Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow

doi:10.11733/j.issn.1007-0435.2025.01.002

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (1): 10-20.DOI: 10.11733/j.issn.1007-0435.2025.01.002

Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow

DUAN Rui¹, LUO Qin¹, XIAO Xue¹, NIU Qiong-mei¹, LIU Yang², YANG Xue¹, LU Jia-qi¹, CHU Xiao-hui¹, SHAN Gui-lian¹

1. Department of Grassland Science, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
2. Forestry and Grassland Bureau of Yunnan Province, Kunming, Yunnan Province 650224, China

Received:2024-07-15 Revised:2024-09-13 Published:2025-01-22

大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响

段蕊¹, 罗钦¹, 肖雪¹, 牛琼梅¹, 刘洋², 杨雪¹, 陆嘉琪¹, 初晓辉¹, 单贵莲¹

1. 云南农业大学草业科学系, 云南昆明 650201;
2. 云南省林业与草原局, 云南昆明 650224

通讯作者: 初晓辉,E-mail：478491637@qq.com；单贵莲,E-mail：shanguilian8203@126.com
作者简介:段蕊（2000-）,女,汉族,云南富源人,硕士研究生,主要从事毒害草化感作用研究,E-mail：gnahsoak@qq.com；罗钦（1998-）,男,汉族,云南宣威人,硕士研究生,主要从事毒害草化感作用研究,E-mail：2680142652@qq.com
基金资助:
云南省万人计划青年拔尖人才专项（YNWR-QNBJ-2018-031）；云南省农业联合专项（202101BD070001-068,202301BD070001-134,202301BD070001-174）；云南省水利科技项目（2023BG204001）资助

Abstract

Abstract: To investigate the regulatory role of Euphorbia jolkinii allelopathic substances in the invasion process of its population. In this study, we measured and analyzed the effects of addition of 4-Nitrophenol and 2-Phenylethylamine on soil microorganisms in the montane meadow by using artificially controlled addition of allelochemical solutions, indoor culture of soil samples, and macro-genome sequencing technology. The results showed that the addition of 4-Nitrophenol had no significant effect on the species composition, abundance and diversity of soil bacteria and fungi, while the addition of 2-Phenylethylamine altered the soil bacterial Acidobacteria, Proteobacteria,Candidatus_Nitrosotalea and Bradyrhizobium, and significantly increased soil bacterial species diversity and evenness, as well as fungal richness （P<0.05）. In conclusion, the addition of Euphorbia jolkinii 2 major allelochemical solutions increased the number of soil microbial species and promoted the growth and reproduction of soil microorganisms in the montane meadow, but there were differences in the effects of the different substances on the diversity of soil microorganisms, and the extent of the effect of 2-Phenylethylamine was even higher than that of 4-Nitrophenol.

Key words: Euphorbia jolkinii, Allelopathic substances addition, Soil microorganisms, Relative abundance of dominant bacteria, Species diversity

摘要： 为探讨大狼毒（Euphorbia jolkinii）化感物质在其种群入侵过程中的调节作用,本试验采用人为控制添加化感物质溶液、室内培养土壤样品和宏基因组测序技术,测定分析添加4-硝基苯酚和2-苯乙胺溶液对山地草甸土壤微生物的影响。结果表明：2种化感物质的添加均会显著增加土壤微生物种水平的物种数目；4-硝基苯酚添加对土壤细菌和真菌的物种组成、丰富度和多样性均无显著影响；2-苯乙胺添加会改变土壤细菌酸杆菌门（Acidobacteria）、变形菌门（Proteobacteria）、亚硝化杆菌属（Candidatus_Nitrosotalea）和慢生根瘤菌属（Bradyrhizobium）的相对丰度,显著增加土壤细菌物种多样性和均匀度,以及真菌丰富度（P<0.05）。综上,大狼毒2种主要化感物质的添加增加了山地草甸土壤微生物物种数目,促进了土壤微生物的生长繁殖,但不同物质对土壤微生物多样性的影响存在差异,与4-硝基苯酚相比,2-苯乙胺的影响程度更高。

