Effects of Lactiplantibacillus plantarum AS98 on Alfalfa Silage Quality, Microorganisms and Phytol Content

doi:10.11733/j.issn.1007-0435.2025.10.035

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3477-3484.DOI: 10.11733/j.issn.1007-0435.2025.10.035

Effects of Lactiplantibacillus plantarum AS98 on Alfalfa Silage Quality, Microorganisms and Phytol Content

MU Yuan-yuan¹, KONG De-hui¹, CAO Yang^1,2,3, ZANG Yan-qing⁴

1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Heilongjiang Provincial Key Laboratory of Efficient Utilization of Feed Resources and Nutrition Manipulation in Cold Region, Daqing, Heilongjiang Province 163319, China;
2. Engineering Research Center of Processing and Utilization of Grain By-products, Ministry of Education, Daqing, Heilongjiang Province 163319, China;
3. Key Laboratory of Green and Low-carbon Agriculture in the Northeastern China Plain, Ministry of Agriculture and Rural Affairs P. R. China, Daqing, Heilongjiang Province 163319, China;
4. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, China

Received:2025-01-20 Revised:2025-03-02 Published:2025-10-17

植物乳杆菌AS98对苜蓿青贮品质、微生物及叶绿醇含量的影响

穆园园¹, 孔德慧¹, 曹阳^1,2,3, 臧延青⁴

1. 黑龙江八一农垦大学动物科技学院/黑龙江省寒区饲料资源高效利用与营养调控重点实验室, 黑龙江大庆 163319;
2. 粮食副产物加工与利用教育部工程研究中心, 黑龙江大庆 163319;
3. 农业农村部东北平原农业绿色低碳重点实验室, 黑龙江大庆 163319;
4. 黑龙江八一农垦大学食品学院, 黑龙江大庆 163319

通讯作者: 曹阳,E-mail:hbdkcaoyang@163.com;臧延青,E-mail:byndzangyanqing@163.com
作者简介:曹阳,E-mail:hbdkcaoyang@163.com;臧延青,E-mail:byndzangyanqing@163.com
基金资助:
国家自然科学基金项目（No.32171676）；黑龙江省双一流特色学科平台项目（HLJ2022TSXK）资助

Abstract

Abstract: To investigate the effects of AS98 （Lactiplantibacillus plantarum）， which was isolated from silage in the earlier stage， on the quality and phytol content of alfalfa （Medicago sativa L.） silage， a single^-factor experimental design was adopted. The treatments included the control group （CK）， the AS98 group （1×10⁷ cfu·mL^-1 L. plantarum AS98 strain， L. plantarum）， the LP group （1×10⁷ cfu·mL^-1 commercial bacterium Lacticaseibacillus paracasei， LP） and the AS98+LP group （（0.5 + 0.5）×10⁷ cfu·mL^-1 Lactiplantibacillus plantarum AS98 + commercial bacterium LP compound bacteria）. The results showed that in comparison with the control group， all the additive-added groups enhanced the sensory quality of alfalfa silage. The AS98 group significantly reduced the contents of neutral detergent fiber and acid detergent fiber， and significantly increased the contents of crude protein， crude fat， and phytol （P<0.05）. Compared to the control group， all additive-added groups significantly increased the lactic acid content （P<0.05）. Additionally， the quantities of lactic acid bacteria in the AS98 group and the AS98+LP group were significantly higher than those in the control group （P<0.05）， while the pH value and the content of ammonium-nitrogen were significantly lower than those in the control group （P<0.05）， and the numbers of aerobic bacteria， bacillus， and yeast were significantly decreased （P<0.05）， and the numbers of Aerobic Bacteria， Bacillus and Yeast decreased significantly （P<0.05）. In conclusion， the addition of AS98 can improve the fermentation quality of alfalfa silage and significantly increase phytol content. This study provides a theoretical basis and functional strains for AS98 as an additive for the production of alfalfa silage enriched with phytol.

