The Effects of Invasion Plant Parthenium hysterophorus on Soil Enzyme Activity and Microbial Nutrient Limitation

doi:10.11733/j.issn.1007-0435.2025.01.009

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (1): 70-78.DOI: 10.11733/j.issn.1007-0435.2025.01.009

The Effects of Invasion Plant Parthenium hysterophorus on Soil Enzyme Activity and Microbial Nutrient Limitation

WANG Han-zhi¹, MA Xiao-ming¹, FANG Xue-mei¹, YIN Rui¹, WANG Wen-long¹, DENG Xin-xin², ZHANG Lin³, DONG Xian-yong², Lamu Nima^1,4, LIU Lin¹

1. Grassland Science and Technology College, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China;
2. China Three Gorges Construction Engineering Corporation, Beijing 101100, China;
3. Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan Province 610041, China;
4. Gêrzê County Bureau of Agriculture and Rural, Affairs of Agriculture and Animal Husbandry Technology Extension Station, Ali, Xizang 859200, China

Received:2024-04-23 Revised:2024-06-05 Published:2025-01-22

入侵植物银胶菊对土壤酶活性及微生物养分限制的影响

王汉之¹, 马小明¹, 方雪梅¹, 殷瑞¹, 王文龙¹, 邓鑫欣², 张林³, 董先勇², 尼玛拉姆^1,4, 刘琳¹

1. 四川农业大学草业科技学院, 四川成都 611130;
2. 中国三峡建工(集团)有限公司, 北京 101100;
3. 中国科学院山地生态恢复与生物资源利用重点实验室/生态恢复与生物多样性保育四川省重点实验室/中国科学院成都生物研究所, 四川成都 610041;
4. 西藏阿里地区改则县农业农村局农牧业技术推广站, 西藏阿里 859200

通讯作者: 刘琳,E-mail：liulinsky@126.com
作者简介:王汉之（1999-）,男,汉族,甘肃兰州人,硕士研究生,主要从事入侵生态学研究,E-mail：2197065545@qq.com
基金资助:
中国三峡（建工）集团有限公司科研项目（JG-EP-030222001,JG-EP-030222002）资助

Abstract

Abstract: To explore the effects and mechanisms of plant invasion on soil enzyme activity and microbial nutrient limitations,this study focused on the invasive plant Parthenium hysterophorus in the Wu Dongde Reservoir area along the Jinsha River,using the native plant Cynodon dactylon as a control. The study analyzed the differences in soil physicochemical properties,extracellular enzyme activities,and microbial nutrient limitations in the rhizosphere and non-rhizosphere soils of both plants. The results showed that the rhizosphere soil of P. hysterophorus exhibited significantly higher alkaline phosphatase and β-1,4-glucosidase activities,as well as higher available phosphorus content compared to C. dactylon,while β-1,4-N-acetylglucosaminidase activity showed the opposite trend. Additionally,the vector angle and length in both the rhizosphere and non-rhizosphere soils of P. hysterophorus were significantly higher than those of C. dactylon,indicating that soil microbes in P. hysterophorus were more severely limited by both carbon and phosphorus. In summary,the invasion of P. hysterophorus significantly enhanced the hydrolytic enzyme activities related to carbon and phosphorus in the rhizosphere soil and exacerbated the carbon and phosphorus limitations of soil microbes in both the rhizosphere and non-rhizosphere soils.

Key words: Invasion plant, Enzyme stoichiometric ratio, Rhizosphere, Nutrient cycle, Jinsha River

摘要： 为探究植物入侵对土壤酶活性及微生物营养限制的影响及其机制,本研究以金沙江乌东德电站库区入侵植物银胶菊（Parthenium hysterophorus）为研究对象,乡土植物狗牙根（Cynodon dactylon）为对照,分析它们根围（根际和非根际）土壤理化性质、胞外酶活性和微生物养分限制的差异。结果显示：银胶菊根际土碱性磷酸酶（Alkaline phosphatase,AP）和β-1,4-葡萄糖苷酶（β-Glucosidase,BG）活性及速效磷养分含量显著高于狗牙根,而β-1,4-N-乙酰氨基葡萄糖苷酶（N-Acetylglucosidase,NAG）则相反（P<0.05）；银胶菊根际和非根际矢量角度（>45°）和长度均显著高于狗牙根（P<0.05）,表明银胶菊土壤微生物受碳和磷的共同限制,且限制程度均高于狗牙根。综上,银胶菊入侵使根际土壤碳磷水解酶活性明显提升,且显著加剧了根际非根际土壤微生物碳、磷限制。

关键词: 入侵植物, 酶化学计量学, 根际, 养分循环, 金沙江

CLC Number:

Q948.113

WANG Han-zhi, MA Xiao-ming, FANG Xue-mei, YIN Rui, WANG Wen-long, DENG Xin-xin, ZHANG Lin, DONG Xian-yong, Lamu Nima, LIU Lin. The Effects of Invasion Plant Parthenium hysterophorus on Soil Enzyme Activity and Microbial Nutrient Limitation[J]. Acta Agrestia Sinica, 2025, 33(1): 70-78.

王汉之, 马小明, 方雪梅, 殷瑞, 王文龙, 邓鑫欣, 张林, 董先勇, 尼玛拉姆, 刘琳. 入侵植物银胶菊对土壤酶活性及微生物养分限制的影响[J]. 草地学报, 2025, 33(1): 70-78.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2025.01.009

