Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum

doi:10.11733/j.issn.1007-0435.2025.07.006

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (7): 2099-2113.DOI: 10.11733/j.issn.1007-0435.2025.07.006

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Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum

FANG Ting¹, LUO Jia-jia^1,2, ZHANG Yu-ge¹, MAO Yu-ye¹, GUO Yu-tong¹, DONG Rong-shu², CAI Ze-ping¹

1. School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan Province 570228, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China

Received:2024-09-10 Revised:2024-10-17 Online:2025-07-15 Published:2025-07-18

象草PHR家族基因的鉴定及表达分析

方婷¹, 罗佳佳^1,2, 张语歌¹, 毛昱烨¹, 郭雨彤¹, 董荣书², 蔡泽坪¹

1. 海南大学热带农林学院, 海南海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室, 海南海口 571101

通讯作者: 蔡泽坪,E-mail:992995@hainanu.edu.cn
作者简介:方婷（1997-），女，汉族，江西赣州人，硕士研究生，主要从事草业科学方面研究，E-mail：ftft014845@163.com；罗佳佳（1988-），女，汉族，四川遂宁人，副研究员，主要从事草业科学方面研究，E-mail：luojiajia09skj@163.com
基金资助:
海南省自然科学基金高层次人才项目（322RC772）；海南省科协青年科技英才创新计划项目（QCQTXM202201）；国家自然科学基金地区科学基金项目（32460348）；国家自然科学基金青年科学基金项目（32401498）；海南大学国家级大学生创新创业训练计划项目（202410589092）；财政部和农业农村部：国家现代农业产业技术体系（CARS-34）资助

Abstract

Abstract: The phosphate starvation response （PHR） factor plays a pivotal role in regulating phosphorus homeostasis in plants， yet the PHRs in Pennisetum purpureum （elephant grass） remain unreported. This study analyzed the effects of low phosphorus （P） stress on the growth of elephant grass， identified the members of PHR family in elephant grass， and examined their transcription expression levels. Furthermore， the two key genes PpPHR8/13 were cloned， and their expression patterns and subcellular localization were analyzed. The results demonstrated that low P stress significantly inhibited the growth of elephant grass. A total of 42 PpPHRs were identified from the elephant grass genome， with collinear gene pairs originating from whole-genome duplication or segmental duplication events. Transcriptome showed that 15 PpPHRs were significantly upregulated by low P stress in elephant grass leaves （and/or roots）， with PpPHR8/13 exhibiting relatively high expression levels. Additionally， tissue-specific expression analysis revealed that PpPHR8/13 had the highest expression levels in roots and stems， respectively. Quantitative PCR confirmed that low P treatment enhanced the expression of PpPHR8/13 in both roots and leaves. Subcellular localization indicated that both PpPHR8 and PpPHR13 proteins were localized in the nucleus. This study provides candidate gene resources for elucidating the mechanisms underlying elephant grass's response to phosphorus deficiency.

Key words: Pennisetum purpureum, Low phosphorus stress, Phosphate starvation response factor, Gene expression, Subcellular localization

摘要： 磷饥饿响应因子PHR（Phosphate starvation response，PHR）在调节植物磷稳态中发挥重要作用，但象草（Pennisetum purpureum）PHR仍未被报道。本研究分析了低磷胁迫对象草生长的影响，鉴定了象草PHR家族成员，检测了转录表达水平。同时，克隆了两个关键基因PpPHR8/13，分析了表达模式和亚细胞定位。结果表明：低磷胁迫显著抑制象草生长；象草全基因组鉴定到42个PpPHRs，而共线性基因对来源于全基因组复制或片段复制事件；转录组中，15个PpPHRs在象草叶片（或/和根系）中受低磷诱导显著上调表达，且PpPHR8/13相对表达量较高。此外，组织表达显示PpPHR8/13分别在根系和茎中的表达量最高；定量PCR显示低磷处理增强了PpPHR8/13在根系和叶片中的表达；亚细胞定位显示PpPHR8/13蛋白均定位于细胞核。本研究结果为象草响应磷养分缺乏的机理提供了候选基因资源。

关键词: 象草, 低磷胁迫, 磷饥饿响应因子, 基因表达, 亚细胞定位

CLC Number:

FANG Ting, LUO Jia-jia, ZHANG Yu-ge, MAO Yu-ye, GUO Yu-tong, DONG Rong-shu, CAI Ze-ping. Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum[J]. Acta Agrestia Sinica, 2025, 33(7): 2099-2113.

方婷, 罗佳佳, 张语歌, 毛昱烨, 郭雨彤, 董荣书, 蔡泽坪. 象草PHR家族基因的鉴定及表达分析[J]. 草地学报, 2025, 33(7): 2099-2113.

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Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum

象草PHR家族基因的鉴定及表达分析

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