Key words: Alfalfa silage, Lactiplantibacillus, Microbial population, Fermentation quality, Phytol

摘要： 为了研究前期从青贮中分离的植物乳杆菌（Lactiplantibacillus plantarum）AS98对苜蓿（Medicago sativa L.）青贮品质及叶绿醇含量的影响，试验采取单因素设计，处理包括对照组、AS98组（添加1×10⁷ cfu·mL^-1植物乳杆菌AS98菌株）、LP组（添加1×10⁷ cfu·mL^-1商品菌（Lacticaseibacillus paracasei， LP）和AS98+LP组（0.5×10⁷ cfu·mL^-1植物乳杆菌AS98和0.5×10⁷ cfu·mL^-1商品菌LP复合菌）。结果表明，与对照组比，添加组均改善了苜蓿青贮的感官品质，AS98组对减少中性洗涤纤维与酸性洗涤纤维含量以及提高粗蛋白、粗脂肪以及叶绿醇含量作用显著（P<0.05）；所有添加组与对照组相比显著提高了乳酸含量（P<0.05），并且AS98组和AS98+LP组乳酸菌数量含量显著高于对照组（P<0.05），pH值和氨态氮含量显著低于对照组（P<0.05），好氧性细菌、芽孢杆菌、酵母菌数量显著降低（P<0.05）。综上所述，添加AS98可以改善紫花苜蓿青贮发酵品质，显著提高叶绿醇含量，本研究为AS98作为生产富集叶绿醇的苜蓿青贮添加剂提供了理论基础和功能菌株。

关键词: 苜蓿青贮, 乳酸菌, 微生物组成, 发酵品质, 叶绿醇

CLC Number:

S816.53

MU Yuan-yuan, KONG De-hui, CAO Yang, ZANG Yan-qing. Effects of Lactiplantibacillus plantarum AS98 on Alfalfa Silage Quality, Microorganisms and Phytol Content[J]. Acta Agrestia Sinica, 2025, 33(10): 3477-3484.

穆园园, 孔德慧, 曹阳, 臧延青. 植物乳杆菌AS98对苜蓿青贮品质、微生物及叶绿醇含量的影响[J]. 草地学报, 2025, 33(10): 3477-3484.

