Effects of NaCl Stress on Proline Accumulation and Metabolic Pathways of Chicory(Cichorium intybus L.) Seedlings

doi:10.11733/j.issn.1007-0435.2011.01.018

›› 2011, Vol. 19 ›› Issue (1): 102-106.DOI: 10.11733/j.issn.1007-0435.2011.01.018

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Effects of NaCl Stress on Proline Accumulation and Metabolic Pathways of Chicory(Cichorium intybus L.) Seedlings

WANG Kang^1,2, LIU Yan-xiang¹, DONG Jie³, ZHOU He², DONG Kuan-hu¹

1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Institute of Grassland Science, China Agriculture University, Beijing 100193, China;
3. Institute of animal science, CAAS, Beijing 100193, China

Received:2010-06-30 Revised:2010-12-06 Online:2011-02-15 Published:2011-02-15

盐胁迫对菊苣幼苗脯氨酸积累及其代谢途径的影响

王康^1,2, 刘艳香¹, 董洁³, 周禾², 董宽虎¹

1. 山西农业大学动物科技学院, 太符, 山西, 030801;
2. 中国农业大学动物科技学院草地研究所, 北京, 100193;
3. 中国农业科学院北京畜牧兽医研究所, 北京, 100193

通讯作者: 董宽虎,E-mail:dongkuanhu@sxau.edu.cn
作者简介:王康(1982- ),男,山西霍州人,博士研究生,研究方向为植物抗逆生理,E-mail:wangkang1982@yahoo.com.cn
基金资助:
“十一五”国家科技支撑计划课题“退化草地植被恢复与重建关键技术研究”(2007BAD₅6B01)资助

Abstract

Abstract: Proline plays important roles in regulation of osmotic homeostasis,prevention of damages caused by osmotic stresses,scavenging of reactive oxygen species and protection of cell structures.Many plants accumulate proline dramatically under a variety of stress conditions.The regulation of proline accumulation in Chicory seedlings and the activities of key enzymes involved in proline metabolism in response to salinity were studied here.Fifty-day-old seedlings were treated with NaCl(0,100,200,300 and 400 mM) in this experiment.Results show that Ornithine-oxo-acid transaminase(δ-OAT) activity increases;Pyrroline-5-carboxylate synthetase(P5CS) activity initially increases then deccreases,while ProDH activity decreases with increasing NaCl concentration.Proline contents increase significantly(P<0.05) with increasing salt-stress followed by a declining response during the extending salt-stress period.The data demonstrate that glutamate and ornthine pathways are activated by NaCl.The glutamate pathway is predominant under low-concentration NaCl treatments,and an ornithine pathway is predominant under high-concentration NaCl treatments.

Key words: Chicory(Cichorium intybus L.), Proline metabolism, Salt stress, δ-OAT, P5CS, ProDH

摘要： 以菊苣(Cichorium intybus L.)为材料,研究不同浓度NaCl处理对菊苣幼苗体内脯氨酸代谢的影响,以期揭示菊苣中脯氨酸代谢的基本规律。结果表明:随着处理浓度的增加,菊苣中鸟氨酸δ-氨基转移酶(δ-OAT)活性呈上升趋势;吡咯啉-5-羧酸合成酶(P5CS)活性呈现先升后降的趋势;脯氨酸脱氢酶(ProDH)活性呈下降趋势;而脯氨酸含量则显著上升(P<0.05),但高浓度长时间处理后略有降低。研究表明,NaCl胁迫下谷氨酸(Glu)途径和鸟氨酸(Orn)途径对菊苣中脯氨酸的积累均有贡献,低浓度胁迫时P5CS起关键作用的Glu途径占主导地位,高浓度胁迫时δ-OAT起关键作用的Orn途径占主导地位。

关键词: 菊苣, 脯氨酸代谢, 盐胁迫, 鸟氨酸δ-氨基转移酶, 吡咯啉-5-羧酸合成酶, 脯氨酸脱氢酶

CLC Number:

Q945.78

WANG Kang, LIU Yan-xiang, DONG Jie, ZHOU He, DONG Kuan-hu. Effects of NaCl Stress on Proline Accumulation and Metabolic Pathways of Chicory(Cichorium intybus L.) Seedlings[J]. , 2011, 19(1): 102-106.

王康, 刘艳香, 董洁, 周禾, 董宽虎. 盐胁迫对菊苣幼苗脯氨酸积累及其代谢途径的影响[J]. , 2011, 19(1): 102-106.

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Effects of NaCl Stress on Proline Accumulation and Metabolic Pathways of Chicory(Cichorium intybus L.) Seedlings

盐胁迫对菊苣幼苗脯氨酸积累及其代谢途径的影响

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