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2025/V33/I1/70

References

[1] SIMBERLOFF D,MARTIN J L,GENOVESI P,et al. Impacts of biological invasions：what’s what and the way forward[J]. Trends in Ecology & Evolution,2013,28（1）：58-66
[2] CARBONI M,LIVINGSTONE S W,ISAAC M E,et al. Invasion drives plant diversity loss through competition and ecosystem modification[J]. Journal of Ecology,2021,109（10）：3587-3601
[3] LEE M R,BERNHARDT E S,VAN BODEGOM P M,et al. Invasive species’ leaf traits and dissimilarity from natives shape their impact on nitrogen cycling：a meta-analysis[J]. New Phytologist,2017,213（1）：128-139
[4] ZHANG H Y,GONCALVES P,COPELAND E,et al. Invasion by the weed Conyza canadensis alters soil nutrient supply and shifts microbiota structure[J]. Soil Biology and Biochemistry,2020,143：107739
[5] XU H W,LIU Q,WANG S Y,et al. A global meta-analysis of the impacts of exotic plant species invasion on plant diversity and soil properties[J]. Science of the Total Environment,2022,810：152286
[6] OJONG M P J,ALVAREZ M,IHLI H J,et al. Action on invasive species：control strategies of Parthenium hysterophorus L. on smallholder farms in Kenya[J]. Environmental Management,2022,69（5）：861-870
[7] BOJA M,GIRMA Z,DALLE G. Impacts of Parthenium hysterophorus L. on plant species diversity in ginir district,southeastern ethiopia[J]. Diversity,2022,14（8）：675
[8] SAFDAR M E,TANVEER A,KHALIQ A,et al. Critical competition period of Parthenium weed （Parthenium hysterophorus L.） in maize[J]. Crop Protection,2016,80：101-107
[9] ZHANG P,LI B,WU J,et al. Invasive plants differentially affect soil biota through litter and rhizosphere pathways：a meta-analysis[J]. Ecology Letters,2019,22（1）：200-210
[10] QIN Z,XIE J F,QUAN G M,et al. Impacts of the invasive annual herb Ambrosia artemisiifolia L. on soil microbial carbon source utilization and enzymatic activities[J]. European Journal of Soil Biology,2014,60： 58-66
[11] CHAPIN F S III,MATSON P A,MOONEY H A. Principles of terrestrial ecosystem ecology[M]. New York： Springer,2002： 436
[12] SINSABAUGH R L,LAUBER C L,WEINTRAUB M N,et al. Stoichiometry of soil enzyme activity at global scale [J]. Ecology Letters,2008,11（11）：1252-1264
[13] ALLISON S D,VITOUSEK P M. Responses of extracellular enzymes to simple and complex nutrient inputs[J]. Soil Biology and Biochemistry,2005,37（5）：937-944
[14] SINSABAUGH R L,HILL B H,FOLLSTAD SHAH J J. Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment[J]. Nature,2009,462（7274）：795-798
[15] ALLISON S D,NIELSEN C,HUGHES R F. Elevated enzyme activities in soils under the invasive nitrogen-fixing tree Falcataria moluccana[J]. Soil Biology and Biochemistry,2006,38（7）：1537-1544
[16] HU Z Y,LI J T,SHI K,et al. Effects of Canada goldenrod invasion on soil extracellular enzyme activities and ecoenzymatic stoichiometry[J]. Sustainability,2021,13（7）：3768
[17] 邓鑫欣,于江,熊浩荣,等. 金沙江下游乌东德和白鹤滩电站库区外来入侵植物组成和分布[J]. 应用与环境生物学报,2023,29（6）：1305-1321
[18] MAO R,NGUYEN T L T,OSUNKOYA O O,et al. Spread pathways of the invasive weed Parthenium hysterophorus L.：the potential for water dispersal[J]. Austral Ecology,2019,44（7）：1111-1122
