus plantarum on hemicellulose degradation and cellulose conversion[J]. Bioresource Technology,2018(266):158-165

A Effects of Cellulase and High-temperature Tolerant Lactic Acid Bacteria on Structural Carbohydrate of (Pennisetum ameri-canum×P.purpureum)×P.durpureum schum.

纤维素酶和乳酸菌对杂交象草青贮结构性碳水化合物影响

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