References

[1] LAZARIDI E，KAPAZOGLOU A，GERAKARI M，et al. Crop Landraces and indigenous varieties: a valuable source of genes for plant breeding[J]. Plants，2024，13（6）:2223-7747
[2] SUN H，KANG X，TAN H， et al. Progress in fermented unconventional feed application in monogastric animal production in China[J]. Fermentation，2023，9（11）:947-948
[3] 酆炳森，刘玉珊，刘凌霄，等. 乳酸菌与纤维素酶对丹参地上部分青贮品质的影响[J]. 草地学报，2024，32（10）:3313-3319
[4] 张雪，郭宗明，李英，等. 叶绿素皂化反应条件的优化研究[J]. 生物化工，2017，3（5）:60-62
[5] RONALD R J，KOMEN J，FERDINANDUSSE S. Phytanic acid metabolism in health and disease[J]. Biochimica et Biophysica Acta （BBA）-Molecular and Cell Biology of Lipids，2011，1811（9）:498-507
[6] GRIMALDI P A. Peroxisome Proliferator-Activated Receptors as sensors of fatty acids and derivatives[J]. Cellular and Molecular Life Sciences，2007（19-20）:2459-2464
[7] 中华人民共和国农业农村部.NY/T 4673-2025，首蓿青贮质量评定综合指数法[S].北京:中国农业出版社，2025:5-7
[8] WANG C，HE L W，XING Y Q，et al. Fermentation quality and microbial community of Alfalfa and Stylo Silage mixed with Moringa oleifera leaves[J]. Bioresource Technology，2019:284（7）:240-247
[9] 冯宗慈，高民. 通过比色测定瘤胃液氨氮含量方法的改进[J]. 畜牧与饲料科学，2010，31（6）:37
[10] AOAC. Official methods of analysis[M]. Arlington，VA:As-sociation of Official Analytical Chemists，1990，15（8）:33-35
[11] THOMAS C. Book review: the biochemistry of Silage[J]. Outlook on Agriculture，1991，20（4）:272
[12] CALO-MATA P， ARLINDO S， BOEHME K， et al. Current applications and future trends of Lactic Acid Bacteria and their Bacteriocins for the biopreservation of aquatic food products[J]. Food and Bioprocess Technology，2008（1）:43-63
[13] 晋秀娟，孙丽丽，赵锴，等. 小麦叶绿素酶基因家族的鉴定及其叶绿素降解过程中的功能预测[J]. 激光生物学报，2022，31（1）:50-60
[14] LIU Y， WANG Y， ZHANG L， et al. The effect of Lactobacillus planturum YQM48 inoculation on the quality and microbial community structure of Alfalfa Silage cultured in Saline-Alkali Soil[J]. Fermentation，2023，9（6）:511
[15] KALAČ P. The effects of Silage feeding on some sensory and health attributes of cow’s milk: A review[J]. Food Chemistry，2011，125（2）:307-317
[16] MEZGEBO G B， MONAHAN F J， MCGEE M， et al. Fatty Acid， volatile and sensory characteristics of beef as affected by Grass Silage or Pasture in the Bovine diet-ScienceDirect[J]. Food Chemistry，2017，235:86-97
[17] 赵雅茹，许庆方，高文俊，等. 抑霉乳酸菌脱毒特性及青贮应用的研究[J]. 生物技术通报，2021，37（9）:95-105
[18] PAHLOW G，MUCK R E，DRIEHUIS F，et al. Microbiology of Ensiling[J]， Advances in Applied Microbiology，2003，53:1-73
[19] ÁVILA C L，CARVALHO B F，PINTO J C，et al. The use of Lactobacillus species as starter cultures for enhancing the quality of Sugar Cane Silage[J]. Journal of Dairy Science，2014，97（2）:940-951
[20] ELLIS J L，HINDRICHSEN I K，KLOP G，et al. Effects of Lactic Acid Bacteria Silage Inoculation on Methane Emission and productivity of Holstein Friesian dairy cattle[J]. Journal of Dairy Science，2016，99（9）:7159-7174