[19] NIGATU L,HASSEN A,SHARMA J,et al. Impact of Parthenium hysterophorus on grazing land communities in north-eastern Ethiopia[J]. Weed Biology and Management,2010,10（3）：143-152
[20] 熊浩荣,邓鑫欣,于江,等. 金沙江下游外来入侵植物入侵风险评估——以乌东德和白鹤滩电站库区为例[J].生物安全学报（中英文）,2024,33（4）：381-382
[21] COWIE B W,BYRNE M J,WITKOWSKI E T F. Small-scale insights into the above- and below-ground invasion dynamics of Parthenium hysterophorus in a South African savanna：the potential role of stocking rate[J]. South African Journal of Botany,2022,144：229-237
[22] PUTRI W E. Allelopathic effect of Parthenium weed （Parthenium hysterophorus L.） leaf litter on the seedling emergence and growth of wheat （Triticum aestivum L.）[J].IOP Conference Series：Earth and Environmental Science,2022,978（1）：012016
[23] 陈新微,李慧燕,刘红梅,等. 入侵种银胶菊和三叶鬼针草与本地种气体交换特性的比较[J]. 生态学报,2016,36（18）：5732-5740
[24] LUSTER J,GÖTTLEIN A,NOWACK B,et al. Sampling,defining,characterising and modeling the rhizosphere—the soil science tool box[J]. Plant and Soil,2009,321（1-2）：457-482
[25] 王雪梅,闫帮国,赵广,等. 云南元谋不同海拔土壤微生物对车桑子碳、氮、磷化学计量特征及土壤特性的影响[J]. 植物生态学报,2017,41（3）：311-324
[26] RILEY D,BARBER S A. Bicarbonate accumulation and pH changes at the soybean （Glycine max （L.） merr.） root-soil interface[J]. Soil Science Society of America Journal,1969,33（6）：905-908
[27] JENKINSON D S, BROOKES P C,POWLSON D S. Measuring soil microbial biomass[J]. Soil Biology and Biochemistry,2004,36（1）：5-7
[28] MOORHEAD D L,SINSABAUGH R L,HILL B H,et al. Vector analysis of ecoenzyme activities reveal constraints on coupled C,N and P dynamics[J]. Soil Biology and Biochemistry,2016,93：1-7
[29] 李雅男,李邵宇,史世斌,等. 荒漠草原不同放牧强度下土壤酶化学计量特征的研究[J].草地学报,2022,30（9）：2239-2248
[30] ZHANG P,NIE M,LI B,et al. The transfer and allocation of newly fixed C by invasive Spartina alterniflora and native Phragmites australis to soil microbiota[J]. Soil Biology and Biochemistry,2017,113：231-239
[31] 李会娜,刘万学,万方浩,等. 入侵植物豚草与本地植物马唐对土壤肥力与酶活性影响的比较[J]. 中国生态农业学报,2009,17（5）：847-850
[32] 李伟华,张崇邦,林洁筠,等. 外来入侵植物的氮代谢及其土壤氮特征[J]. 热带亚热带植物学报,2008,16（4）：321-327
[33] 潘森,卜嘉玮,甘安琪,等. 放牧强度对高寒草地土壤微生物胞外酶化学计量的影响[J]. 草地学报,2023,31（6）：1780-1787
[34] 闫静,张晓亚,陈雪,等. 三叶鬼针草与不同本地植物竞争对土壤微生物和土壤养分的影响[J]. 生物多样性,2016,24（12）：1381-1389
[35] 张燕平,赵粉侠,刘秀贤,等. 干热河谷印楝生长与立地条件关系[J]. 林业科学研究,2005,18（1）：74-79
[36] WANG T,ZHU B,ZHOU M H,et al. Nutrient loss from slope cropland to water in the riparian zone of the Three Gorges Reservoir：process,pathway,and flux[J]. Agriculture,Ecosystems & Environment,2020,302： 107108
[37] 唐赛春,韦春强,潘玉梅,等. 入侵植物银胶菊对不同氮、磷水平的繁殖适应性[J]. 武汉植物学研究,2010,28（2）： 213-217
[38] XU H W,QU Q,CHEN Y H,et al. Disentangling the direct and indirect effects of cropland abandonment on soil microbial activity in grassland soil at different depths[J]. Catena,2020,194：104774
[39] EHRENFELD J G,KOURTEV P,HUANG W. Changes in soil functions following invasions of exotic understory plants in deciduous forests[J]. Ecological Applications,2001,11（5）：1287-1300
[40] HAICHAR F E Z,HEULIN T,GUYONNET J P,et al. Stable isotope probing of carbon flow in the plant holobiont[J].Current Opinion in Biotechnology,2016,41：9-13