关键词: 大狼毒, 化感物质添加, 土壤微生物, 优势菌相对丰度, 物种多样性

CLC Number:

S812.6

DUAN Rui, LUO Qin, XIAO Xue, NIU Qiong-mei, LIU Yang, YANG Xue, LU Jia-qi, CHU Xiao-hui, SHAN Gui-lian. Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow[J]. Acta Agrestia Sinica, 2025, 33(1): 10-20.

段蕊, 罗钦, 肖雪, 牛琼梅, 刘洋, 杨雪, 陆嘉琪, 初晓辉, 单贵莲. 大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响[J]. 草地学报, 2025, 33(1): 10-20.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2025.01.002

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2025/V33/I1/10

References

[1] CHEN Z W,ZHAO D S,ZHU Y,et al. Response of grassland soil respiration to experimental warming：The long-term effects may be greater than we thought[J]. Soil Biology & Biochemistry,2022,168：108616
[2] ZHAO X,SONG Y Q,XU T T,et al. Edge effects and spatial degradation process in highly fragmented grassland-impact on soil microbial community[J]. Ecological Indicators,2021,132：108307
[3] 谢勇,尹海燕,初晓辉,等. 放牧和封育对滇西北亚高山草甸土壤养分及微生物特性的影响[J]. 云南农业大学学报（自然科学）,2017,32（6）：1121-1128
[4] 王猛,袁福锦,吴文荣,等. 封育对滇西北亚高山草甸群落及土壤的影响[J]. 草原与草坪,2017,37（2）：69-74
[5] GUO L Z,LI J H,HE W,et al. High nutrient uptake efficiency and high water use efficiency facilitate the spread of Stellera chamaejasme L. in degraded grasslands[J]. BMC Ecology,2019,19（1）：50
[6] 席赠濮,杜凡,汪健. 香格里拉县亚高山退化草甸植物群落学研究[J]. 西南林业大学学报,2013,33（2）：75-80
[7] 李嘉懿,初晓辉,尹海燕,等. 大狼毒型亚高山草甸植被演替对围封的响应[J]. 中国草地学报,2021,43（6）：10-16
[8] FENG A Q,LI Y Z,GAO J B,et al. The determinants of streamflow variability and variation in Three-River Source of China：climate change or ecological restoration?[J]. Environmental Earth Sciences,2017,76（20）：696
[9] 张静,温仲明,李鸣雷,等. 外来物种刺槐对土壤微生物功能多样性的影响[J]. 生态学报,2018,38（14）：4964-4974
[10] 马建国,侯扶江,BOWATTE S. 青藏高原高寒草甸有毒植物对土壤理化性质和土壤微生物丰度的影响[J]. 草业科学,2019,36（12）：3033-3040
[11] 程济南. 瑞香狼毒（Stellera chamaejasme L.）根际微环境对其种群扩张的影响研究[D]. 兰州：兰州大学,2022：4-9
[12] 王占青,张杰雪,杨雪莲,等. 高寒草甸不同斑块草地土壤微生物多样性特征研究[J]. 草地学报,2021,29（9）：1916-1926
[13] 王玉琴,宋梅玲,周睿,等. 黄帚橐吾对退化高寒草地植物、土壤养分及微生物量的影响[J]. 草业学报,2022,31（12）：31-40
[14] 朱珣之,李强,李扬苹,等. 紫茎泽兰入侵对土壤细菌的群落组成和多样性的影响[J]. 生物多样性,2015,23（5）：665-672
[15] CHEN B M,GAO Y,LIAO H X,et al. Differential responses of invasive and native plants to warming with simulated changes in diurnal temperature ranges[J]. AoB Plants,2017,9（4）：plx028
