Effects of S-ABA on Photosynthesis Characteristic of Elymus nutans Under Combined Low Temperature and Drought Stresses

doi:10.11733/j.issn.1007-0435.2025.05.008

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (5): 1418-1424.DOI: 10.11733/j.issn.1007-0435.2025.05.008

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Effects of S-ABA on Photosynthesis Characteristic of Elymus nutans Under Combined Low Temperature and Drought Stresses

YU Yi-ze¹, WANG Rui-jia¹, LONG Si¹, RAN Shu-qi¹, LI Han-yu¹, YANG Jin-huan², XU Yue-fei¹

1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shannxi Province 712100, China;
2. Haiyuan County Forestry and Grassland Bureau, Zhongwei, Ningxia 755200, China

Received:2024-08-09 Revised:2024-09-30 Online:2025-05-15 Published:2025-05-20

S-诱抗素对低温干旱复合胁迫下垂穗披碱草光合特征的影响

余易泽¹, 王芮嘉¹, 龙思¹, 冉姝琪¹, 李晗玉¹, 杨金环², 许岳飞¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 海原县林业和草原局, 宁夏中卫 755200

通讯作者: 许岳飞,E-mail:xuyuefei@nwafu.edu.cn
作者简介:余易泽（1999-），男，汉族，重庆永川人，硕士研究生，主要从事牧草、草坪草逆境生物学研究，E-mail：yuyize@nwafu.edu.cn；王芮嘉（1998-），女，汉族，四川眉山人，硕士研究生，主要从事牧草、草坪草逆境生物学研究，E-mail：wmdwrj@nwafu.edu.cn；龙思（1998-），女，汉族，陕西汉中人，博士研究生，主要从事牧草、草坪草逆境生物学研究，E-mail：long1998@nwafu.edu.cn
基金资助:
科技部“十四五”国家重点研发计划子课题（No.2023YFD1301401-2）；陕西省重点研发计划-重点产业链项目（No. 2021ZDLNY02-04）资助

Abstract

Abstract: In order to investigate the effects of S-ABA （S-abscisic acid） on the photosynthesis characteristic of Elymus nutansunder combined low temperature and drought stresses. By using wild Elymus nutans as the study material， we investigated the effects of exogenous spraying of S-ABA and endogenous ABA inhibitor （Fluridone） on plant morphology， photosynthetic pigments， gas exchange parameters， chlorophyll fluorescence parameters， and stomatal state of Elymus nutans under combined low temperature and drought stresses， with the aim of providing a theoretical basis for abiotic stress study of Elymus nutans. The results showed that the combined low temperature and drought stresses significantly decreased the content of photosynthetic pigments and photosynthetic capacity of Elymus nutans. The application of S-ABA significantly increased P_n， G_s， T_r， F_v/F_m， and ETR，and decreased C_i and NPQ. Additionally， S-ABA mitigated the effects of stress on leaf stomatal conductance， improved plant morphology， and promoted increases in plant height， leaf width， and relative water content， while Fluridone application attenuated these positive effects. In summary， S-ABA effectively mitigates the adverse effects of combined low temperature and drought stresses on the photosynthesis of Elymus nutans， providing crucial guidance for improving forage production efficiency in arid and cold regions.

Key words: S-ABA, Combined stress, Elymus nutans, Photosynthesis characteristic

摘要： 为探究S-诱抗素（S-abscisic acid，S-ABA）对低温干旱复合胁迫下垂穗披碱草（Elymus nutans）光合特征的影响，以野生垂穗披碱草为研究材料，研究外源喷施S-ABA和内源ABA抑制剂（Fluridone）对低温干旱复合胁迫下垂穗披碱草形态、光合色素、光合参数、荧光参数和气孔状态的影响，旨在为垂穗披碱草非生物胁迫研究提供理论依据。结果表明，低温干旱复合胁迫显著降低了垂穗披碱草的光合色素含量和光合作用能力。喷施S-ABA显著增加了净光合速率（P_n）、蒸腾速率（P_s）、气孔导度（T_r）、最大光能转化效率（F_v/F_m）和电子传递速率，降低了胞间CO₂浓度（C_i）和非光化猝灭系数。此外，S-ABA还缓解了胁迫对叶片气孔状态的影响，改善了植物形态，促进株高、叶宽和相对含水量的增加，而Fluridone的应用减弱了这些积极效果。综上，S-ABA能够有效缓解低温干旱复合胁迫对垂穗披碱草光合作用的不利影响，这对于提高干旱寒冷地区牧草的生产效率具有重要指导意义。

关键词: S-诱抗素, 复合胁迫, 垂穗披碱草, 光合特征

CLC Number:

S543.9

YU Yi-ze, WANG Rui-jia, LONG Si, RAN Shu-qi, LI Han-yu, YANG Jin-huan, XU Yue-fei. Effects of S-ABA on Photosynthesis Characteristic of Elymus nutans Under Combined Low Temperature and Drought Stresses[J]. Acta Agrestia Sinica, 2025, 33(5): 1418-1424.

余易泽, 王芮嘉, 龙思, 冉姝琪, 李晗玉, 杨金环, 许岳飞. S-诱抗素对低温干旱复合胁迫下垂穗披碱草光合特征的影响[J]. 草地学报, 2025, 33(5): 1418-1424.

