Abstract: In order to investigate the effects of sheep slat manure application on soil physicochemical properties, microbial community structure and its function in alpine mining area, this study set different sheep slat manure application rates （0 m³·m^-2, CK； 0.04 m³·m^-2, T1； 0.05 m³·m^-2, T2； 0.06 m³·m^-2, T3）, and measured and analyzed the soil-related parameters in the second year after application. The results showed that the application of sheep slat manure significantly increased soil total nitrogen, total phosphorus, soil organic matter, quick-acting nitrogen and quick-acting phosphorus contents, and significantly changed the Beta diversity of bacterial and fungal communities, the relative abundance of some dominant microflora and functional microorganisms. Redundancy analysis revealed that soil organic matter, total nitrogen and total phosphorus were the key soil physicochemical factors affecting the distribution of soil bacterial and fungal communities in alpine mining areas. In summary, the application of sheep slat manure had obvious promotion effects on soil quality and microbial environment in alpine mining areas, and it suggested that the dosage of sheep slat manure should be set to 0.06 m³·m^-2 for soil remediation in the Muli mining area.

Key words: Alpine mining area, Sheep slat manure, Soil physicochemical properties, Microbial community structure, Redundancy analysis

摘要： 为探究施用羊板粪对高寒矿区土壤理化性质、微生物群落结构及其功能的影响,本研究设置了不同羊板粪施用量（0 m³·m^-2,CK；0.04 m³·m^-2,T1；0.05 m³·m^-2,T2；0.06 m³·m^-2,T3）,对施用后第二年的土壤相关参数进行了测定和分析,发现施用羊板粪显著提高了土壤全氮、全磷、土壤有机质、速效氮和速效磷含量,并显著改变了细菌和真菌群落的Beta多样性、部分优势微生物菌群及功能微生物的相对丰度。冗余分析发现,土壤有机质、全氮和全磷是影响高寒矿区土壤细菌和真菌群落分布的关键土壤理化因子。综合而言,施用羊板粪对高寒矿区的土壤质量和微生物环境具有促进作用,建议木里矿区土壤修复可将羊板粪用量设置为0.06 m³·m^-2。

关键词: 高寒矿区, 羊板粪, 土壤理化性质, 微生物群落结构, 冗余分析