[16] 陈宝明,韦慧杰,陈伟彬,等. 外来入侵植物对土壤氮转化主要过程及相关微生物的影响[J]. 植物生态学报,2018,42（11）：1071-1081
[17] 祁小旭,张思宇,林峰,等. 黄顶菊对不同入侵地植物群落及土壤微生物群落的影响[J]. 生态学报,2019,39（22）：8472-8482
[18] GUO G F, LI H,SHI Y,et al. Continental-scale plant invasions reshuffle the soil microbiome of blue carbon ecosystems[J]. Global Change Biology,2022,28（14）：4423-4438
[19] ZHAO Y H,MEMMOTT J,VAUGHAN I P,et al. The impact of a native dominant plant, Euphorbia jolkinii, on plant-flower visitor networks and pollen deposition on stigmas of co-flowering species in subalpine meadows of Shangri-La, SW China[J]. Journal of Ecology,2021,109（5）：2107-2120
[20] 罗钦,马祖艳,牛琼梅,等. 大狼毒不同部位浸提液对多年生黑麦草幼苗生长的化感效应及生理机制[J]. 草地学报,2023,31（10）：3212-3219
[21] 段蕊,罗钦,肖雪,等. 大狼毒次生代谢物分析及主要物质化感效应研究[J]. 草地学报,2024,32（8）：2599-2606
[22] 牛琼梅,单贵莲,罗钦,等. 毒害草入侵扩散对滇西北亚高山草甸土壤微生物多样性的影响[J]. 草地学报,2023,31（7）：1996-2004
[23] 初晓辉,谢勇,单贵莲,等. 放牧和封育对青藏高原南缘亚高山草甸群落结构及物种多样性的影响[J]. 草地学报,2017,25（5）：939-945
[24] 高雪峰,韩国栋. 短花针茅根系分泌物对荒漠草原土壤细菌群落及土壤养分的影响[J]. 中国草地学报,2021,43（6）：76-84
[25] WARDLE D A,BARDGETT R D,KLIRONOMOS J N,et al. Ecological linkages between aboveground and belowground biota[J]. Science,2004,304（5677）：1629-1633
[26] BOSSIO D A,SCOW K M. Impacts of carbon and flooding on soil microbial communities： Phospholipid fatty acid profiles and substrate utilization patterns[J]. Microbial Ecology,1998,35（3）：265-278
[27] WU Y P,MA B,ZHOU L,et al. Changes in the soil microbial community structure with latitude in Eastern China, based on phospholipid fatty acid analysis[J]. Applied Soil Ecology,2009,43（2/3）：234-240
[28] HUANG X M,LIU S R,WANG H,et al. Changes of soil microbial biomass carbon and community composition through mixing nitrogen-fixing species with Eucalyptus urophylla in subtropical China[J]. Soil Biology and Biochemistry,2014,73：42-48
[29] COBAN O,DE DEYN G B,VAN DER PLOEG M. Soil microbiota as game-changers in restoration of degraded lands[J]. Science,2022,375（6584）：abe0725
[30] 全小龙,陈梦词,史慧兰,等. 毒杂草降解对土壤微生物多样性的影响[J]. 西南农业学报,2024,37（4）：844-851