[41] KAUR A,KAUR S,SINGH H P,et al. Alterations in phytotoxicity and allelochemistry in response to intraspecific variation in Parthenium hysterophorus[J]. Ecological Complexity,2022,50：100999
[42] TAMURA M,SUSEELA V. Warming and labile substrate addition alter enzyme activities and composition of soil organic carbon[J]. Frontiers in Forests and Global Change,2021,4：691302
[43] YANG T,LI X,HU B,et al. Climate and soil properties shape latitudinal patterns of soil extracellular enzyme activity and stoichiometry：Evidence from Southwest China[J]. Applied Soil Ecology,2024,197：105319
[44] LI W H,ZHANG C B,JIANG H B,et al. Changes in soil microbial community associated with invasion of the exotic weed,Mikania micrantha H.B.K[J]. Plant and Soil,2006,281（1）：309-324
[45] DOGRA K S,SOOD S K,SHARMA R. Distribution, Biology and Ecology of Parthenium hysterophorus L. （Congress Grass） an invasive species in the North-Western Indian Himalaya （Himachal Pradesh）[J]. African Journal of Plant Science,2011,5（11）：682-687
[46] TIAN L,SHI W. Short-term effects of plant litter on the dynamics,amount,and stoichiometry of soil enzyme activity in agroecosystems[J]. European Journal of Soil Biology,2014,65：23-29
[47] SONG Y Y,SONG C C,TAO B X,et al. Short-term responses of soil enzyme activities and carbon mineralization to added nitrogen and litter in a freshwater marsh of Northeast China[J]. European Journal of Soil Biology,2014,61：72-79
[48] HERNÁNDEZ D L,HOBBIE S E. The effects of substrate composition,quantity,and diversity on microbial activity[J]. Plant and Soil,2010,335（1）：397-411
[49] SPOHN M,KUZYAKOV Y. Phosphorus mineralization can be driven by microbial need for carbon[J]. Soil Biology and Biochemistry,2013,61：69-75
[50] DENG L,PENG C H,HUANG C B,et al. Drivers of soil microbial metabolic limitation changes along a vegetation restoration gradient on the Loess Plateau,China[J]. Geoderma,2019,353：188-200
[51] CUI Y,FANG L,GUO X,et al. Natural grassland as the optimal pattern of vegetation restoration in arid and semi-arid regions：Evidence from nutrient limitation of soil microbes[J]. Science of the Total Environment,2019,648：388-397
[52] SI C C,LIU X Y,WANG C Y,et al. Different degrees of plant invasion significantly affect the richness of the soil fungal community[J]. Plos One,2013,8（12）：e85490
[53] 王亚男,李睿玉,朱晓换,等. 土荆芥挥发油化感胁迫对土壤胞外酶活性和微生物多样性的影响[J]. 生态学报,2017,37（13）：4318-4326
[54] LIU C H,WANG B R,ZHU Y Z,et al. Eco-enzymatic stoichiometry and microbial non-homeostatic regulation depend on relative resource availability during litter decomposition[J]. Ecological Indicators,2022,145： 109729
[55] 陆宇明,许恩兰,吴东梅,等. 凋落物双倍添加和移除对米槠林土壤水解酶活性及其化学计量比的影响[J]. 水土保持学报,2021,35（4）：313-320
[56] 李慧超,潘红丽,冯秋红,等. 凋落叶输入量对川西亚高山不同密度云杉人工林土壤酶活性及其化学计量比的影响[J]. 2024,43（10）：2967-2978
[57] YANG Y,LIANG C,WANG Y Q,et al. Soil extracellular enzyme stoichiometry reflects the shift from P- to N-limitation of microorganisms with grassland restoration[J]. Soil Biology and Biochemistry,2020,149：107928

The Effects of Invasion Plant Parthenium hysterophorus on Soil Enzyme Activity and Microbial Nutrient Limitation

入侵植物银胶菊对土壤酶活性及微生物养分限制的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Lu, LIU Jia-xin, LI Si-yu, CHEN Ning, BAO Feng-xuan, WANG Feng, QU Shan-min, LIU Guo-fu. Effects of Saline-Alkali Tolerant Rhizosphere Growth-Promoting Bacteria on the Growth and Root Physiological Characteristics of Alfalfa under Saline-Alkali Stress [J]. Acta Agrestia Sinica, 2024, 32(7): 2323-2329.
[2]	WANG Rui, LIU Hai-li, ZHU Ai-min, WANG Yu-xin, REN Zi-huan, HAN Guo-dong. Effects of Stocking Rates on Soil Bacteria and Carbon and Nitrogen Content of Artemisia frigida at Different Rhizosphere Distances [J]. Acta Agrestia Sinica, 2024, 32(2): 386-395.
[3]	WANG Xue-ying, BAO Xin-guang, ZHANG Feng, TAN Bing-bing, WANG Yu-ying, CHONG Pei-fang. Characteristics of Bacterial Community and Soil Enzyme Activity in Rhizosphere Soil of Desert Plant Reaumuria Soongorica [J]. Acta Agrestia Sinica, 2024, 32(12): 3764-3773.
[4]	ZHANG Xin-yue, SUI Yong-chao, WANG Qiong, YANG Yue-qing, LI Jin-bo, LI Chang-yi, XU Li-xin. Analysis of Fungal Community Structure and Diversity in Rhizosphere Soil of Native Plants in Dexing Mining Area [J]. Acta Agrestia Sinica, 2024, 32(10): 3103-3112.
[5]	LIANG Peng-fei, LI Jing-feng, NAN Li-li, GUO Quan-en, CAO Shi-yu. Response of the Structure of Bacterial Community in the Rhizosphere of Medicago sativa 'Qingshui' to Copper and Nickel Stress [J]. Acta Agrestia Sinica, 2023, 31(7): 2170-2176.
[6]	LU Wen-jie, WU Cong, QI Jin-yun, JING Ya-hong. Effects of the Co-application of Biochar and Inoculants on the Alfalfa Biomass and Soil Characteristics [J]. Acta Agrestia Sinica, 2023, 31(5): 1578-1587.
[7]	HUANG Chen, HAN Ling-juan, LIANG Yin-ping, YANG Kai-yuan, JIANG Lin, SUN Xiao-han, FAN Le, ZHAO Xiang, GAO Peng. Identification and Plant Growth Promotion Analysis of Four Salt-alkali Tolerant Rhizosphere-promoting Bacteria Isolated from Lespedeza daurica [J]. Acta Agrestia Sinica, 2023, 31(4): 1036-1047.
[8]	HUANG Chen, YANG Kai-yuan, GAO Peng, LIANG Yin-ping, HAN Ling-juan, ZHAO Xiang. Screening, Identification and Characteristics of Phosphate-Solubilizing Microorganisms in Lespedeza daurica [J]. Acta Agrestia Sinica, 2022, 30(9): 2345-2355.
[9]	ZHAO Fang-cao, CHEN Hong-fei, WANG Yi-hao, DONG Kuan-hu, WANG Chang-hui, CHEN Xiao-peng. Response of Rhizosphere Soil Properties to Changed Precipitation and Nitrogen Addition in a Salinized Grassland [J]. Acta Agrestia Sinica, 2022, 30(9): 2430-2437.
[10]	FU Li-jiao, LI Xue-qin, FAN Ji-hui, LU Xu-yang, YAN Yan. Analysis of Rhizosphere Soil Bacterial Community Structure Diversity and Root Characteristics of Typical Plants in Alpine Steppe of Northern Tibet [J]. Acta Agrestia Sinica, 2022, 30(5): 1131-1140.
[11]	ZAN Kan-zhuo, Ashanjiang·Yiminijiang, LIU Cong, TAN Kai, BAI Jie, HE Shu-bin, WANG Ke-zhen, QU Yang, LONG Ming-xiu. Physicochemical Properties and Stoichiometric Characteristics of Rhizosphere and Bulk Soils of Alfalfa of Different Fall Dormancy Types [J]. Acta Agrestia Sinica, 2022, 30(4): 957-965.
[12]	MA Kun, LU Guang-xin, DENG Ye, YAN Hui-lin, WANG Ying-cheng, ZHAO Yang-an, ZHANG Hai-juan, ZHOU Xue-li, DOU Sheng-yun. Effects of Applying Organic Fertilizer on Rhizosphere Microorganisms and Soil Physical and Chemical Properties of Elymus nutans [J]. Acta Agrestia Sinica, 2022, 30(3): 594-602.
[13]	LI Sheng-yi, SUN Yan-liang, LIU Xuan-shuai, ZHAO Jun-wei, ZHAO Jian-tao, MA Chun-hui, ZHANG Qian-bing. Effects of Phosphate Fertilizer on Rhizosphere Soil Microenvironment and Hay Yield of Alfalfa under Drip Irrigation [J]. Acta Agrestia Sinica, 2022, 30(2): 495-502.
[14]	SU Jing-Long, QI Juan, LIU Yan-Jun, YANG Hang, LI Ming. Effects of Different Nitrogen Levels and Nitrogen Application Methods on Rhizosphere Soil Characteristics of Elymus sibiricus L. [J]. Acta Agrestia Sinica, 2021, 29(2): 259-269.
[15]	ZHU Rui-fen, LIU Jie-lin, WANG Jian-li, HAN Wei-bo, SHEN Zhong-bao, LI Da. Study on Bacterial Community Diversity and Environmental Factors in Rhizosphere Soil of Leymuschinensis [J]. Acta Agrestia Sinica, 2020, 28(3): 652-660.