Effects of Different Grazing Intensity on Soil Bacterial Community in Inner Mongolian Desert Grasslands

doi:10.11733/j.issn.1007-0435.2025.06.006

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (6): 1764-1770.DOI: 10.11733/j.issn.1007-0435.2025.06.006

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Effects of Different Grazing Intensity on Soil Bacterial Community in Inner Mongolian Desert Grasslands

ZHOU Hui-min¹, GAO Zhi-wei¹, ZHANG Xiao-jia¹, WU Jia-xin¹, WANG Wei-feng², HAN Guo-dong¹

1. College of Grassland Science, Inner Mongolia Agricultural University, Key Laboratory of Grassland Resources, Ministry of Education of China, Hohhot, Inner Mongolia 010011, China;
2. Inner Mongolia Autonomous Region Forestry Scientific Research Institute, Hohhot, Inner Mongolia 010010, China

Received:2024-08-19 Revised:2024-10-08 Online:2025-06-15 Published:2025-06-13

不同放牧强度对内蒙古荒漠草原土壤细菌群落的影响

周慧敏¹, 高志伟¹, 张晓嘉¹, 吴佳芯¹, 王伟峰², 韩国栋¹

1. 内蒙古农业大学草业学院/内蒙古农业大学草地资源教育部重点实验室, 内蒙古呼和浩特 010011;
2. 内蒙古自治区林业科学研究院, 内蒙古呼和浩特 010010

通讯作者: 韩国栋,E-mail:hanguodong@imau.edu.cn
作者简介:周慧敏（1997-），女，汉族，内蒙古呼和浩特人，硕士研究生，主要从事土壤微生物研究，E-mail：15661119961@163.com
基金资助:
内蒙古自治区林草局揭榜挂帅项目“草原保护与可持续管理碳汇方法学研发与应用”（2024LKY-TH02）；以铸牢中华民族共同体意识为工作主线推进内蒙古生态文明建设研究（ZLJD24034）资助

Abstract

Abstract: To study the response of soil bacterial community to different grazing intensities in Inner Mongolia desert grassland， and to provide scientific， theoretical and technical support for the ecological protection and restoration of desert grassland. Taking the Stipa breviflora desert grassland in Inner Mongolia as the research object， the effects of different grazing intensities （no grazing， light grazing， medium grazing and heavy grazing） on soil physicochemical properties as well as the composition and diversity of soil bacterial communities were studied， and the interactions between soil physicochemical properties and soil bacterial communities were analyzed. The results showed that heavy grazing reduced total nitrogen content， nitrate nitrogen content， ammonium nitrogen content， available phosphorus content and soil moisture of Inner Mongolian desert grassland soils compared with no grazing. Light grazing reduced the abundance of Gemmatomonadota. There was no change in the dominant phylum of soil bacteria and no change in α-diversity with increasing grazing intensity. Light grazing changed the bacterial β-diversity. The results of the study can help to understand the ecological sensitivity of soil microorganisms and provide scientific guidance for achieving sustainable management of desert grassland ecosystems.

Key words: Desert grassland, Grazing intensity, Bacterial community, Soil physicochemical properties

摘要： 研究内蒙古荒漠草原土壤细菌群落对不同放牧强度的响应，为荒漠草原的生态保护和恢复提供科学、理论和技术支持。以内蒙古短花针茅（Stipa breviflora）荒漠草原为研究对象，研究了不同放牧强度（不放牧、轻度放牧、中度放牧、重度放牧）对土壤理化性质以及土壤细菌群落组成和多样性的影响，分析了土壤理化性质与土壤细菌群落的相互作用。结果表明：与不放牧相比，重度放牧降低了内蒙古荒漠草原土壤的全氮、硝态氮、铵态氮、速效磷含量及土壤湿度。轻度放牧降低了芽单胞菌门的丰度。随放牧强度的增加，土壤细菌的优势门无变化，α-多样性无变化。轻度放牧改变了细菌β-多样性。研究结果有助于理解土壤微生物的生态敏感性，为实现荒漠草原生态系统的可持续管理提供科学指导。

关键词: 荒漠草原, 放牧强度, 细菌群落, 土壤理化性质

CLC Number:

S812.2

ZHOU Hui-min, GAO Zhi-wei, ZHANG Xiao-jia, WU Jia-xin, WANG Wei-feng, HAN Guo-dong. Effects of Different Grazing Intensity on Soil Bacterial Community in Inner Mongolian Desert Grasslands[J]. Acta Agrestia Sinica, 2025, 33(6): 1764-1770.

周慧敏, 高志伟, 张晓嘉, 吴佳芯, 王伟峰, 韩国栋. 不同放牧强度对内蒙古荒漠草原土壤细菌群落的影响[J]. 草地学报, 2025, 33(6): 1764-1770.