[21] 储徐建. 耐低温乳酸菌的筛选与六种原料青贮中的应用[D]. 西宁:青海大学，2014:38-53
[22] 陈勇辉，韩乐，钱玲亚，等. 植物乳杆菌对不同含水量多花黑麦草青贮品质的影响[J]. 江西畜牧兽医杂志，2023（4）:41-44
[23] LANGSTON C W，WISEMAN H G，GORDON C H，et al. Chemical and bacteriological changes in Grass Silage during the early stages of Fermentation I. Chemical Changes[J]. Journal of Dairy Science，1962，45（3）:396-402
[24] CHAUHAN N，KUMARI N，MANI V，et al. Effects of Lactiplantibacillus plantarum， Limosilactobacillus fermentum， and Propionic Acid on the fermentation process of Sugarcane tops Silages along with variations in pH， Yeast and Mould count after aerobic exposure[J]. Waste and Biomass Valorization，2024，15（4）:2215-2230
[25] 闫威明，陈雅坤，杨鹏标，等. 青贮饲料质量评定方法研究进展[J]. 中国畜牧兽医，2024，51（1）:135-144
[26] CHEN F，WANG J，ZHANG S，et al. Aerobic stability， in vitro digestibility， and rumen degradation characteristics of Silages mixed with Sweet Sorghum and aerial parts of Licorice[J]. Agriculture （Basel），2024，14（2）:212
[27] KUNG L JR，SHAVER R D，et al. Silage review: Interpretation of Chemical， Microbial， and Organoleptic components of silages[J]. Journal of Dairy Science，2018，101（5）:4020-4033
[28] KUNG L，RANJIT N K. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of Barley Silage[J]. Journal of Dairy Science，2001，84:1149-1155
[29] MUCK R. Revista brasileira de zootecnia Silage Microbiology and its control through additives[J]. Journal of Animal Science-REV BRAS ZOOTECN，2010，39:183
[30] ERTEKIN I，KIZILSIMSEK M. Effects of Lactic Acid Bacteria inoculation in pre-harvesting period on fermentation and feed quality properties of Alfalfa Silage[J]. Asian-Australasian Journal of Animal Sciences，2020，33（2）:217-224
[31] 杨智明，王琴，曲善民，等. 苜蓿青贮过程中三种酶活性动态研究[J]. 中国草地学报，2012，34（1）:113-116
[32] 吕仁龙，胡海超，张兴波，等. 不同刈割高度王草中叶绿素和叶绿醇含量在青贮前后的变动[J]. 动物营养学，2019，31（9）:4208-4217
[33] 陈秉龙. 发酵条件和青贮时间对黑麦草色素和植物醇含量的影响[J]. 中国饲料，2020 （10）:25-28
[34] VERHOEVEN N M，WANDERS R J，POLL-THE B T，et al. The metabolism of Phytanic Acid and Pristanic Acid in man: a review[J]. Journal of Inherited Metabolic Disease，1998，21（7）:697-728
[35] LV R，BITSU MABROUK O，TAKETO SUGINO，et al. Effect of varying fermentation conditions with ensiling period and inoculum on Photosynthetic Pigments and Phytol content in Italian Ryegrass （Lolium multiflorum Lam.） silage[J]. Animal Science Journal，2020（1）:91
[36] 孔德慧，靳思玉，曹阳. 乳酸菌和混合酸对苜蓿青贮品质、叶绿素及其降解酶的影响[J]. 草地学报，2024，32（5）:1610-1618
[37] SÁENZ J S，RIOS-GALICIA B，REHKUGLER B，et al. Dynamic Development of Viral and Bacterial Diversity during Grass Silage Preservation[J]. Viruses，2023，15（4）:924
[38] 李苗苗，靳思玉，王立超，等. 不同温度下添加乳酸菌对油莎草青贮品质及体外干物质消失率的影响[J]. 动物营养学报，2020，32（2）:827-835
[39] 塔娜，魏日华，德庆哈拉，等. 对禾草源同型发酵和/或异型发酵乳酸菌发酵无芒雀麦青贮有氧稳定性的评价[J]. 动物营养学报，2017，29（4）:1301-1311
[40] 曹阳，臧延青，黄秋连，等. 一株提高苜蓿青贮中植物醇含量的菌株及其应用:中国，CN202210657226.3[P]. 2022-09-06