[31] STEFANOWICZ A M,KAPUSTA P,STANEK M,et al. Invasive plant Reynoutria japonica produces large amounts of phenolic compounds and reduces the biomass but not activity of soil microbial communities[J]. Science of the Total Environment,2021,767：145439
[32] ZHANG P,LI B,WU J H,et al. Invasive plants differentially affect soil biota through litter and rhizosphere pathways： a meta-analysis[J]. Ecology Letters,2019,22（1）：200-210
[33] GAGGINI L,RUSTERHOLZ H P,BAUR B. The invasive plant Impatiens glandulifera affects soil fungal diversity and the bacterial community in forests[J]. Applied Soil Ecology,2018,124：335-343
[34] ZHALNINA K,LOUIE K B,HAO Z,et al. Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly[J]. Nature Microbiology,2018,3：470-480
[35] CHENG J N,JIN H,ZHANG J L,et al. Effects of allelochemicals, soil enzyme activities, and environmental factors on rhizosphere soil microbial community of Stellera chamaejasme L. along a growth-coverage gradient[J]. Microorganisms,2022,10（1）：158
[36] YANG S S,SUN J Y,LIU C,et al. Stellera chamaejasme expansion promotes the restoration of soil microbial diversity and ecosystem multifunctionality in degraded grasslands[J].Catena,2024,241：108020
[37] YU H X,LE ROUX J J,ZHAO M X,et al. Mikania sesquiterpene lactones enhance soil bacterial diversity and fungal and bacterial activities[J]. Biological Invasions,2023,25（1）：237-250
[38] LI C G,DENG Y,WANG J G,et al. Effects of p-Hydroxyphenylacetic acid and p-Hydroxybenzoic acid on soil bacterial and fungal communities[J]. Sustainability,2023,15（12）：9285
[39] CHE R X,LIU D,QIN J L,et al. Increased litter input significantly changed the total and active microbial communities in degraded grassland soils[J]. Journal of Soils and Sediments,2020,20（7）：2804-2816
[40] 李海云,姚拓,马亚春,等. 祁连山中段退化高寒草地土壤细菌群落分布特征[J]. 草业学报,2019,28（8）：170-179
[41] 单贵莲,尹海燕,刘洋,等. 大狼毒种群扩散增殖对滇西北亚高山草甸土壤养分及微生物特性的影响[J]. 云南农业大学学报（自然科学）,2021,36（3）：494-499
[42] FANG K,WANG Y Z,ZHANG H B. Differential effects of plant growth-promoting bacteria on invasive and native plants[J]. South African Journal of Botany,2019,124：94-101
[43] ZHAO M X,LU X F,ZHAO H X,et al. Ageratina adenophora invasions are associated with microbially mediated differences in biogeochemical cycles[J]. Science of the Total Environment,2019,677：47-56

Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow

大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DUAN Rui, LUO Qin, XIAO Xue, NIU Qiong-mei, LIU Yang, GUI Bao-lin, ZHONG Li-qiong, CHU Xiao-hui, SHAN Gui-lian. Analysis and Study of Secondary Metabolites and Allelopathic Effects of Main Substances of Euphorbia jolkinii [J]. Acta Agrestia Sinica, 2024, 32(8): 2599-2606.
[2]	QIAO Qian-luo, WU Yan-ru, LI Qing-yao, ZHANG Yang-can, KOU Wei-liang, HE Xin-ting, LI Xi-lai, KOU Jian-cun, YANG Wen-quan. The Effects of Commercial Organic Fertilizer and Semi Decomposed Sheep Manure on Artificial Grassland and Soil Characteristics in Alpine Mining Area [J]. Acta Agrestia Sinica, 2024, 32(8): 2659-2669.
[3]	WANG Zi-han, LYU Shi-jie, WANG Zhong-wu, WU Lan-ao-deng, LIU Hong-mei, MA Sheng-yun, LI Zhi-guo, HAN Guo-dong. The Relationship between Species Diversity and Aboveground Standing Crop of Plant Community in the Desert Steppe [J]. Acta Agrestia Sinica, 2024, 32(6): 1856-1863.
[4]	DENG Tong-tong, ZHOU Guo-ying, XIAO Yuan-ming, JIN Yu-ting, LI Chang-bin. Effects of Long-term Precipitation Changes and Nitrogen Addition on Species Diversity and Productivity of Alpine Grasslands in the Qinghai Tibet Plateau [J]. Acta Agrestia Sinica, 2024, 32(5): 1448-1458.
[5]	WANG Jing-jie, ZHU Jian-hang, GAO Xin, HAN Ming-yu, REN De-zhi, WAN Dan. Analysis of Plant Species Diversity and Interspecific Connectivity in Typical Wetlands in The Middle and Lower Reaches of Lhasa River [J]. Acta Agrestia Sinica, 2024, 32(3): 918-927.
[6]	ZHANG Xiao-jia, ZHU Yi, WU Qian, JU Xin, SHEN Ting-ting, HAN Guo-dong. Effects of Stocking Rates on Phylogenetic Diversity of Plant Communities in Desert Steppe [J]. Acta Agrestia Sinica, 2024, 32(10): 3289-3296.
[7]	TUO Hang-hang, HUANG Jian-di, BAI Yue, TIAN Hui-hui, LI Mei-hui, WANG Zi-lin, YANG Meng-ru, WANG Yi-bo, YE Fa-ming, LI Wei. Characteristics of Soil Nitrogen Mineralization and its Influencing Factors before and after Growing Season in Subshrub Expansion Grassland [J]. Acta Agrestia Sinica, 2023, 31(9): 2730-2739.
[8]	WANG Jun-wei, MING Sheng-ping, XU Min, LA Qiong. Diversity Pattern and Phylogenetic Structure of Plant Communities in Alpine Ecological Key Zone [J]. Acta Agrestia Sinica, 2023, 31(9): 2777-2786.
[9]	NIU Qiong-mei, SHAN Gui-lian, LUO Qin, LIU Yang, LIU Jia, XIE Yong, DENG Shen-cai, YANG Xin, CHU Xiao-hui. Effect of Invasion and Diffusion of Poisonous Weeds on Soil Microbial Diversity in Subalpine Meadow in Northwest Yunnan [J]. Acta Agrestia Sinica, 2023, 31(7): 1996-2004.
[10]	SUN Ya-fei, CHAI Yong-qing. Species Diversity of Gymnocarpos przewalskii Community in Different Habitats and its Relationship with the Soil Moisture and Salinity in the Western End of Qilian Mountain [J]. Acta Agrestia Sinica, 2023, 31(7): 2059-2067.
[11]	ZHOU Hua-kun, LI Shan, SUN Jian, QU Jia-peng, ZHANG Zhong-hua, MA Li, QIN Rui-min, WEI Jing-jing, CHANG Tao, SU Hong-ye, HU Xue, A Di-ha-ze, YUAN Fang, LI Hong-lin. Characteristics of Plant Community and Soil Physical and Chemical Properties in Alpine Meadow along Altitude Gradient in the Headwaters Region of Three-River on Tibetan Plateau [J]. Acta Agrestia Sinica, 2023, 31(6): 1735-1743.
[12]	WANG Ju, XU Ming-xiang, SUN Hui, ZUO Zhen-jiang, AI Jian-wei. Effects of Grazing on Community Characteristics of Robinia pseudoacacia Forest in the Hilly Loess Plateau Region of Northern Shaanxi Province [J]. Acta Agrestia Sinica, 2023, 31(6): 1826-1833.
[13]	WEI Jia-qi, ZHENG Cheng, CUI Meng-ying, SHEN Qiao-tian, JIN Xiang-qian, WEN Zhong-ming. Analysis on the Relationship between Biodiversity and Ecosystem Function in Loess Hilly Region [J]. Acta Agrestia Sinica, 2023, 31(5): 1490-1500.
[14]	WANG Li-jun, WU Jia-qi, ALIMIRI-Alimujiang, ZHAO Yi-fan, CUI Wen-kai, KONG Fan-xi, CHEN Jun. The Contribution of Different Plant Species to the Species Diversity of Grassland Community [J]. Acta Agrestia Sinica, 2023, 31(4): 1081-1088.
[15]	WEN Bo-jin, DUAN Gao-hui, WEN Zhong-ming. Coupling Effects of Biodiversity with Topographic and Soil Factors on Slope Runoff-Sediment Yield [J]. Acta Agrestia Sinica, 2023, 31(4): 1186-1197.