Molecular Mechanism of RBP47C Regulating Alfalfa Growth

doi:10.11733/j.issn.1007-0435.2025.02.008

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (2): 401-409.DOI: 10.11733/j.issn.1007-0435.2025.02.008

Molecular Mechanism of RBP47C Regulating Alfalfa Growth

DU Hong-qi¹, YAN Xiang-zhou¹, FENG Chang-song¹, HAN Kang-kang², LYU Xian-zhao², LOU Zhi-guo¹, LIU Lei¹, LIANG Zhi-yan¹

1. Institute of Animal Husbandry, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province 450002, China;
2. Henan Huahuaniu Industrial Group Co., Ltd., Zhengzhou, Henan Province 450002, China

Received:2024-04-12 Revised:2024-06-11 Published:2025-03-01

RBP47C调控苜蓿生长的分子机理研究

杜红旗¹, 闫祥洲¹, 冯长松¹, 韩康康², 吕先召², 娄治国¹, 刘磊¹, 梁志妍¹

1. 河南省农业科学院畜牧研究所, 河南郑州 450002;
2. 河南花花牛实业集团有限公司, 河南郑州 450002

通讯作者: 冯长松,E-mail:fengchangsong72@163.com
作者简介:杜红旗（1981-）,男,汉族,河南睢县人,博士,助理研究员,主要从事牧草分子生理研究和育种工作,E-mail:biodhq@126.com
基金资助:
河南省农业科学院新兴学科发展专项项目（2024XK07）;河南省农业科学院自主创新项目（2024ZC094）;河南省科技攻关计划项目（202102110084）资助

Abstract

Abstract: In order to clarify the molecular mechanism of RBP47C regulating of alfalfa （Medicago sativa L.） growth, this study analyzed the relative mRNA expression of RBP47C-silenced plants under different day lengths or different temperatures, observed the phenotype changes of RBP47C-silenced plants, the transcriptome of RBP47C-silenced plants and control plants was analyzed by high-throughput sequencing to screen their significant difference genes, RBP47C protein binding genes were screened by RIP- seq. The results indicate that the expression of RBP47C gene was negatively regulated by light time and positively regulated by temperature. The growth of RBP47C-silenced alfalfa plants were accelerated in spring and inhibited in summer； The dry weight of RBP47C-silenced alfalfa plants increased and the ratio of leaf to stem decreased. A total of 2629 genes with significant difference between the apical buds of RBP47C-silenced alfalfa plants and wild plants were obtained, and 66 possible RBP47C protein-binding genes were obtained by RIPseq. There are 20 common differential genes between the both. The conlusion is that the expression of RBP47C gene is regulated by light time and temperature, and is mainly negatively regulated by light time in autumn. We got 15 genes that are probably RBP47C protein binding genes, and RBP47C protein regulates growth and stress resistance of alfalfa by regulating the metabolic pathways involved in these genes. The growth of RBP47C-silenced alfalfa plants was accelerated and the yield was increased under suitable conditions, but their stress resistance was weaker, the ratio of leaf to stem was decreased and the quality was slightly poorer.

Key words: RBP47C, Alfalfa, Regulation of gene expression, Growth, Stress resistance

摘要： 为阐明RBP47C调控苜蓿（Medicago sativa L.）生长的分子机理,本研究通过检测分析不同光照时间或不同温度下其mRNA相对表达量,观察RBP47C沉默苜蓿植株与对照植株的表型变化,高通量测序分析RBP47C沉默植株与对照植株顶芽转录组,筛选二者显著差异基因,对苜蓿顶芽采用RIP-seq筛选RBP47C可能结合基因,最后通过比对获得二者共有的基因。结果表明：RBP47C表达受光照时间负调控、受温度正调控；RBP47C沉默植株在春季生长加快,在夏季生长受到抑制；RBP47C沉默植株总干重增加、叶茎比降低；获得RBP47C沉默植株与对照植株顶芽中显著差异基因2629个,得到66个RBP47C结合的可能基因,二者共有基因20个。RBP47C表达同时受光照时间和温度的调控,且在秋季主要受光照时间负调控；RBP47C沉默植株在适宜条件下加速生长,提高了产量,但其抗逆性变弱、叶茎比降低；得到RBP47C很可能结合的15个基因,RBP47C通过调节这些基因参与的代谢途径调控苜蓿的生长和抗逆性。

关键词: RBP47C, 苜蓿, 基因表达调控, 生长, 抗逆性

CLC Number:

S541

DU Hong-qi, YAN Xiang-zhou, FENG Chang-song, HAN Kang-kang, LYU Xian-zhao, LOU Zhi-guo, LIU Lei, LIANG Zhi-yan. Molecular Mechanism of RBP47C Regulating Alfalfa Growth[J]. Acta Agrestia Sinica, 2025, 33(2): 401-409.

杜红旗, 闫祥洲, 冯长松, 韩康康, 吕先召, 娄治国, 刘磊, 梁志妍. RBP47C调控苜蓿生长的分子机理研究[J]. 草地学报, 2025, 33(2): 401-409.

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Molecular Mechanism of RBP47C Regulating Alfalfa Growth

RBP47C调控苜蓿生长的分子机理研究

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