Effects of Lactiplantibacillus plantarum AS98 on Alfalfa Silage Quality, Microorganisms and Phytol Content

植物乳杆菌AS98对苜蓿青贮品质、微生物及叶绿醇含量的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZOU Yu-qi, WANG Zhi-jun, LIU Li-ying, YUAN Ye, PAN Ze-yu, MENG He-gaole, BAO Le-er, GE Gen-tu. Effects of Spirulina Peptides on Fermentation Quality and Microbial Community of Alfalfa [J]. Acta Agrestia Sinica, 2025, 33(8): 2685-2693.
[2]	CHEN Hao, GAO Wan-shun, WANG Xu-zhe, MA Chun-hui. Nutrients and Fermentation Quality of Different Varieties of Sesbania cannabina and Zea mays [J]. Acta Agrestia Sinica, 2025, 33(8): 2713-2720.
[3]	YAN Xu, HOU Xing-feng, WANG Hong-lin, ZUO Yan-chun, XIAO Lian, WU Zi-zhou, LI Yang, CHEN Hui, LIU Hong-mei, SUN Ru-ting, YANG Jian, ZHOU Hong-yan, LIU Jing-song, ZHANG Qiong-wen, DU Zhou-he. Quality Analysis of Silage in Agricultural Region of Sichuan Province [J]. Acta Agrestia Sinica, 2025, 33(7): 2051-2058.
[4]	SHI Ya-qi, FAN Wen-na, SHI Peng-fei, ZHANG Meng-yao, YANG Yi-xin. Effect of Forsythia Suspensa Addition on the Quality of Alfalfa Silage [J]. Acta Agrestia Sinica, 2025, 33(7): 2400-2406.
[5]	SONG Jia, WEI Lu-heng, HU Yi-fei, WANG Yi-bo, WANG Dong, SUN Juan-juan, XUE Yan-lin, BAI Chun-sheng, YU Zhu. Effect of Storage Temperature and Bacillus Addition on Fermentation Quality and Nitrate Content of Sorghum-sudangrass Silage [J]. Acta Agrestia Sinica, 2025, 33(5): 1669-1676.
[6]	CHEN Gui-wu, NONG Zhi-yan, CHEN Na, LUO Shi-yong, SHI Xiao-lu, LI Ying-han, ZI Xue-juan, LI Mao. Effects of Different Proportions of Piper sarmentosum on the Fermentation Quality and In Vitro Fermentation Characteristics of King Grass Silage [J]. Acta Agrestia Sinica, 2025, 33(5): 1677-1685.
[7]	REN Jia-hui, ZHANG Teng-wei, BAO Wen-long, YUN Ying, Wang Zhao-lan, LIU Zhi-ping, ZHENG Cheng-zhong, Wang Feng-wu, Wang Jing-yu, Yang Feng-ting, QIAO Gong, QU Jia-hui, LI Tao, SUN Juan-juan. Effects of Planting Density on the Quality of Whole Barley Silage [J]. Acta Agrestia Sinica, 2025, 33(2): 630-635.
[8]	GAO Yu, YAO Kang-yu, ZHAI Mei-yan, ABUDULI Subinuer, SONG Lin-hai, YANG Min, SHAO Wei, YANG Liang, REN Wan-ping. Effects of Different Moisture Contents and Compaction Densities on the Nutritional Composition,Fermentation Quality,and Microbial Diversity of High-moisture Corn Grain Silage [J]. Acta Agrestia Sinica, 2025, 33(10): 3455-3468.
[9]	MA Tian-li, LI Xiao-mei, ZHONG Yi-hao, LUO Hong-jie, DU Zhao-chang, XIAO Qi-yin, LIU Wei-guo, YAN Yan-hong. Study on the Silage Quality of Mixed Whole Plant Maize and Different Legume Forage in the Northwest Plateau Area of Sichuan Region [J]. Acta Agrestia Sinica, 2024, 32(9): 2999-3005.
[10]	XU Gui, WANG Ya-ting, MU Lin, WANG Qing-lan, WEI Lan, GUO Yang, WEI Zhong-shan, ZHANG Zhi-fei. Meta-Analysis Study of the Effects of Adding Urea on Silage Quality [J]. Acta Agrestia Sinica, 2024, 32(8): 2607-2619.
[11]	CHAI Yu-xin, JIA Shu-an, ZHANG Tian, LI Xiao, CHEN Yong-cheng, HUANG Rong-zheng, ZHANG Fan-fan. Effects of Lactiplantibacillus plantarum on Silage Quality of Urtica cannabina and Rumen Degradation Characteristics of Sheep [J]. Acta Agrestia Sinica, 2024, 32(7): 2283-2289.
[12]	WU Qi-feng, CHEN Chen, HUANG Qin-jiao, ZHANG Yun-fei, LI Xiao-mei, CHEN Yuan-hang, WEN Xing-jin, YAN Yan-hong. A Effects of Cellulase and High-temperature Tolerant Lactic Acid Bacteria on Structural Carbohydrate of (Pennisetum ameri-canum×P.purpureum)×P.durpureum schum. [J]. Acta Agrestia Sinica, 2024, 32(7): 2314-2322.
[13]	CHENG Zhi-ze, YISILAYI-Dawuti, WAN Jiang-chun, NAIBI-Abulaiti, AIBIBULA-Yimamu. Effects of Different Additives on the Quality and Bacterial Diversity of Pepper Straw Silage [J]. Acta Agrestia Sinica, 2024, 32(7): 2330-2336.
[14]	HEI Meng-qin, CAO Zheng-yu, WANG Zhi-yuan, DAI Chun-hui, CHEN Cheng, ZHANG Wen-ju. Effects of Adding Compound Lactobacillus and Molasses on the Quality of Alfalfa Silage with Different Moisture Contents [J]. Acta Agrestia Sinica, 2024, 32(5): 1601-1609.
[15]	KONG De-hui, JIN Si-yu, CAO Yang. Effects of Lactic Acid Bacteria and Mixed Acids on Fermentation Quality,Chlorophyll and Chlorophyll Degrading Enzymes in Alfalfa Silage [J]. Acta Agrestia Sinica, 2024, 32(5): 1610-1618.