References

[1] GIBSON D J. Grasses and grassland ecology[M]. Oxford ：Oxford University Press，2009：27
[2] DENG Y H，LI X L，DING M K，et al. Characteristics of water retention nutrient storage and biomass production across alpine grassland soils in the Qilian Mountains[J]. Geoderma Regional，2023（35）：e00721
[3] LIU K S，SHAO X Q. Grassland ecological management and utilization for sustainability[J]. Agronomy，2024，14（1）：149
[4] 国家统计局. 第三次全国国土调查主要数据公报[N]. 人民日报，2021-8-27（17）
[5] 内蒙古自治区人民政府. 7.3亿亩内蒙古最新划定基本草原面积[N]. 内蒙古日报，2024-01-17（01）
[6] 卞莹莹，张志敏，付镇，等.荒漠草原区不同植被恢复模式土壤微生物菌落分布特征及其与土壤理化性质的相关性[J].草地学报，2021，29（4）：655-663
[7] GEA A B，HAUTIER Y，GEISEN S. Interactive effects of global change drivers as determinants of the link between soil biodiversity and ecosystem functioning[J]. Global Change Biology，2023，29（2）：296-307
[8] MORRISSEY E M，KANE J，TRIPATHI B M，et al. Carbon acquisition ecological strategies to connect soil microbial biodiversity and carbon cycling[J]. Soil Biology and Biochemistry，2023（177）：108893
[9] XUE P，MINASNY B，MCBRATNEY A，et al. Distinctive role of soil type and land use in driving bacterial communities and carbon cycling functions down soil profiles[J]. Catena，2023（223）：106903
[10] LUO Z，LIU J，JIA T，et al. Soil bacterial community response and nitrogen cycling variations associated with subalpine meadow degradation on the Loess Plateau，China[J]. Applied and Environmental Microbiology，2020，86（9）：1128
[11] HERMANS S M，BUCKLEY H L，CASE B S，et al. Using soil bacterial communities to predict physico-chemical variables and soil quality[J]. Microbiome，2020，8（1）：79
[12] 付莉娇，李雪琴，范继辉，等.藏北高寒草原典型植物根际土壤细菌群落结构多样性及根系特征分析[J].草地学报，2022，30（5）：1131-1140
[13] L J，CHEN H S，CHEN D M，et al. Soil biota diversity and plant diversity both contributed to ecosystem stability in grasslands[J]. Ecology Letters，2023，26（6）：858-868
[14] WANG Y，GUO M，LI Y，et al. Responses of soil bacterial communities and chemical properties to grazing regulation in desert steppe[J]. Agronomy，2023，13（11）：2817
[15] LIU N，ZHANG Y，CHANG S，et al. Impact of grazing on soil carbon and microbial biomass in typical steppe and desert steppe of Inner Mongolia[J]. Plos One，2012，7（5）：36434
[16] MATINIZADEH M，NOURI E，BAYRANVAND M，et al. Arbuscular mycorrhiza and rhizosphere soil enzymatic activities as modulated by grazing intensity and plant species identity in a semi-arid grassland[J]. Rhizosphere，2024（30）：100893
[17] GUO Y，LIAO H L，BOUGHTON E H，et al. Effects of land-use intensity，grazing and fire disturbances on soil bacterial and fungal communities in subtropical wetlands[J]. Agriculture，Ecosystems & Environment，2023（345）：108314
[18] INSAM H，RANGGER A，HENRICH M，et al. The effect of grazing on soil microbial biomass and community on Alpine pastures[J]. Annales Rei Botanice，1996（36）：205-216
[19] DING L，TIAN L L，LI J Y，et al. Grazing lowers soil multifunctionality but boosts soil microbial network complexity and stability in a subtropical grassland of China[J]. Frontiers in Microbiology，2023（13）：1027097
[20] ZHAO F，REN C，SHELTON S，et al. Grazing intensity influence soil microbial communities and their implications for soil respiration[J]. Agriculture，Ecosystems & Environment，2017（249）：50-56
[21] GONG X，WANG Y，ZHAN T，et al. Advances in meta-analysis of the effects of grazing on grassland ecosystems in China[J]. Agriculture，2023，13（5）；1084
[22] YANG Y，ZHANG H，LIU W，et al. Effects of grazing intensity on diversity and composition of rhizosphere and non-rhizosphere microbial communities in a desert grassland[J]. Ecology and Evolution，2023，13（7）：e10300
[23] ZHANG H，FU G. Responses of plant，soil bacterial and fungal communities to grazing vary with pasture seasons and grassland types northern Tibet[J]. Land Degradation & Development，2021，32（4）：1821-1832
[24] ZHOU X Q，WANG J Z，HAO Y B，et al. Intermediate grazing intensities by sheep increase soil bacterial diversities in an Inner Mongolian steppe[J]. Biology and Fertility of Soils，2010，46（8）：817-824
[25] RONG Y P，MONACO T A，LIU Z K，et al. Soil microbial community structure is unaltered by grazing intensity and plant species richness in a temperate grassland steppe in northern China[J]. European Journal of Soil Biology，2022（110）：103404
[26] YANG Z C，NOLTE S，WU J H. Tidal flooding diminishes the effects of livestock grazing on soil micro-food webs in a coastal saltmarsh[J]. Agriculture，Ecosystems & Environment，2017（236）：177-186
[27] NORTHUP B K，BROWN J R，HOLT J A. Grazing impacts on the spatial distribution of soil microbial biomass around tussock grasses in a tropical grassland[J]. Applied Soil Ecology，1999，13（3）：259-270
[28] LAMB E G，KENNEDY N，SICILIANO S D. Effects of plant species richness and evenness on soil microbial community diversity and function[J]. Plant and Soil，2011，338（1）：483-495
[29] ZHENG Q，HU Y T，ZHANG S S，et al. Soil multifunctionality is affected by the soil environment and by microbial community composition and diversity[J]. Soil Biology and Biochemistry，2019（136）：107521
[30] TEAGUE R，DOWHOWER S. Links of microbial and vegetation communities with soil physical and chemical factors for a broad range of management of tallgrass prairie[J]. Ecological Indicators，2022（142）：109280
[31] CHENG H Y，WU B D，WEI M，et al. Changes in community structure and metabolic function of soil bacteria depending on the type restoration processing in the degraded alpine grassland ecosystems in Northern Tibet[J]. Science of the Total Environment，2021（755）：142619
[32] WANG G，ZHANG L H，GUO Z H，et al. Benefits of biological nitrification inhibition of Leymus chinensis under alkaline stress：the regulatory function of ammonium-N exceeds its nutritional function[J]. Frontiers in Plant Science，2023（14）：1145830
[33] BLUME H P，BRÜMMER G W，FLEIGE H，et al. Soil organisms and their habitat[M]//Scheffer/SchachtschabelSoil science. Berlin，Heidelberg：Springer Berlin Heidelberg，2015：87-122
[34] XU H W，YOU C M，TAN B，et al. Effects of livestock grazing on the relationships between soil microbial community and soil carbon in grassland ecosystems[J]. Science of the Total Environment，2023（881）：163416
[35] WANG Z N，YUAN X，WANG D L，et al. Large herbivores influence plant litter decomposition by altering soil properties and plant quality in a meadow steppe[J]. Scientific Reports，2018，8（1）：9089
[36] QIN Y Y，ZHANG X F，ADAMOWSKI J F，et al. Grassland grazing management altered soil properties and microbial β-diversity but not α-diversity on the Qinghai-Tibetan Plateau[J]. Applied Soil Ecology，2021（167）：104032
[37] JACOBY R，PEUKERT M，SUCCURRO A，et al. The role of soil microorganisms in plant mineral nutrition-current knowledge and future directions[J]. Frontiers in Plant Science，2017（8）：1617
[38] POOS M S，WALKER S C，JACKSON D A. Functional-diversity indices can be driven by methodological choices and species richness[J]. Ecology，2009，90（2）：341-347
[39] DYKHUIZEN D. Experimental studies of natural selection in bacteria[J]. Annual Review of Ecology and Systematics，1990（21）：373-398
[40] GAO Z W，HAN C W，HUANG J，et al. Exploring the optimal grazing intensity in desert steppe based on soil nematode community and function[J]. Land Degradation & Development，2022，33（14）：2512-2527
[41] JIANG H M，CHEN Y C，HU Y，et al. Soil bacterial communities and diversity in alpine grasslands on the Tibetan Plateau based on 16S rRNA gene sequencing[J]. Frontiers in Ecology and Evolution，2021（9）：630722
[42] WILLIAMS S T，CROSS T. Chapter XI actinomycetes[M]//Amsterdam：Elsevier，1971：295-334
[43] GITARI J M，MURAYA M M，ONYANGO B O，et al. The influence of soil’s physicochemical properties and land use systems on the abundance of actinomycetes populations[J]. South Asian Journal of Research in Microbiology，2023，16（2）：20-37
[44] FAWAZ M N. Revealing the ecological role of gemmatimonadetes through cultivation and molecular analysis of agricultural soils[D].Tennessee： University of Tennessee，2013：6-14

Effects of Different Grazing Intensity on Soil Bacterial Community in Inner Mongolian Desert Grasslands

不同放牧强度对内蒙古荒漠草原土壤细菌群落的影响

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