References

[1] STIRBET A，LAZÁR D，GUO Y，et al. Photosynthesis： basics， history and modelling[J]. Annals of Botany，2020，126（4）：511-537
[2] 饶德民，董青松，程彤，等. 作物光合效率对逆境适应及提高途径研究进展[J]. 植物生理学报，2024，60（8）：1229-1240
[3] 张政，刘美君，王婕，等. 低温下紫花苜蓿叶片的光破坏防御作用[J]. 草地学报，2024，32（5）：1429-1439
[4] LIU H，SONG S B，ZHANG H，et al. Signaling transduction of ABA， ROS， and Ca²⁺ in plant stomatal closure in response to drought[J]. International Journal of Molecular Sciences，2022，23（23）：14824
[5] JIA W S，ZHANG J H. Stomatal movements and long-distance signaling in plants[J]. Plant Signaling & Behavior，2008，3（10）：772-777
[6] LV Y P，GU L H，MAN R Z，et al. Response of stomatal conductance， transpiration， and photosynthesis to light and CO₂ for rice leaves with different appearance days[J]. Frontiers in Plant Science，2024，15：1397948
[7] LIU Y M，CHEN S H，WEI P P，et al. A briefly overview of the research progress for the abscisic acid analogues[J]. Frontiers in Chemistry，2022，10：967404
[8] 徐剑，杨帆，孙正海. 不同预处理对喀西茄种子萌发及S-诱抗素对幼苗生长的影响[J]. 种子，2023，42（9）：93-97，104
[9] 姚晨涛，张风文，李刚，等. S-诱抗素拌种对干旱胁迫下玉米生长调控作用[J]. 玉米科学，2020，28（4）：67-73
[10] YANG T，LV R，LI J H，et al. Phytochrome A and B negatively regulate salt stress tolerance of Nicotiana tobacum via ABA-jasmonic acid synergistic cross-talk[J]. Plant & Cell Physiology，2018，59（11）：2381-2393
[11] 罗鑫，闫利军，李达旭，等. 野生垂穗披碱草种质资源抗旱性筛选与评价[J]. 草地学报，2024，32（7）：2158-2168
[12] 王传旗，武俊喜，余成群，等. 环境因子对西藏巴青县野生垂穗披碱草种子萌发的影响[J]. 草地学报，2017，25（5）：1103-1107
[13] 李合生. 植物生理生化实验原理和技术[M]. 北京：高等教育出版社，2000：134-261
[14] 黄安琪，马国辉，赵黎明，等. S-诱抗素浸种对盐胁迫下水稻幼苗生长及生理特性的影响[J]. 杂交水稻，2023，38（2）：124-134
[15] 武建宽，秦焕荣，杨萍. S-诱抗素对小麦生长调节作用及增产效果试验研究[J]. 陕西农业科学，2014，60（2）：6-7
[16] ROWE J H，TOPPING J F，LIU J L，et al. Abscisic acid regulates root growth under osmotic stress conditions via an interacting hormonal network with cytokinin， ethylene and auxin[J]. New Phytologist，2016，211（1）：225-239
[17] 吴琼，丁凯鑫，余明龙，等. 新型植物生长调节剂B2对玉米光合荧光特性及产量的影响[J]. 作物杂志，2020（5）：174-181
[18] 杨朝伟，安明珠，任伟，等. 两个黑麦品种种子萌发期和幼苗期耐寒性差异及其生理机制[J]. 中国草地学报，2024，46（6）：1-9
[19] GAO S H，WANG Y F，ZENG Z，et al. Integrated bioinformatic and physiological analyses reveal the pivotal role of hydrogen sulfide in enhancing low-temperature tolerance in alfalfa[J]. Physiologia Plantarum，2023，175（2）：e13885
[20] 关思慧，柴亚倩，杨元玲，等.低温胁迫对石榴光合特性和抗氧化能力的影响[J]. 中国农学通报，2024，40（3）：66-75
[21] 蒋文鑫，马国辉，赵黎明，等.S-诱抗素对不同盐胁迫下水稻生理特性及产量的影响[J]. 核农学报，2023，37（12）：2485-2495
[22] OSMOND B，BADGER M，MAXWELL K，et al. Too many photons： photorespiration， photoinhibition and photooxidation[J]. Trends in Plant Science，1997，2（4）：119-121
[23] MARRI L，SPARLA F，PUPILLO P，et al. Co-ordinated gene expression of photosynthetic glyceraldehyde-3-phosphate dehydrogenase， phosphoribulokinase， and CP12 in Arabidopsis thaliana[J]. Journal of Experimental Botany，2005，56（409）：73-80
[24] 刘博文. 多效唑对高羊茅耐荫性的调控作用[D]. 杨凌：西北农林科技大学，2023：4-71
[25] 何昕孺，肖庆红，秦垦，等. S-诱抗素对枸杞叶片生长和光合生理参数的影响[J]. 宁夏农林科技，2016，57（3）：1-3
[26] 吴焜玥，张燕，蒙静，等. S-诱抗素和新奥霉素在日光温室黄瓜栽培中的应用研究[J]. 农业科学研究，2013，34（4）：39-43
[27] TAN Y Q， YANG Y， SHEN X， et al. Multiple cyclic nucleotide-gated channels function as ABA-activated Ca²⁺ channels required for ABA-induced stomatal closure in Arabidopsis[J]. The Plant Cell，2023，35（1）：239-259

Effects of S-ABA on Photosynthesis Characteristic of Elymus nutans Under Combined Low Temperature and Drought Stresses

S-诱抗素对低温干旱复合胁迫下垂穗披碱草光合特征的影响

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