Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3827-3836.DOI: 10.11733/j.issn.1007-0435.2025.11.033

Previous Articles    

The Alleviating Effects of CaCl2 and 24-EBL on Chicory Seedlings under Pb and Cd Stress

YUAN Xin-yue1, LIU Yu-heng1, YU Bao-gang1,2, QIN Xiang-yu1,2, LI Tie1,2, LIU Li-jie1,2, JIN Zhong-min1,2, HAO Yu3   

  1. 1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
    2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Biodiversity Conservation in Cold Regions, Qiqihar, Heilongjiang Province 161006, China;
    3. Hainan Geological Comprehensive Survey and Design Institute, Haikou, Hainan Province 570206, China
  • Received:2025-07-03 Revised:2025-08-26 Published:2025-11-13

Pb、Cd胁迫下CaCl2与24-EBL对菊苣幼苗的缓解效应

苑新悦1, 刘宇恒1, 于保刚1,2, 秦祥宇1,2, 李铁1,2, 刘丽杰1,2, 金忠民1,2, 郝宇3   

  1. 1. 齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006;
    2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006;
    3. 海南地质综合勘察设计院, 海南 海口 570206
  • 通讯作者: 金忠民,E-mail:yyy6768@163.com;郝宇,E-mail:1955471218@qq.com
  • 作者简介:苑新悦（1999-），女，汉族，硕士研究生，黑龙江绥化人，主要从事植物逆境生理与分子生物学研究，E-mail：15145212511@163.com
  • 基金资助:
    黑龙江省省属高等学校基本科研业务费植物性食品专项（NO.145309523）资助

PDF

Abstract: To investigate efficient and environmentally friendly strategies for mitigating lead （Pb） and cadmium （Cd） stress in plants， this study integrated calcium chloride （CaCl2） and 24-Epibrassinolide （24-EBL） to establish a combined intervention system based on “calcium signal regulation plus plant hormone regulation”. Chicory （Cichorium intybus L.） subjected to Pb and Cd stress was expected to exhibit enhanced tolerance through the synergistic effects of this dual regulation. The experiment optimized three treatment combinations： Treatment B （CaCl2 5 mmol·L-1 + 24-EBL 0.06 mg·L-1）， Treatment C （CaCl2 10 mmol·L-1 + 24-EBL 0.04 mg·L-1）， and Treatment D （CaCl2 10 mmol·L-1 + 24-EBL 0.06 mg·L-1）. Under varying levels of Pb and Cd stress， chicory plants were subjected to Treatments B， C， and D， respectively， and their physiological parameters were subsequently analyzed. The results showed that the three treatments varied in their effectiveness in reducing Pb and Cd accumulation across different plant tissues and in enhancing stress resistance. Grey relational analysis demonstrated that Treatment B （CaCl2 5 mmol·L-1 + 24-EBL 0.06 mg·L-1） was the most effective in alleviating Pb- and Cd-induced oxidative damage in chicory seedlings. It significantly reduced Pb and Cd concentrations in chicory， enhanced the non-enzymatic antioxidant system， and promoted polyphenol synthesis.

Key words: Chicory, 24-EBL, CaCl2, Heavy metal stress

摘要： 为探寻缓解植物铅（Pb）、镉（Cd）胁迫的高效、绿色方法，本研究将氯化钙（CaCl2）与24-表油菜素内酯（24-Epibrassinolide， 24-EBL）协同施用，构建“钙信号调节+植物激素调控”的复合干预体系，并应用于Pb、Cd胁迫下的菊苣（Cichorium intybus L.），期望通过二者协同效应，增强其抗性。试验优化出3种处理组合：B（CaCl2 5 mmol·L-1+24-EBL 0.06 mg·L-1）、C（CaCl2 10 mmol·L-1+24-EBL 0.04 mg·L-1）、D（CaCl2 10 mmol·L-1+24-EBL 0.06 mg·L-1）。在不同Pb、Cd胁迫条件下，分别用B、C、D组处理菊苣并测定其生理指标。结果表明，B、C、D组在降低菊苣各部分Pb、Cd含量及提高Pb、Cd抗性方面表现出差异化。灰色关联分析发现，B组（CaCl2 5 mmol·L-1+24-EBL 0.06 mg·L-1）在缓解Pb、Cd对菊苣幼苗造成的氧化损伤、降低菊苣体内Pb、Cd含量、促进非酶抗氧化物循环及多酚合成方面效果最佳。

关键词: 菊苣, 24-EBL, CaCl2, 重金属胁迫

CLC Number: